(Circular) (Six-base) MAPSORT of: pFN21AE5758.seq Check: 2213 from: 1 to: 7042 pFN21AE5758 7042 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4852 278 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4385 1937 Size: 2448 4594 Acc65I G'GTAC_C Cuts at: 2705 4363 2705 Size: 1658 5384 AclI AA'CG_TT Cuts at: 1563 5169 5542 1563 Size: 3606 373 3063 Fragments arranged by size: 3606 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 4181 4241 5105 6489 6757 1632 Size: 526 2023 60 864 1384 268 1917 Fragments arranged by size: 2023 1917 1384 864 526 268 60 AfeI AGC'GCT Cuts at: 2348 3527 2348 Size: 1179 5863 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6845 AflIII A'CryG_T Cuts at: 1203 5941 6918 1203 Size: 4738 977 1327 Fragments arranged by size: 4738 1327 977 AgeI A'CCGG_T Cuts at: 5873 5873 Size: 7042 AhdI GACnn_n'nnGTC Cuts at: 5771 5771 Size: 7042 AleI CACnn'nnGTG Cuts at: 1166 4271 1166 Size: 3105 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7042 AlwNI CAG_nnn'CTG Cuts at: 4106 4785 6357 4106 Size: 679 1572 4791 Fragments arranged by size: 4791 1572 679 ApaLI G'TGCA_C Cuts at: 5099 6255 5099 Size: 1156 5886 ApoI r'AATT_y Cuts at: 1493 3412 3654 4199 4351 4577 4729 6838 1493 Size: 1919 242 545 152 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 545 242 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 2514 3284 3944 5596 160 Size: 872 1482 770 660 1652 1606 Fragments arranged by size: 1652 1606 1482 872 770 660 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 AvaI C'yCGr_G Cuts at: 1870 1943 4367 1870 Size: 73 2424 4545 Fragments arranged by size: 4545 2424 73 BamHI G'GATC_C Cuts at: 2393 4372 4739 2393 Size: 1979 367 4696 Fragments arranged by size: 4696 1979 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2705 3223 4363 5818 Size: 325 217 627 369 549 518 1140 1455 Cuts at: 5818 618 Size: 1842 Fragments arranged by size: 1842 1455 1140 627 549 518 369 325 217 BanII G_rGCy'C Cuts at: 1391 3576 4361 1391 Size: 2185 785 4072 Fragments arranged by size: 4072 2185 785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 4321 928 Size: 971 1474 948 3649 Fragments arranged by size: 3649 1474 971 948 BbvCI CC'TCA_GC Cuts at: 3542 3542 Size: 7042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5233 5267 1903 Size: 34 3296 34 3678 Fragments arranged by size: 3678 3296 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4934 6144 4934 Size: 1210 5832 BclI T'GATC_A Cuts at: 1548 3088 3916 1548 Size: 1540 828 4674 Fragments arranged by size: 4674 1540 828 BfrBI ATG'CAT Cuts at: 3239 3420 3239 Size: 181 6861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5653 136 Size: 107 122 71 631 605 3981 1525 Fragments arranged by size: 3981 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 7037 2085 Size: 4952 2090 BlpI GC'TnA_GC Cuts at: 4459 4459 Size: 7042 Bme1580I G_kGCm'C Cuts at: 1790 2140 2159 5103 6259 1790 Size: 350 19 2944 1156 2573 Fragments arranged by size: 2944 2573 1156 350 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4773 5731 442 Size: 416 53 83 399 24 3356 958 1753 Fragments arranged by size: 3356 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 3609 4155 5702 1239 Size: 1676 694 546 1547 2579 Fragments arranged by size: 2579 1676 1547 694 546 Bpu10I CC'TnA_GC Cuts at: 3542 3542 Size: 7042 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5167 6032 6330 5167 Size: 865 298 5879 Fragments arranged by size: 5879 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5705 882 Size: 617 4206 2219 Fragments arranged by size: 4206 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4833 493 Size: 711 3629 2702 Fragments arranged by size: 3629 2702 711 BsaBI GATnn'nnATC Cuts at: 7036 7036 Size: 7042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 4849 5231 5866 Size: 53 83 186 936 825 2491 382 635 Cuts at: 5866 275 Size: 1451 Fragments arranged by size: 2491 1451 936 825 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5475 5873 6147 6294 6705 5475 Size: 398 274 147 411 5812 Fragments arranged by size: 5812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436 Size: 30 165 30 1468 30 45 30 460 Cuts at: 3436 3466 4041 4071 1178 Size: 30 575 30 4149 Fragments arranged by size: 4149 1468 575 460 165 45 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2970 3875 1186 Size: 1204 580 905 4353 Fragments arranged by size: 4353 1204 905 580 BseYI C'CCAG_C Cuts at: 2281 6245 6741 2281 Size: 3964 496 2582 Fragments arranged by size: 3964 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6469 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4492 5253 6281 1540 Size: 275 186 2491 761 1028 2301 Fragments arranged by size: 2491 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3576 5103 5188 6259 2632 Size: 944 1527 85 1071 3415 Fragments arranged by size: 3415 1527 1071 944 85 BsmI GAATG_Cn' Cuts at: 2497 4553 4646 2497 Size: 2056 93 4893 Fragments arranged by size: 4893 2056 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2963 2793 Size: 170 6872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2159 2632 3576 4361 5103 5188 Size: 399 350 19 473 944 785 742 85 Cuts at: 5188 6259 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 785 742 473 399 350 85 19 BspEI T'CCGG_A Cuts at: 6705 6705 Size: 7042 BspHI T'CATG_A Cuts at: 3846 4931 6661 3846 Size: 1085 1730 4227 Fragments arranged by size: 4227 1730 1085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4397 844 Size: 3553 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4929 1394 Size: 429 3106 3507 Fragments arranged by size: 3507 3106 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5537 5711 65 Size: 1183 4289 174 1396 Fragments arranged by size: 4289 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5686 5873 1700 Size: 110 3876 187 2869 Fragments arranged by size: 3876 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7042 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7042 BssSI C'ACGA_G Cuts at: 2853 4080 5102 6114 2853 Size: 1227 1022 1012 3781 Fragments arranged by size: 3781 1227 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7042 BstBI TT'CG_AA Cuts at: 5846 5846 Size: 7042 BstEII G'GTnAC_C Cuts at: 1184 4183 4192 1184 Size: 2999 9 4034 Fragments arranged by size: 4034 2999 9 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2309 Size: 7042 BstYI r'GATC_y Cuts at: 1972 2085 2393 3160 4307 4372 4739 5124 5141 Size: 113 308 767 1147 65 367 385 17 Cuts at: 5141 6593 6679 6691 7037 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1147 767 385 367 346 308 113 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 513 Size: 552 1576 4914 Fragments arranged by size: 4914 1576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6303 Fragments arranged by size: 6303 683 56 BtsI GCAGTG_nn' Cuts at: 4558 5352 5372 4558 Size: 794 20 6228 Fragments arranged by size: 6228 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7042 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7007 DraI TTT'AAA Cuts at: 3759 4346 3759 Size: 587 6455 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 7042 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6049 809 Size: 507 4733 1802 Fragments arranged by size: 4733 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4489 5378 7007 Size: 54 1425 50 275 18 2659 889 1629 Cuts at: 7007 8 Size: 43 Fragments arranged by size: 2659 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4489 1537 Size: 275 2677 4090 Fragments arranged by size: 4090 2677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 3684 4173 4972 1353 Size: 1438 893 489 799 3423 Fragments arranged by size: 3423 1438 893 799 489 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5629 6003 6149 387 Size: 1064 138 4040 374 146 1280 Fragments arranged by size: 4040 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 3574 Size: 7042 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2915 3609 4155 4181 4241 5105 Size: 393 526 757 694 546 26 60 864 Cuts at: 5105 5702 6489 6757 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 864 787 757 694 597 546 526 393 268 60 26 EcoO109I rG'GnC_Cy Cuts at: 2788 3094 4103 2788 Size: 306 1009 5727 Fragments arranged by size: 5727 1009 306 EcoRI G'AATT_C Cuts at: 4351 4729 4351 Size: 378 6664 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 7042 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2413 2759 3338 2188 Size: 225 346 579 5892 Fragments arranged by size: 5892 579 346 225 FspI TGC'GCA Cuts at: 5548 5548 Size: 7042 HaeII r_GCGC'y Cuts at: 1678 2350 3274 3529 6189 1678 Size: 672 924 255 2660 2531 Fragments arranged by size: 2660 2531 924 672 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946 Size: 32 135 32 505 32 623 32 182 Cuts at: 2946 2978 5683 5715 5757 5789 1373 Size: 32 2705 32 42 32 2626 Fragments arranged by size: 2705 2626 623 505 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2529 4386 4632 669 Size: 988 281 115 476 1857 246 3079 Fragments arranged by size: 3079 1857 988 476 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 7042 HpaI GTT'AAC Cuts at: 2053 4632 2053 Size: 2579 4463 KpnI G_GTAC'C Cuts at: 2709 4367 2709 Size: 1658 5384 MfeI C'AATT_G Cuts at: 2988 4641 2988 Size: 1653 5389 MluI A'CGCG_T Cuts at: 6918 6918 Size: 7042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3889 6156 6340 1305 Size: 2584 2267 184 2007 Fragments arranged by size: 2584 2267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5220 Fragments arranged by size: 5220 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 4271 5000 5359 5518 518 Size: 648 1141 1964 729 359 159 2042 Fragments arranged by size: 2042 1964 1141 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 3398 4458 5135 6283 6528 1606 Size: 1156 636 1060 677 1148 245 2120 Fragments arranged by size: 2120 1156 1148 1060 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 2641 513 Size: 552 1576 4914 Fragments arranged by size: 4914 1576 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7042 NotI GC'GGCC_GC Cuts at: 4489 4489 Size: 7042 NsiI A_TGCA'T Cuts at: 3241 3422 3241 Size: 181 6861 NspI r_CATG'y Cuts at: 1132 1229 4400 5945 1132 Size: 97 3171 1545 2229 Fragments arranged by size: 3171 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7042 PciI A'CATG_T Cuts at: 5941 5941 Size: 7042 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7042 PmeI GTTT'AAAC Cuts at: 4346 4346 Size: 7042 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5111 5143 6624 6656 5111 Size: 32 1481 32 5497 Fragments arranged by size: 5497 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 4103 3094 Size: 1009 6033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7042 PsiI TTA'TAA Cuts at: 4612 4612 Size: 7042 PstI C_TGCA'G Cuts at: 830 3294 3562 4394 830 Size: 2464 268 832 3478 Fragments arranged by size: 3478 2464 832 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7042 PvuII CAG'CTG Cuts at: 2762 3398 2762 Size: 636 6406 SacI G_AGCT'C Cuts at: 3576 3576 Size: 7042 SalI G'TCGA_C Cuts at: 1936 4384 1936 Size: 2448 4594 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 7042 SbfI CC_TGCA'GG Cuts at: 4394 4394 Size: 7042 ScaI AGT'ACT Cuts at: 5290 7002 5290 Size: 1712 5330 SexAI A'CCwGG_T Cuts at: 3739 3739 Size: 7042 SfcI C'TryA_G Cuts at: 826 1045 3290 3558 4390 5525 6206 6397 826 Size: 219 2245 268 832 1135 681 191 1471 Fragments arranged by size: 2245 1471 1135 832 681 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7042 SmaI CCC'GGG Cuts at: 1872 4369 1872 Size: 2497 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5146 6047 6309 820 Size: 197 926 3203 901 262 1553 Fragments arranged by size: 3203 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4833 493 Size: 4340 2702 SpeI A'CTAG_T Cuts at: 152 3638 152 Size: 3486 3556 SphI G_CATG'C Cuts at: 1132 4400 1132 Size: 3268 3774 SspI AAT'ATT Cuts at: 5 52 4966 5 Size: 47 4914 2081 Fragments arranged by size: 4914 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7042 StyI C'CwwG_G Cuts at: 513 1065 2119 2532 2641 2791 4188 4481 513 Size: 552 1054 413 109 150 1397 293 3074 Fragments arranged by size: 3074 1397 1054 552 413 293 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5069 5086 5239 5424 5869 325 Size: 1255 3489 17 153 185 445 1498 Fragments arranged by size: 3489 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5288 7000 96 Size: 274 80 33 51 1443 3311 1712 138 Fragments arranged by size: 3311 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2871 2876 2903 3436 3468 4041 4073 7002 Size: 27 5 27 533 32 573 32 2929 Cuts at: 7002 7034 2844 Size: 32 2852 Fragments arranged by size: 2929 2852 573 533 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7042 XbaI T'CTAG_A Cuts at: 4310 4378 4310 Size: 68 6974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 7042 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7042 XmaI C'CCGG_G Cuts at: 1870 4367 1870 Size: 2497 4545 XmnI GAAnn'nnTTC Cuts at: 2171 5171 2171 Size: 3000 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4850 276 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI