################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AlwNI AscI AseI AsnI Asp718I AvaII AviII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DrdI EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI KpnI MamI MfeI MroI MunI NaeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 473 ACGCGT A^C[GA][CT]GT AhdI 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 66 GTGCAC G^TGCAC AocI 13 CCTCAGG CC^T[ACGT]AGG Aor51HI 1823 AGCGCT AGC^GCT ApaLI 66 GTGCAC G^TGCAC Asp700I 695 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 748 CCTAGG C^CTAGG BbsI 1674 GAAGAC GAAGAC(2/6) BglII 554 AGATCT A^GATCT BlnI 748 CCTAGG C^CTAGG BlpI 1582 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1582 GCTCAGC GC^T[ACGT]AGC BpuAI 1674 GAAGAC GAAGAC(2/6) BsmAI 978 GAGAC GTCTC(1/5) BsmI 1029 GCATTC GAATGC(1/-1) Bsp1407I 1683 TGTACA T^GTACA BsrFI 405 GCCGGT [GA]^CCGG[CT] BsrGI 1683 TGTACA T^GTACA BssSI 63 CTCGTG CACGAG(-5/-1) BstYI 554 AGATCT [GA]^GATC[CT] Bsu36I 13 CCTCAGG CC^T[ACGT]AGG CelII 1582 GCTCAGC GC^T[ACGT]AGC Cfr10I 405 GCCGGT [GA]^CCGG[CT] DraIII 1925 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1330 GACAATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 755 GAAGAG CTCTTC(1/4) Eco47III 1823 AGCGCT AGC^GCT Eco81I 13 CCTCAGG CC^T[ACGT]AGG HindIII 1917 AAGCTT A^AGCTT HpaI 1600 GTTAAC GTT^AAC Ksp632I 755 GAAGAG CTCTTC(1/4) MaeII 1484 ACGT A^CGT MflI 554 AGATCT [GA]^GATC[CT] MluI 473 ACGCGT A^CGCGT NcoI 767 CCATGG C^CATGG NdeI 1274 CATATG CA^TATG PflFI 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 721 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1595 CTGCAG CTGCA^G PvuII 1746 CAGCTG CAG^CTG SacI 522 GAGCTC GAGCT^C SapI 755 GAAGAGC GCTCTTC(1/4) ScaI 964 AGTACT AGT^ACT SfcI 1595 CTGCAG C^T[GA][CT]AG SfiI 291 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 404 CGCCGGTG C[GA]^CCGG[CT]G SmaI 382 CCCGGG CCC^GGG SspBI 1683 TGTACA T^GTACA TspRI 1929 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1170 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 721 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 554 AGATCT [GA]^GATC[CT] XmaCI 382 CCCGGG C^CCGGG XmaI 382 CCCGGG C^CCGGG XmnI 695 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 1441 GATCC GGATC(4/5) 865 GGATC ApaI 333 GGGCCC GGGCC^C 1494 GGGCCC AspHI 66 GTGCAC G[AT]GC[AT]^C 522 GAGCTC AvaI 382 CCCGGG C^[CT]CG[GA]G 1647 CTCGGG BalI 837 TGGCCA TGG^CCA 1278 TGGCCA BglI 292 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 378 GCCGCCCGGGC BsgI 352 CTGCAC GTGCAG(16/14) 676 GTGCAG BsiHKAI 66 GTGCAC G[AT]GC[AT]^C 522 GAGCTC BsoBI 382 CCCGGG C^[CT]CG[GA]G 1647 CTCGGG BspHI 209 TCATGA T^CATGA 1487 TCATGA BsrBI 430 CCGCTC CCGCTC(-3/-3) 735 GAGCGG BsrDI 908 GCAATG GCAATG(2/0) 1751 GCAATG DraII 332 AGGGCCC [GA]G^G[ACGT]CC[CT] 1494 GGGCCCT EcoO109I 332 AGGGCCC [GA]G^G[ACGT]CC[CT] 1494 GGGCCCT EcoRI 630 GAATTC G^AATTC 1242 GAATTC HincII 1512 GTCAAC GT[CT]^[GA]AC 1600 GTTAAC HindII 1512 GTCAAC GT[CT]^[GA]AC 1600 GTTAAC KspI 161 CCGCGG CCGC^GG 296 CCGCGG MluNI 837 TGGCCA TGG^CCA 1278 TGGCCA MscI 837 TGGCCA TGG^CCA 1278 TGGCCA NotI 289 GCGGCCGC GC^GGCCGC 298 GCGGCCGC NspI 1578 ACATGC [GA]CATG^[CT] 1811 GCATGT RcaI 209 TCATGA T^CATGA 1487 TCATGA SacII 161 CCGCGG CCGC^GG 296 CCGCGG TfiI 1551 GAATC G^A[AT]TC 1843 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI ApoI BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI StyI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I XcmI