(Linear) (Six-base) MAPSORT of: KIBB7061.seq Check: 1862 from: 1 to: 1965 KIBB7061.orf With 182 enzymes: SgfI * February 18, 2014 13:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1309 1965 Size: 1309 656 AfeI AGC'GCT Cuts at: 0 1825 1965 Size: 1825 140 AflIII A'CryG_T Cuts at: 0 473 1965 Size: 473 1492 AhdI GACnn_n'nnGTC Cuts at: 0 1335 1965 Size: 1335 630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1210 1610 1733 1965 Size: 1210 400 123 232 Fragments arranged by size: 1210 400 232 123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1798 1830 1965 Size: 1798 32 135 Fragments arranged by size: 1798 135 32 ApaI G_GGCC'C Cuts at: 0 337 1498 1965 Size: 337 1161 467 Fragments arranged by size: 1161 467 337 ApaLI G'TGCA_C Cuts at: 0 66 1965 Size: 66 1899 ApoI r'AATT_y Cuts at: 0 630 784 1242 1965 Size: 630 154 458 723 Fragments arranged by size: 723 630 458 154 AvaI C'yCGr_G Cuts at: 0 382 1647 1965 Size: 382 1265 318 Fragments arranged by size: 1265 382 318 AvrII C'CTAG_G Cuts at: 0 748 1965 Size: 748 1217 BanI G'GyrC_C Cuts at: 0 9 126 255 408 1186 1965 Size: 9 117 129 153 778 779 Fragments arranged by size: 779 778 153 129 117 9 BanII G_rGCy'C Cuts at: 0 337 526 1297 1498 1965 Size: 337 189 771 201 467 Fragments arranged by size: 771 467 337 201 189 BbeI G_GCGC'C Cuts at: 0 13 130 259 1965 Size: 13 117 129 1706 Fragments arranged by size: 1706 129 117 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 1681 1965 Size: 1681 284 BglI GCCn_nnn'nGGC Cuts at: 0 298 384 1965 Size: 298 86 1581 Fragments arranged by size: 1581 298 86 BglII A'GATC_T Cuts at: 0 554 1965 Size: 554 1411 BlpI GC'TnA_GC Cuts at: 0 1583 1965 Size: 1583 382 Bme1580I G_kGCm'C Cuts at: 0 70 337 413 584 1498 1722 1965 Size: 70 267 76 171 914 224 243 Fragments arranged by size: 914 267 243 224 171 76 70 BmrI ACTGGGnnnn_n' Cuts at: 0 1073 1167 1965 Size: 1073 94 798 Fragments arranged by size: 1073 798 94 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 250 814 948 1965 Size: 102 148 564 134 1017 Fragments arranged by size: 1017 564 148 134 102 Bpu10I CC'TnA_GC Cuts at: 0 220 1965 Size: 220 1745 BsaHI Gr'CG_yC Cuts at: 0 10 127 158 256 1204 1608 1965 Size: 10 117 31 98 948 404 357 Fragments arranged by size: 948 404 357 117 98 31 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 247 1400 1725 1965 Size: 247 1153 325 240 Fragments arranged by size: 1153 325 247 240 BseYI C'CCAG_C Cuts at: 0 357 424 850 1249 1454 1555 1952 1965 Size: 357 67 426 399 205 101 397 13 Fragments arranged by size: 426 399 397 357 205 101 67 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 697 1965 Size: 337 360 1268 Fragments arranged by size: 1268 360 337 BsiEI CG_ry'CG Cuts at: 0 58 293 302 1965 Size: 58 235 9 1663 Fragments arranged by size: 1663 235 58 9 BsiHKAI G_wGCw'C Cuts at: 0 70 526 1965 Size: 70 456 1439 Fragments arranged by size: 1439 456 70 BsmI GAATG_Cn' Cuts at: 0 1029 1965 Size: 1029 936 Bsp1286I G_dGCh'C Cuts at: 0 70 337 413 526 584 1297 1498 1722 Size: 70 267 76 113 58 713 201 224 Cuts at: 1722 1965 Size: 243 Fragments arranged by size: 713 267 243 224 201 113 76 70 58 BspHI T'CATG_A Cuts at: 0 209 1487 1965 Size: 209 1278 478 Fragments arranged by size: 1278 478 209 BsrBI CCG'CTC Cuts at: 0 432 737 1965 Size: 432 305 1228 Fragments arranged by size: 1228 432 305 BsrDI GCAATG_nn' Cuts at: 0 915 1758 1965 Size: 915 843 207 Fragments arranged by size: 915 843 207 BsrFI r'CCGG_y Cuts at: 0 405 1965 Size: 405 1560 BsrGI T'GTAC_A Cuts at: 0 1683 1965 Size: 1683 282 BssSI C'ACGA_G Cuts at: 0 63 1965 Size: 63 1902 BstYI r'GATC_y Cuts at: 0 554 1965 Size: 554 1411 Bsu36I CC'TnA_GG Cuts at: 0 14 1965 Size: 14 1951 BtgI C'CryG_G Cuts at: 0 161 266 296 767 1965 Size: 161 105 30 471 1198 Fragments arranged by size: 1198 471 161 105 30 DraIII CAC_nnn'GTG Cuts at: 0 1930 1965 Size: 1930 35 EaeI y'GGCC_r Cuts at: 0 2 55 73 110 290 299 837 1278 Size: 2 53 18 37 180 9 538 441 Cuts at: 1278 1965 Size: 687 Fragments arranged by size: 687 538 441 180 53 37 18 9 2 EagI C'GGCC_G Cuts at: 0 55 290 299 1965 Size: 55 235 9 1666 Fragments arranged by size: 1666 235 55 9 EarI CTCTTCn'nnn_ Cuts at: 0 750 1965 Size: 750 1215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 544 1965 Size: 544 1421 EcoICRI GAG'CTC Cuts at: 0 524 1965 Size: 524 1441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 250 814 948 1309 1965 Size: 102 148 564 134 361 656 Fragments arranged by size: 656 564 361 148 134 102 EcoO109I rG'GnC_Cy Cuts at: 0 333 1495 1965 Size: 333 1162 470 Fragments arranged by size: 1162 470 333 EcoRI G'AATT_C Cuts at: 0 630 1242 1965 Size: 630 612 723 Fragments arranged by size: 723 630 612 HaeII r_GCGC'y Cuts at: 0 13 130 203 259 364 1827 1965 Size: 13 117 73 56 105 1463 138 Fragments arranged by size: 1463 138 117 105 73 56 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 702 734 1083 1115 1321 1353 1828 1860 Size: 702 32 349 32 206 32 475 32 Cuts at: 1860 1965 Size: 105 Fragments arranged by size: 702 475 349 206 105 32 32 32 32 HincII GTy'rAC Cuts at: 0 1514 1602 1965 Size: 1514 88 363 Fragments arranged by size: 1514 363 88 HindIII A'AGCT_T Cuts at: 0 1917 1965 Size: 1917 48 HpaI GTT'AAC Cuts at: 0 1602 1965 Size: 1602 363 KasI G'GCGC_C Cuts at: 0 9 126 255 1965 Size: 9 117 129 1710 Fragments arranged by size: 1710 129 117 9 MluI A'CGCG_T Cuts at: 0 473 1965 Size: 473 1492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 128 1965 Size: 128 1837 MscI TGG'CCA Cuts at: 0 839 1280 1965 Size: 839 441 685 Fragments arranged by size: 839 685 441 MslI CAynn'nnrTG Cuts at: 0 673 908 1192 1711 1965 Size: 673 235 284 519 254 Fragments arranged by size: 673 519 284 254 235 MspA1I CmG'CkG Cuts at: 0 48 78 163 193 298 390 424 1748 Size: 48 30 85 30 105 92 34 1324 Cuts at: 1748 1965 Size: 217 Fragments arranged by size: 1324 217 105 92 85 48 34 30 30 NarI GG'CG_CC Cuts at: 0 10 127 256 1965 Size: 10 117 129 1709 Fragments arranged by size: 1709 129 117 10 NcoI C'CATG_G Cuts at: 0 767 1965 Size: 767 1198 NdeI CA'TA_TG Cuts at: 0 1275 1965 Size: 1275 690 NotI GC'GGCC_GC Cuts at: 0 290 299 1965 Size: 290 9 1666 Fragments arranged by size: 1666 290 9 NspI r_CATG'y Cuts at: 0 1582 1815 1965 Size: 1582 233 150 Fragments arranged by size: 1582 233 150 PasI CC'CwG_GG Cuts at: 0 1722 1965 Size: 1722 243 PflMI CCAn_nnn'nTGG Cuts at: 0 727 1965 Size: 727 1238 PspOMI G'GGCC_C Cuts at: 0 333 1494 1965 Size: 333 1161 471 Fragments arranged by size: 1161 471 333 PstI C_TGCA'G Cuts at: 0 1599 1965 Size: 1599 366 PvuII CAG'CTG Cuts at: 0 1748 1965 Size: 1748 217 SacI G_AGCT'C Cuts at: 0 526 1965 Size: 526 1439 SacII CC_GC'GG Cuts at: 0 164 299 1965 Size: 164 135 1666 Fragments arranged by size: 1666 164 135 SapI GCTCTTCn'nnn_ Cuts at: 0 750 1965 Size: 750 1215 ScaI AGT'ACT Cuts at: 0 966 1965 Size: 966 999 SfcI C'TryA_G Cuts at: 0 1595 1965 Size: 1595 370 SfiI GGCCn_nnn'nGGCC Cuts at: 0 298 1965 Size: 298 1667 SfoI GGC'GCC Cuts at: 0 11 128 257 1965 Size: 11 117 129 1708 Fragments arranged by size: 1708 129 117 11 SgrAI Cr'CCGG_yG Cuts at: 0 405 1965 Size: 405 1560 SmaI CCC'GGG Cuts at: 0 384 1965 Size: 384 1581 SrfI GCCC'GGGC Cuts at: 0 384 1965 Size: 384 1581 StyI C'CwwG_G Cuts at: 0 509 748 767 1965 Size: 509 239 19 1198 Fragments arranged by size: 1198 509 239 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 371 1002 1012 1263 1494 1944 1965 Size: 371 631 10 251 231 450 21 Fragments arranged by size: 631 450 371 251 231 21 10 TatI w'GTAC_w Cuts at: 0 931 964 1683 1965 Size: 931 33 719 282 Fragments arranged by size: 931 719 282 33 Tth111I GACn'n_nGTC Cuts at: 0 1173 1965 Size: 1173 792 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 847 1180 1570 1965 Size: 847 333 390 395 Fragments arranged by size: 847 395 390 333 XmaI C'CCGG_G Cuts at: 0 382 1965 Size: 382 1583 XmnI GAAnn'nnTTC Cuts at: 0 699 1965 Size: 699 1266 Enzymes that do cut: AcuI AfeI AflIII AhdI AlfI AloI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstYI Bsu36I BtgI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII HpaI KasI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NotI NspI PasI PflMI PspOMI PstI PvuII SacI SacII SapI ScaI SfcI SfiI SfoI SgrAI SmaI SrfI StyI TaqII TatI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AleI AlwNI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspMI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EcoNI EcoRV FalI FseI FspI FspAI KpnI MfeI NaeI NgoMIV NheI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SexAI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI TstI XbaI XhoI ZraI