(Circular) (Six-base) MAPSORT of: pFN21AB7061.seq Check: 3549 from: 1 to: 6670 pFN21AB7061 6670 bp With 182 enzymes: SgfI * February 18, 2014 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3314 4733 6117 6385 1632 Size: 1682 1419 1384 268 1917 Fragments arranged by size: 1917 1682 1419 1384 268 AfeI AGC'GCT Cuts at: 3830 3830 Size: 6670 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 2478 5569 6546 1203 Size: 1275 3091 977 1327 Fragments arranged by size: 3091 1327 1275 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 3340 5399 3340 Size: 2059 4611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3215 3615 3738 1184 Size: 2031 400 123 4116 Fragments arranged by size: 4116 2031 400 123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3803 3835 3803 Size: 32 6638 AlwNI CAG_nnn'CTG Cuts at: 4413 5985 4413 Size: 1572 5098 ApaI G_GGCC'C Cuts at: 2342 3503 2342 Size: 1161 5509 ApaLI G'TGCA_C Cuts at: 2071 4727 5883 2071 Size: 2656 1156 2858 Fragments arranged by size: 2858 2656 1156 ApoI r'AATT_y Cuts at: 1493 2635 2789 3247 3979 4205 4357 6466 1493 Size: 1142 154 458 732 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 732 458 226 154 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2387 3652 3995 1870 Size: 73 444 1265 343 4545 Fragments arranged by size: 4545 1265 444 343 73 AvrII C'CTAG_G Cuts at: 2753 2753 Size: 6670 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2131 2260 2413 3191 Size: 325 217 627 227 117 129 153 778 Cuts at: 3191 3991 5446 618 Size: 800 1455 1842 Fragments arranged by size: 1842 1455 800 778 627 325 227 217 153 129 117 BanII G_rGCy'C Cuts at: 1391 2342 2531 3302 3503 3989 1391 Size: 951 189 771 201 486 4072 Fragments arranged by size: 4072 951 771 486 201 189 BbeI G_GCGC'C Cuts at: 2018 2135 2264 2018 Size: 117 129 6424 Fragments arranged by size: 6424 129 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3686 928 Size: 971 1787 3912 Fragments arranged by size: 3912 1787 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 2389 5281 Size: 107 122 71 631 605 631 86 2892 Cuts at: 5281 136 Size: 1525 Fragments arranged by size: 2892 1525 631 631 605 122 107 86 71 BglII A'GATC_T Cuts at: 2559 6665 2559 Size: 4106 2564 BlpI GC'TnA_GC Cuts at: 3588 4087 3588 Size: 499 6171 Bme1580I G_kGCm'C Cuts at: 1790 2075 2342 2418 2589 3503 3727 4731 5887 Size: 285 267 76 171 914 224 1004 1156 Cuts at: 5887 1790 Size: 2573 Fragments arranged by size: 2573 1156 1004 914 285 267 224 171 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 3172 4401 Size: 416 53 83 399 24 1661 94 1229 Cuts at: 4401 5359 442 Size: 958 1753 Fragments arranged by size: 1753 1661 1229 958 416 399 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2107 2255 2819 2953 5330 1239 Size: 868 148 564 134 2377 2579 Fragments arranged by size: 2579 2377 868 564 148 134 Bpu10I CC'TnA_GC Cuts at: 2225 2225 Size: 6670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4795 5660 5958 4795 Size: 865 298 5507 Fragments arranged by size: 5507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2132 2163 2261 Size: 53 83 186 936 482 117 31 98 Cuts at: 2261 3209 3613 4477 4859 5494 275 Size: 948 404 864 382 635 1451 Fragments arranged by size: 1451 948 936 864 635 482 404 382 186 117 98 83 53 31 BsaWI w'CCGG_w Cuts at: 5103 5501 5775 5922 6333 5103 Size: 398 274 147 411 5440 Fragments arranged by size: 5440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6445 Fragments arranged by size: 6445 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 3405 3730 1186 Size: 1066 1153 325 4126 Fragments arranged by size: 4126 1153 1066 325 BseYI C'CCAG_C Cuts at: 2362 2429 2855 3254 3459 3560 3957 5873 6369 Size: 67 426 399 205 101 397 1916 496 Cuts at: 6369 2362 Size: 2663 Fragments arranged by size: 2663 1916 496 426 399 397 205 101 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2702 1163 Size: 573 606 360 5131 Fragments arranged by size: 5131 606 573 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2063 2298 2307 4120 4881 5909 Size: 275 186 62 235 9 1813 761 1028 Cuts at: 5909 1540 Size: 2301 Fragments arranged by size: 2301 1813 1028 761 275 235 186 62 9 BsiHKAI G_wGCw'C Cuts at: 2075 2531 4731 4816 5887 2075 Size: 456 2200 85 1071 2858 Fragments arranged by size: 2858 2200 1071 456 85 BsmI GAATG_Cn' Cuts at: 3034 4181 4274 3034 Size: 1147 93 5430 Fragments arranged by size: 5430 1147 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2342 2418 2531 2589 3302 3503 Size: 399 285 267 76 113 58 713 201 Cuts at: 3503 3727 3989 4731 4816 5887 1391 Size: 224 262 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 399 285 267 262 224 201 113 85 76 58 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2214 3492 4559 6289 2214 Size: 1278 1067 1730 2595 Fragments arranged by size: 2595 1730 1278 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4025 844 Size: 3181 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2437 2742 4557 1394 Size: 429 614 305 1815 3507 Fragments arranged by size: 3507 1815 614 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 2920 3763 5165 5339 65 Size: 1183 1672 843 1402 174 1396 Fragments arranged by size: 1672 1402 1396 1183 843 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2410 5314 5501 1700 Size: 110 600 2904 187 2869 Fragments arranged by size: 2904 2869 600 187 110 BsrGI T'GTAC_A Cuts at: 96 3688 96 Size: 3592 3078 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 2068 4730 5742 2068 Size: 2662 1012 2996 Fragments arranged by size: 2996 2662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6670 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstYI r'GATC_y Cuts at: 1972 2559 4000 4367 4752 4769 6221 6307 6319 Size: 587 1441 367 385 17 1452 86 12 Cuts at: 6319 6665 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1441 587 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2019 2019 Size: 6670 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 2271 2301 2772 513 Size: 552 939 162 105 30 471 4411 Fragments arranged by size: 4411 939 552 471 162 105 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5931 Fragments arranged by size: 5931 683 56 BtsI GCAGTG_nn' Cuts at: 4186 4980 5000 4186 Size: 794 20 5856 Fragments arranged by size: 5856 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 3935 3935 Size: 6670 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2060 2078 Size: 54 1425 50 275 18 177 53 18 Cuts at: 2078 2115 2295 2304 2842 3283 4117 5006 6635 Size: 37 180 9 538 441 834 889 1629 Cuts at: 6635 8 Size: 43 Fragments arranged by size: 1629 1425 889 834 538 441 275 180 177 54 53 50 43 37 18 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2060 2295 2304 4117 1537 Size: 275 248 235 9 1813 4090 Fragments arranged by size: 4090 1813 275 248 235 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2755 4600 1353 Size: 1402 1845 3423 Fragments arranged by size: 3423 1845 1402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2549 5257 5631 5777 387 Size: 1064 138 960 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 960 374 146 138 EcoICRI GAG'CTC Cuts at: 2529 2529 Size: 6670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2255 2819 2953 3314 4733 5330 Size: 393 475 148 564 134 361 1419 597 Cuts at: 5330 6117 6385 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1419 787 597 564 475 393 361 268 148 134 EcoO109I rG'GnC_Cy Cuts at: 2338 3500 2338 Size: 1162 5508 EcoRI G'AATT_C Cuts at: 2635 3247 3979 4357 2635 Size: 612 732 378 4948 Fragments arranged by size: 4948 732 612 378 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 2018 2135 2208 2264 2369 3832 5817 1678 Size: 340 117 73 56 105 1463 1985 2531 Fragments arranged by size: 2531 1985 1463 340 117 105 73 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2707 2739 3088 3120 3326 Size: 32 135 32 1135 32 349 32 206 Cuts at: 3326 3358 3833 3865 5311 5343 5385 5417 1373 Size: 32 475 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1446 1135 475 349 206 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3607 4014 4260 669 Size: 988 281 1581 88 407 246 3079 Fragments arranged by size: 3079 1581 988 407 281 246 88 HindIII A'AGCT_T Cuts at: 748 3922 748 Size: 3174 3496 HpaI GTT'AAC Cuts at: 3607 4260 3607 Size: 653 6017 KasI G'GCGC_C Cuts at: 2014 2131 2260 2014 Size: 117 129 6424 Fragments arranged by size: 6424 129 117 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 2478 6546 2478 Size: 4068 2602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 5784 5968 1305 Size: 828 3651 184 2007 Fragments arranged by size: 3651 2007 828 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2844 3285 10 Size: 54 1425 343 1012 441 3395 Fragments arranged by size: 3395 1425 1012 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 2913 3197 3716 4628 4987 5146 Size: 648 1512 235 284 519 912 359 159 Cuts at: 5146 518 Size: 2042 Fragments arranged by size: 2042 1512 912 648 519 359 284 235 159 MspA1I CmG'CkG Cuts at: 1606 2053 2083 2168 2198 2303 2395 2429 3753 Size: 447 30 85 30 105 92 34 1324 Cuts at: 3753 4086 4763 5911 6156 1606 Size: 333 677 1148 245 2120 Fragments arranged by size: 2120 1324 1148 677 447 333 245 105 92 85 34 30 30 NarI GG'CG_CC Cuts at: 2015 2132 2261 2015 Size: 117 129 6424 Fragments arranged by size: 6424 129 117 NcoI C'CATG_G Cuts at: 513 1065 2004 2772 513 Size: 552 939 768 4411 Fragments arranged by size: 4411 939 768 552 NdeI CA'TA_TG Cuts at: 387 3280 387 Size: 2893 3777 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 2295 2304 4117 2295 Size: 9 1813 4848 Fragments arranged by size: 4848 1813 9 NspI r_CATG'y Cuts at: 1132 1229 3587 3820 4028 5573 1132 Size: 97 2358 233 208 1545 2229 Fragments arranged by size: 2358 2229 1545 233 208 97 PasI CC'CwG_GG Cuts at: 1348 3727 1348 Size: 2379 4291 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2732 1108 Size: 1624 5046 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 2338 3499 2338 Size: 1161 5509 PstI C_TGCA'G Cuts at: 830 3604 4022 830 Size: 2774 418 3478 Fragments arranged by size: 3478 2774 418 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 3753 3753 Size: 6670 SacI G_AGCT'C Cuts at: 2531 2531 Size: 6670 SacII CC_GC'GG Cuts at: 2169 2304 2169 Size: 135 6535 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SapI GCTCTTCn'nnn_ Cuts at: 2755 2755 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 2971 4918 6630 2971 Size: 1947 1712 3011 Fragments arranged by size: 3011 1947 1712 SfcI C'TryA_G Cuts at: 826 1045 3600 4018 5153 5834 6025 826 Size: 219 2555 418 1135 681 191 1471 Fragments arranged by size: 2555 1471 1135 681 418 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 6670 SfoI GGC'GCC Cuts at: 2016 2133 2262 2016 Size: 117 129 6424 Fragments arranged by size: 6424 129 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 2410 1700 Size: 710 5960 SmaI CCC'GGG Cuts at: 1872 2389 3997 1872 Size: 517 1608 4545 Fragments arranged by size: 4545 1608 517 SmlI C'TyrA_G Cuts at: 820 1017 1943 4774 5675 5937 820 Size: 197 926 2831 901 262 1553 Fragments arranged by size: 2831 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SrfI GCCC'GGGC Cuts at: 2389 2389 Size: 6670 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6670 StyI C'CwwG_G Cuts at: 513 1065 2004 2514 2753 2772 4109 513 Size: 552 939 510 239 19 1337 3074 Fragments arranged by size: 3074 1337 939 552 510 239 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 3007 3017 3268 3499 3949 4697 Size: 1255 796 631 10 251 231 450 748 Cuts at: 4697 4714 4867 5052 5497 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 796 748 631 450 445 251 231 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2936 2969 3688 Size: 274 80 33 51 1443 959 33 719 Cuts at: 3688 4916 6628 96 Size: 1228 1712 138 Fragments arranged by size: 1712 1443 1228 959 719 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6630 6662 6630 Size: 32 6638 Tth111I GACn'n_nGTC Cuts at: 1316 3178 1316 Size: 1862 4808 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2852 3185 3575 2852 Size: 333 390 5947 Fragments arranged by size: 5947 390 333 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 2387 3995 1870 Size: 517 1608 4545 Fragments arranged by size: 4545 1608 517 XmnI GAAnn'nnTTC Cuts at: 2704 4799 2704 Size: 2095 4575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SwaI