(Circular) (Six-base) MAPSORT of: pFN21AB5802.seq Check: 2102 from: 1 to: 6118 pFN21AB5802 6118 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 3141 3461 1937 Size: 208 996 320 4594 Fragments arranged by size: 4594 996 320 208 Acc65I G'GTAC_C Cuts at: 2418 3439 2418 Size: 1021 5097 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2859 3105 3384 4181 5565 5833 1632 Size: 1227 246 279 797 1384 268 1917 Fragments arranged by size: 1917 1384 1227 797 279 268 246 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 2421 4949 2421 Size: 2528 3590 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 2194 1166 Size: 1028 5090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 3861 5433 3861 Size: 1572 4546 ApaI G_GGCC'C Cuts at: 3085 3085 Size: 6118 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 3295 3427 3653 3805 5914 1493 Size: 1802 132 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 132 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 2418 3012 3439 4894 Size: 325 217 627 394 237 594 427 1455 Cuts at: 4894 618 Size: 1842 Fragments arranged by size: 1842 1455 627 594 427 394 325 237 217 BanII G_rGCy'C Cuts at: 1391 2042 3085 3437 1391 Size: 651 1043 352 4072 Fragments arranged by size: 4072 1043 651 352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2865 2980 928 Size: 971 966 115 4066 Fragments arranged by size: 4066 971 966 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 2757 1548 Size: 1209 4909 BfrBI ATG'CAT Cuts at: 2129 2129 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 3017 3085 4179 5335 1790 Size: 1227 68 1094 1156 2573 Fragments arranged by size: 2573 1227 1156 1094 68 BmgBI CAC'GTC Cuts at: 1318 2470 1318 Size: 1152 4966 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2201 2551 2771 4778 1239 Size: 888 74 350 220 2007 2579 Fragments arranged by size: 2579 2007 888 350 220 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4243 5108 5406 4243 Size: 865 298 4955 Fragments arranged by size: 4955 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 4551 4949 5223 5370 5781 2421 Size: 2130 398 274 147 411 2758 Fragments arranged by size: 2758 2130 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2199 2229 2900 2930 1178 Size: 30 165 30 796 30 671 30 4366 Fragments arranged by size: 4366 796 671 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6118 BseYI C'CCAG_C Cuts at: 2371 2977 5321 5817 2371 Size: 606 2344 496 2672 Fragments arranged by size: 2672 2344 606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2469 1163 Size: 573 733 4812 Fragments arranged by size: 4812 733 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2897 3568 4329 5357 1540 Size: 275 186 896 671 761 1028 2301 Fragments arranged by size: 2301 1028 896 761 671 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2475 4179 4264 5335 2042 Size: 433 1704 85 1071 2825 Fragments arranged by size: 2825 1704 1071 433 85 BsmI GAATG_Cn' Cuts at: 2329 3358 3629 3722 2329 Size: 1029 271 93 4725 Fragments arranged by size: 4725 1029 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2475 3017 3085 3437 4179 4264 Size: 399 252 433 542 68 352 742 85 Cuts at: 4264 5335 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 542 433 399 352 252 85 68 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2592 2959 3070 3218 4613 4787 65 Size: 1183 1344 367 111 148 1395 174 1396 Fragments arranged by size: 1396 1395 1344 1183 367 174 148 111 BsrFI r'CCGG_y Cuts at: 1700 1810 2421 3247 4762 4949 1700 Size: 110 611 826 1515 187 2869 Fragments arranged by size: 2869 1515 826 611 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 2660 4922 2660 Size: 2262 3856 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113 Size: 1476 367 385 17 1452 86 12 346 Cuts at: 6113 1972 Size: 1977 Fragments arranged by size: 1977 1476 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 513 Size: 552 939 477 4150 Fragments arranged by size: 4150 939 552 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2304 3634 4428 4448 2304 Size: 1330 794 20 3974 Fragments arranged by size: 3974 1330 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 2941 3166 5125 809 Size: 507 1209 416 225 1959 1802 Fragments arranged by size: 1959 1802 1209 507 416 225 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3039 3565 4454 Size: 54 1425 50 275 18 1209 526 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1209 889 526 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 2891 3124 3360 4048 1353 Size: 1127 411 233 236 688 3423 Fragments arranged by size: 3423 1127 688 411 236 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2040 2040 Size: 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2201 2551 2771 2859 3105 3384 Size: 393 495 74 350 220 88 246 279 Cuts at: 3384 4181 4778 5565 5833 1239 Size: 797 597 787 268 1524 Fragments arranged by size: 1524 797 787 597 495 393 350 279 268 246 220 88 74 EcoO109I rG'GnC_Cy Cuts at: 2916 3081 3082 2916 Size: 165 1 5952 Fragments arranged by size: 5952 165 1 EcoRI G'AATT_C Cuts at: 3295 3427 3805 3295 Size: 132 378 5608 Fragments arranged by size: 5608 378 132 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2900 2932 3097 Size: 32 135 32 515 32 781 32 165 Cuts at: 3097 3129 4759 4791 4833 4865 1373 Size: 32 1630 32 42 32 2626 Fragments arranged by size: 2626 1630 781 515 165 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2403 3130 3462 3708 669 Size: 988 281 208 257 727 332 246 3079 Fragments arranged by size: 3079 988 727 332 281 257 246 208 HindIII A'AGCT_T Cuts at: 748 2441 748 Size: 1693 4425 HpaI GTT'AAC Cuts at: 2403 3708 2403 Size: 1305 4813 KpnI G_GTAC'C Cuts at: 2422 3443 2422 Size: 1021 5097 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2194 2297 2390 2753 4076 4435 4594 Size: 648 1028 103 93 363 1323 359 159 Cuts at: 4594 518 Size: 2042 Fragments arranged by size: 2042 1323 1028 648 363 359 159 103 93 MspA1I CmG'CkG Cuts at: 1606 2893 3534 4211 5359 5604 1606 Size: 1287 641 677 1148 245 2120 Fragments arranged by size: 2120 1287 1148 677 641 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NsiI A_TGCA'T Cuts at: 2131 2131 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2970 3476 5021 1132 Size: 97 1741 506 1545 2229 Fragments arranged by size: 2229 1741 1545 506 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2642 1108 Size: 1534 4584 PfoI T'CCnGG_A Cuts at: 2217 2217 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2916 2916 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 3081 3081 Size: 6118 PstI C_TGCA'G Cuts at: 830 2322 3470 830 Size: 1492 1148 3478 Fragments arranged by size: 3478 1492 1148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 SacI G_AGCT'C Cuts at: 2042 2042 Size: 6118 SalI G'TCGA_C Cuts at: 1936 2144 3460 1936 Size: 208 1316 4594 Fragments arranged by size: 4594 1316 208 SapI GCTCTTCn'nnn_ Cuts at: 2480 2891 2480 Size: 411 5707 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2086 2318 2648 3163 3466 4601 5282 Size: 219 1041 232 330 515 303 1135 681 Cuts at: 5282 5473 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1041 681 515 330 303 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4222 5123 5385 820 Size: 197 926 2279 901 262 1553 Fragments arranged by size: 2279 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 3136 152 Size: 2984 3134 SphI G_CATG'C Cuts at: 1132 2970 3476 1132 Size: 1838 506 3774 Fragments arranged by size: 3774 1838 506 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2004 3557 513 Size: 552 939 1553 3074 Fragments arranged by size: 3074 1553 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 4162 4315 4500 4945 325 Size: 1255 2565 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3198 4364 6076 Size: 274 80 33 51 1443 1221 1166 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 1712 1443 1221 1166 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI