################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I FauI FseI FspI HaeII HgaI Hin1I KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 413 GGTACC G^GTACC AcsI 1290 GAATTC [GA]^AATT[CT] AgeI 416 ACCGGT A^CCGGT ApaI 1076 GGGCCC GGGCC^C ApoI 1290 GAATTC [GA]^AATT[CT] Asp718I 413 GGTACC G^GTACC BclI 752 TGATCA T^GATCA BsaWI 416 ACCGGT [AT]^CCGG[AT] BsgI 443 GTGCAG GTGCAG(16/14) BsiEI 889 CGGTCG CG[GA][CT]^CG BsmAI 194 GTCTC GTCTC(1/5) BstBI 654 TTCGAA TT^CGAA BstXI 503 CCATCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 500 GCGC GCG^C DsaI 476 CCACGG C^C[GA][CT]GG EaeI 1034 TGGCCG [CT]^GGCC[GA] EcoRI 1290 GAATTC G^AATTC EcoT22I 122 ATGCAT ATGCA^T FbaI 752 TGATCA T^GATCA HhaI 500 GCGC GCG^C HinP1I 500 GCGC G^CGC HindIII 436 AAGCTT A^AGCTT HpaI 396 GTTAAC GTT^AAC KpnI 413 GGTACC GGTAC^C MspA1I 886 CAGCGG C[AC]G^C[GT]G NsiI 122 ATGCAT ATGCA^T NspI 961 GCATGC [GA]CATG^[CT] NspV 654 TTCGAA TT^CGAA PflMI 631 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 416 ACCGGT A^CCGGT PpuMI 910 GGGTCCT [GA]G^G[AT]CC[CT] PstI 313 CTGCAG CTGCA^G SacI 33 GAGCTC GAGCT^C SalI 139 GTCGAC G^TCGAC SfuI 654 TTCGAA TT^CGAA SpeI 1131 ACTAGT A^CTAGT SphI 961 GCATGC GCATG^C Van91I 631 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 139 GTCGAC GT^[AC][GT]AC 1135 GTCTAC AfaI 414 GTAC GT^AC 1194 GTAC AlwI 894 GATCC GGATC(4/5) 1100 GGATC AspHI 33 GAGCTC G[AT]GC[AT]^C 466 GTGCTC BanII 33 GAGCTC G[GA]GC[CT]^C 1076 GGGCCC BbsI 867 GTCTTC GAAGAC(2/6) 982 GTCTTC BpuAI 867 GTCTTC GAAGAC(2/6) 982 GTCTTC BsaJI 476 CCACGG C^C[ACGT][ACGT]GG 907 CCAGGG BsiHKAI 33 GAGCTC G[AT]GC[AT]^C 466 GTGCTC BsmFI 1278 GGGAC GGGAC(10/14) 297 GTCCC BsmI 318 GAATGC GAATGC(1/-1) 1353 GCATTC BsrFI 416 ACCGGT [GA]^CCGG[CT] 1242 ACCGGC Cac8I 434 GCAAGC GC[ACGT]^[ACGT]GC 961 GCATGC Cfr10I 416 ACCGGT [GA]^CCGG[CT] 1242 ACCGGC DdeI 569 CTAAG C^T[ACGT]AG 854 CTCAG HapII 417 CCGG C^CGG 1243 CCGG HpaII 417 CCGG C^CGG 1243 CCGG MaeII 464 ACGT A^CGT 521 ACGT MaeIII 342 GTCAC ^GT[ACGT]AC 777 GTCAC MspI 417 CCGG C^CGG 1243 CCGG PleI 142 GACTC GAGTC(4/5) 1051 GAGTC RsaI 414 GTAC GT^AC 1194 GTAC SapI 468 GCTCTTC GCTCTTC(1/4) 879 GCTCTTC TfiI 534 GAATC G^A[AT]TC 652 GATTC Tsp45I 342 GTCAC ^GT[GC]AC 777 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BfaI BmyI BpmI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cfr13I DpnII DraII DrdI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp509I TspRI TthHB8I