(Circular) (Six-base) MAPSORT of: pFN21AE5450.seq Check: 1764 from: 1 to: 5716 pFN21AE5450 5716 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 2529 2639 3779 5163 5431 1632 Size: 471 426 110 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 471 426 268 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2252 1184 Size: 1068 4648 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2157 3025 3251 3403 5512 1493 Size: 664 868 226 152 2109 1697 Fragments arranged by size: 2109 1697 868 664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3037 4492 618 Size: 325 217 627 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 627 325 217 BanII G_rGCy'C Cuts at: 1391 3035 1391 Size: 1644 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2329 2643 928 Size: 971 430 314 4001 Fragments arranged by size: 4001 971 430 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4327 136 Size: 107 122 71 631 605 2655 1525 Fragments arranged by size: 2655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 2205 3133 2205 Size: 928 4788 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 4405 442 Size: 416 53 83 399 24 2030 958 1753 Fragments arranged by size: 2030 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2213 1056 Size: 1157 4559 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2338 3841 4706 5004 2338 Size: 1503 865 298 3050 Fragments arranged by size: 3050 1503 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2518 3507 493 Size: 711 1314 989 2702 Fragments arranged by size: 2702 1314 989 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3523 3905 4540 275 Size: 53 83 186 936 1990 382 635 1451 Fragments arranged by size: 1990 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 1178 Size: 30 165 30 1467 30 3994 Fragments arranged by size: 3994 1467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 1186 Size: 1195 4521 BseYI C'CCAG_C Cuts at: 4919 5415 4919 Size: 496 5220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2368 3777 3862 4933 2368 Size: 1409 85 1071 3151 Fragments arranged by size: 3151 1409 1071 85 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 3035 3777 3862 4933 1391 Size: 399 578 667 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 667 578 399 85 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 2736 2775 3605 5335 2736 Size: 39 830 1730 3117 Fragments arranged by size: 3117 1730 830 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 3071 844 Size: 1424 803 3489 Fragments arranged by size: 3489 1424 803 BsrBI CCG'CTC Cuts at: 1394 1823 3603 1394 Size: 429 1780 3507 Fragments arranged by size: 3507 1780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 4211 4385 65 Size: 1183 906 2057 174 1396 Fragments arranged by size: 2057 1396 1183 906 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstYI r'GATC_y Cuts at: 1972 2880 2988 3046 3413 3798 3815 5267 5353 Size: 908 108 58 367 385 17 1452 86 Cuts at: 5353 5365 5711 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 908 385 367 346 108 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2138 2205 2236 3232 4026 4046 2138 Size: 67 31 996 794 20 3808 Fragments arranged by size: 3808 996 794 67 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 2691 3020 2691 Size: 329 5387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2847 3163 Size: 54 1425 50 275 18 249 768 316 Cuts at: 3163 4052 5681 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 768 316 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3646 1353 Size: 2293 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4303 4677 4823 387 Size: 1064 138 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2103 2529 2639 3779 4376 5163 5431 Size: 393 471 426 110 1140 597 787 268 Cuts at: 5431 1239 Size: 1524 Fragments arranged by size: 1524 1140 787 597 471 426 393 268 110 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2992 2992 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2320 2352 4357 4389 4431 Size: 32 135 32 748 32 2005 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 2005 748 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2529 3060 3306 669 Size: 988 281 591 531 246 3079 Fragments arranged by size: 3079 988 591 531 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2849 10 Size: 54 1425 343 249 768 2877 Fragments arranged by size: 2877 1425 768 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 3674 4033 4192 518 Size: 648 2508 359 159 2042 Fragments arranged by size: 2508 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 3132 3809 4957 5202 1606 Size: 660 866 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 677 660 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 2209 1052 Size: 1157 4559 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5716 PfoI T'CCnGG_A Cuts at: 2712 2712 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 2265 3068 830 Size: 1435 803 3478 Fragments arranged by size: 3478 1435 803 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2266 2266 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 2224 2261 3064 4199 4880 5071 826 Size: 219 1179 37 803 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 803 681 219 191 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2317 3820 4721 4983 820 Size: 197 926 374 1503 901 262 1553 Fragments arranged by size: 1553 1503 926 901 374 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 3155 513 Size: 552 2090 3074 Fragments arranged by size: 3074 2090 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3962 5674 96 Size: 274 80 33 51 1443 1985 1712 138 Fragments arranged by size: 1985 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 5676 5708 2326 Size: 32 3318 32 2334 Fragments arranged by size: 3318 2334 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 2451 2823 3845 2451 Size: 372 1022 4322 Fragments arranged by size: 4322 1022 372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI