(Linear) (Six-base) MAPSORT of: KIEE5530.seq Check: 895 from: 1 to: 1290 KIEE5530.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AccI GT'mk_AC Cuts at: 0 265 852 1290 Size: 265 587 438 Fragments arranged by size: 587 438 265 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 621 992 1290 Size: 94 527 371 298 Fragments arranged by size: 527 371 298 94 AlwNI CAG_nnn'CTG Cuts at: 0 10 1290 Size: 10 1280 ApoI r'AATT_y Cuts at: 0 223 388 476 724 1154 1290 Size: 223 165 88 248 430 136 Fragments arranged by size: 430 248 223 165 136 88 AseI AT'TA_AT Cuts at: 0 340 1290 Size: 340 950 BbsI GAAGACnn'nnnn_ Cuts at: 0 82 340 989 1290 Size: 82 258 649 301 Fragments arranged by size: 649 301 258 82 BclI T'GATC_A Cuts at: 0 357 1290 Size: 357 933 BglII A'GATC_T Cuts at: 0 1188 1290 Size: 1188 102 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 804 1290 Size: 804 486 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1290 Size: 446 844 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 833 1290 Size: 833 457 BseYI C'CCAG_C Cuts at: 0 244 1290 Size: 244 1046 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 863 1290 Size: 863 427 BsmI GAATG_Cn' Cuts at: 0 90 705 954 1290 Size: 90 615 249 336 Fragments arranged by size: 615 336 249 90 BsrDI GCAATG_nn' Cuts at: 0 204 1290 Size: 204 1086 BsrGI T'GTAC_A Cuts at: 0 1080 1201 1290 Size: 1080 121 89 Fragments arranged by size: 1080 121 89 BstXI CCAn_nnnn'nTGG Cuts at: 0 669 1290 Size: 669 621 BstYI r'GATC_y Cuts at: 0 657 870 1188 1290 Size: 657 213 318 102 Fragments arranged by size: 657 318 213 102 BstZ17I GTA'TAC Cuts at: 0 266 853 1290 Size: 266 587 437 Fragments arranged by size: 587 437 266 DraI TTT'AAA Cuts at: 0 429 1290 Size: 429 861 EarI CTCTTCn'nnn_ Cuts at: 0 370 640 1037 1290 Size: 370 270 397 253 Fragments arranged by size: 397 370 270 253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 621 992 1290 Size: 94 527 371 298 Fragments arranged by size: 527 371 298 94 EcoNI CCTnn'n_nnAGG Cuts at: 0 1010 1290 Size: 1010 280 EcoO109I rG'GnC_Cy Cuts at: 0 1015 1290 Size: 1015 275 EcoRI G'AATT_C Cuts at: 0 476 1154 1290 Size: 476 678 136 Fragments arranged by size: 678 476 136 EcoRV GAT'ATC Cuts at: 0 602 1290 Size: 602 688 HindIII A'AGCT_T Cuts at: 0 347 1290 Size: 347 943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 889 1290 Size: 889 401 MslI CAynn'nnrTG Cuts at: 0 752 1290 Size: 752 538 NspI r_CATG'y Cuts at: 0 890 1079 1290 Size: 890 189 211 Fragments arranged by size: 890 211 189 PpuMI rG'GwC_Cy Cuts at: 0 1015 1290 Size: 1015 275 PstI C_TGCA'G Cuts at: 0 708 976 1290 Size: 708 268 314 Fragments arranged by size: 708 314 268 SfcI C'TryA_G Cuts at: 0 704 941 972 1290 Size: 704 237 31 318 Fragments arranged by size: 704 318 237 31 SmlI C'TyrA_G Cuts at: 0 461 1290 Size: 461 829 SspI AAT'ATT Cuts at: 0 1170 1290 Size: 1170 120 StyI C'CwwG_G Cuts at: 0 110 662 1036 1290 Size: 110 552 374 254 Fragments arranged by size: 552 374 254 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 916 1290 Size: 916 374 TatI w'GTAC_w Cuts at: 0 996 1080 1201 1290 Size: 996 84 121 89 Fragments arranged by size: 996 121 89 84 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 701 733 1290 Size: 701 32 557 Fragments arranged by size: 701 557 32 XbaI T'CTAG_A Cuts at: 0 1140 1290 Size: 1140 150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1240 1290 Size: 1240 50 Enzymes that do cut: AccI AcuI AlwNI ApoI AseI BbsI BclI BglII BplI BpuEI BseRI BseYI BsgI BsmI BsrDI BsrGI BstXI BstYI BstZ17I DraI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HindIII MmeI MslI NspI PpuMI PstI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI XmnI ZraI