################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI FauI FseI FspI HapII HindIII HpaII KasI KspI MamI MluI MluNI MroI MscI MspI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 700 GGTACC G^GTACC AccII 1465 CGCG CG^CG AciI 1464 CCGC CCGC(-3/-1) AcyI 352 GACGCC G[GA]^CG[CT]C AlwNI 2096 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 700 GGTACC G^GTACC BamHI 388 GGATCC G^GATCC BanII 1567 GAGCTC G[GA]GC[CT]^C BbvCI 1536 CCTCAGC CCTCAGC(-5/-2) BglII 80 AGATCT A^GATCT BsaHI 352 GACGCC G[GA]^CG[CT]C BsmI 486 GAATGC GAATGC(1/-1) BspHI 1841 TCATGA T^CATGA BstUI 1465 CGCG CG^CG BstXI 297 CCATGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1752 TTTAAA TTT^AAA DraIII 848 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 636 CCATGG C^C[GA][CT]GG EcoRV 442 GATATC GAT^ATC Hin1I 352 GACGCC G[GA]^CG[CT]C HpaI 46 GTTAAC GTT^AAC KpnI 700 GGTACC GGTAC^C MaeII 467 ACGT A^CGT MfeI 983 CAATTG C^AATTG MunI 983 CAATTG C^AATTG MvnI 1465 CGCG CG^CG NcoI 636 CCATGG C^CATGG RcaI 1841 TCATGA T^CATGA SacI 1567 GAGCTC GAGCT^C SapI 1672 GCTCTTC GCTCTTC(1/4) SexAI 1734 ACCTGGT A^CC[AT]GGT SpeI 1633 ACTAGT A^CTAGT XbaI 2305 TCTAGA T^CTAGA XcmI 591 CCATTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Aor51HI 341 AGCGCT AGC^GCT 1520 AGCGCT AspHI 623 GTGCTC G[AT]GC[AT]^C 1567 GAGCTC BbsI 2309 GAAGAC GAAGAC(2/6) 1375 GTCTTC BclI 1083 TGATCA T^GATCA 1911 TGATCA BpuAI 2309 GAAGAC GAAGAC(2/6) 1375 GTCTTC BsiHKAI 623 GTGCTC G[AT]GC[AT]^C 1567 GAGCTC BsmBI 794 GAGACG CGTCTC(1/5) 964 GAGACG BssSI 848 CACGAG CACGAG(-5/-1) 2075 CACGAG BstEII 2178 GGTCACC G^GT[ACGT]ACC 2187 GGTCACC BstPI 2178 GGTCACC G^GT[ACGT]ACC 2187 GGTCACC Eco47III 341 AGCGCT AGC^GCT 1520 AGCGCT EcoO65I 2178 GGTCACC G^GT[ACGT]ACC 2187 GGTCACC EcoT22I 1232 ATGCAT ATGCA^T 1413 ATGCAT FbaI 1083 TGATCA T^GATCA 1911 TGATCA HgaI 352 GACGC GACGC(5/10) 966 GACGC HincII 46 GTTAAC GT[CT]^[GA]AC 522 GTTGAC HindII 46 GTTAAC GT[CT]^[GA]AC 522 GTTGAC MslI 298 CATGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2262 CACACCCGTG MspA1I 755 CAGCTG C[AC]G^C[GT]G 1391 CAGCTG MwoI 308 GCACTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 886 GCTTCCGAAGC NsiI 1232 ATGCAT ATGCA^T 1413 ATGCAT PleI 937 GAGTC GAGTC(4/5) 1373 GAGTC PpuMI 1088 AGGTCCT [GA]G^G[AT]CC[CT] 2097 AGGTCCT PstI 1285 CTGCAG CTGCA^G 1553 CTGCAG PvuII 755 CAGCTG CAG^CTG 1391 CAGCTG SfcI 1285 CTGCAG C^T[GA][CT]AG 1553 CTGCAG TaqI 377 TCGA T^CGA 627 TCGA TspRI 612 CACTG CA[GC]TG[ACGT][ACGT]^ 928 CAGTG TthHB8I 377 TCGA T^CGA 627 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AseI AsnI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BseRI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV PshBI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I XhoII