################################## # NO CUT ENZYMES ( 218 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MflI MluI MluNI MnlI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AatII 30 GACGTC GACGT^C AciI 222 GCGG CCGC(-3/-1) AcsI 115 GAATTT [GA]^AATT[CT] AcyI 30 GACGTC G[GA]^CG[CT]C ApoI 115 GAATTT [GA]^AATT[CT] AvaII 36 GGACC G^G[AT]CC BfaI 233 CTAG C^TAG BsaHI 30 GACGTC G[GA]^CG[CT]C BsaJI 40 CCACGG C^C[ACGT][ACGT]GG BsgI 176 CTGCAC GTGCAG(16/14) Cfr13I 36 GGACC G^G[ACGT]CC DdeI 61 CTCAG C^T[ACGT]AG DraI 118 TTTAAA TTT^AAA DsaI 40 CCACGG C^C[GA][CT]GG Hin1I 30 GACGTC G[GA]^CG[CT]C MaeI 233 CTAG C^TAG MaeII 31 ACGT A^CGT MboII 112 GAAGA GAAGA(8/7) NlaIII 208 CATG CATG^ NlaIV 36 GGACCC GG[ACGT]^[ACGT]CC Sau96I 36 GGACC G^G[ACGT]CC ScaI 57 AGTACT AGT^ACT TaqI 4 TCGA T^CGA TspRI 63 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 4 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BstAPI 133 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 178 GCACAGACTGC DpnII 21 GATC ^GATC 238 GATC MboI 21 GATC ^GATC 238 GATC MwoI 133 GCAACAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 178 GCACAGACTGC NdeII 21 GATC ^GATC 238 GATC Sau3AI 21 GATC ^GATC 238 GATC Tsp509I 116 AATT ^AATT 144 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AfaI BbvI Fnu4HI ItaI MseI RsaI Tru9I TseI