################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 5 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 339 ATTAAT AT^TAAT AsnI 339 ATTAAT AT^TAAT BclI 357 TGATCA T^GATCA BfaI 1141 CTAG C^TAG BglII 1188 AGATCT A^GATCT BseRI 818 GAGGAG GAGGAG(10/8) BsgI 842 GTGCAG GTGCAG(16/14) BsmFI 242 GTCCC GGGAC(10/14) BsrDI 197 GCAATG GCAATG(2/0) BsrI 874 CCAGT ACTGG(1/-1) BssKI 323 CCAGG ^CC[ACGT]GG BstNI 323 CCAGG CC^[AT]GG BstXI 662 CCAAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 513 GCCAGC GC[ACGT]^[ACGT]GC DraI 427 TTTAAA TTT^AAA DraII 1014 AGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 1006 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1014 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 323 CCAGG ^CC[AT]GG EcoRV 600 GATATC GAT^ATC FbaI 357 TGATCA T^GATCA HindIII 347 AAGCTT A^AGCTT HphI 1096 TCACC GGTGA(8/7) MaeI 1141 CTAG C^TAG MaeII 449 ACGT A^CGT MslI 748 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 323 CCAGG CC^[AT]GG NlaIV 780 GGAGCC GG[ACGT]^[ACGT]CC PpuMI 1014 AGGACCT [GA]G^G[AT]CC[CT] PshBI 339 ATTAAT AT^TAAT ScrFI 323 CCAGG CC^[ACGT]GG SmlI 461 CTCAAG C^T[CT][GA]AG SspI 1168 AATATT AAT^ATT TaqI 1112 TCGA T^CGA TthHB8I 1112 TCGA T^CGA XbaI 1140 TCTAGA T^CTAGA XcmI 1233 CCATGTCCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 264 GTATAC GT^[AC][GT]AC 851 GTATAC BsiYI 323 CCAGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1006 CCTTCAGTAGG BslI 323 CCAGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1006 CCTTCAGTAGG Bsp1407I 1080 TGTACA T^GTACA 1201 TGTACA BsrGI 1080 TGTACA T^GTACA 1201 TGTACA Bst1107I 264 GTATAC GTA^TAC 851 GTATAC BstZ17I 264 GTATAC GTA^TAC 851 GTATAC EcoRI 476 GAATTC G^AATTC 1154 GAATTC HaeIII 114 GGCC GG^CC 1246 GGCC NspI 886 GCATGT [GA]CATG^[CT] 1075 GCATGT PstI 704 CTGCAG CTGCA^G 972 CTGCAG SspBI 1080 TGTACA T^GTACA 1201 TGTACA Tsp45I 406 GTGAC ^GT[GC]AC 823 GTCAC TspRI 518 CACTG CA[GC]TG[ACGT][ACGT]^ 944 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BpuAI BsaJI BsmI BstF5I BstYI Cfr13I DdeI DpnII EarI EcoT14I Fnu4HI FokI HinfI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MwoI NdeII NlaIII PleI RsaI Sau3AI Sau96I SfcI StyI TfiI Tru9I TseI Tsp509I XhoII