(Linear) (Six-base) MAPSORT of: KIEE5758.seq Check: 4008 from: 1 to: 2337 KIEE5758.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. Acc65I G'GTAC_C Cuts at: 0 700 2337 Size: 700 1637 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 2176 2236 2337 Size: 153 2023 60 101 Fragments arranged by size: 2023 153 101 60 AfeI AGC'GCT Cuts at: 0 343 1522 2337 Size: 343 1179 815 Fragments arranged by size: 1179 815 343 AleI CACnn'nnGTG Cuts at: 0 2266 2337 Size: 2266 71 AlwNI CAG_nnn'CTG Cuts at: 0 2101 2337 Size: 2101 236 ApoI r'AATT_y Cuts at: 0 1407 1649 2194 2337 Size: 1407 242 545 143 Fragments arranged by size: 1407 545 242 143 AseI AT'TA_AT Cuts at: 0 509 1279 1939 2337 Size: 509 770 660 398 Fragments arranged by size: 770 660 509 398 BamHI G'GATC_C Cuts at: 0 388 2337 Size: 388 1949 BanI G'GyrC_C Cuts at: 0 151 700 1218 2337 Size: 151 549 518 1119 Fragments arranged by size: 1119 549 518 151 BanII G_rGCy'C Cuts at: 0 1571 2337 Size: 1571 766 BbsI GAAGACnn'nnnn_ Cuts at: 0 1368 2316 2337 Size: 1368 948 21 Fragments arranged by size: 1368 948 21 BbvCI CC'TCA_GC Cuts at: 0 1537 2337 Size: 1537 800 BclI T'GATC_A Cuts at: 0 1083 1911 2337 Size: 1083 828 426 Fragments arranged by size: 1083 828 426 BfrBI ATG'CAT Cuts at: 0 1234 1415 2337 Size: 1234 181 922 Fragments arranged by size: 1234 922 181 BglII A'GATC_T Cuts at: 0 80 2337 Size: 80 2257 Bme1580I G_kGCm'C Cuts at: 0 135 154 2337 Size: 135 19 2183 Fragments arranged by size: 2183 135 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1604 2150 2337 Size: 910 694 546 187 Fragments arranged by size: 910 694 546 187 Bpu10I CC'TnA_GC Cuts at: 0 1537 2337 Size: 1537 800 BsaHI Gr'CG_yC Cuts at: 0 353 2337 Size: 353 1984 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 941 971 1431 1461 2036 2066 Size: 866 30 45 30 460 30 575 30 Cuts at: 2066 2337 Size: 271 Fragments arranged by size: 866 575 460 271 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 965 1870 2337 Size: 385 580 905 467 Fragments arranged by size: 905 580 467 385 BseYI C'CCAG_C Cuts at: 0 276 2337 Size: 276 2061 BsiHKAI G_wGCw'C Cuts at: 0 627 1571 2337 Size: 627 944 766 Fragments arranged by size: 944 766 627 BsmI GAATG_Cn' Cuts at: 0 492 2337 Size: 492 1845 BsmBI CGTCTCn'nnnn_ Cuts at: 0 788 958 2337 Size: 788 170 1379 Fragments arranged by size: 1379 788 170 Bsp1286I G_dGCh'C Cuts at: 0 135 154 627 1571 2337 Size: 135 19 473 944 766 Fragments arranged by size: 944 766 473 135 19 BspHI T'CATG_A Cuts at: 0 1841 2337 Size: 1841 496 BssSI C'ACGA_G Cuts at: 0 848 2075 2337 Size: 848 1227 262 Fragments arranged by size: 1227 848 262 BstEII G'GTnAC_C Cuts at: 0 2178 2187 2337 Size: 2178 9 150 Fragments arranged by size: 2178 150 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 2337 Size: 304 2033 BstYI r'GATC_y Cuts at: 0 80 388 1155 2302 2337 Size: 80 308 767 1147 35 Fragments arranged by size: 1147 767 308 80 35 BtgI C'CryG_G Cuts at: 0 636 2337 Size: 636 1701 DraI TTT'AAA Cuts at: 0 1754 2337 Size: 1754 583 DraIII CAC_nnn'GTG Cuts at: 0 853 2337 Size: 853 1484 EarI CTCTTCn'nnn_ Cuts at: 0 786 1679 2168 2337 Size: 786 893 489 169 Fragments arranged by size: 893 786 489 169 EcoICRI GAG'CTC Cuts at: 0 1569 2337 Size: 1569 768 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 910 1604 2150 2176 2236 2337 Size: 153 757 694 546 26 60 101 Fragments arranged by size: 757 694 546 153 101 60 26 EcoO109I rG'GnC_Cy Cuts at: 0 783 1089 2098 2337 Size: 783 306 1009 239 Fragments arranged by size: 1009 783 306 239 EcoRV GAT'ATC Cuts at: 0 444 2337 Size: 444 1893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 408 754 1333 2337 Size: 183 225 346 579 1004 Fragments arranged by size: 1004 579 346 225 183 HaeII r_GCGC'y Cuts at: 0 345 1269 1524 2337 Size: 345 924 255 813 Fragments arranged by size: 924 813 345 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 72 104 727 759 941 973 2337 Size: 72 32 623 32 182 32 1364 Fragments arranged by size: 1364 623 182 72 32 32 32 HincII GTy'rAC Cuts at: 0 48 524 2337 Size: 48 476 1813 Fragments arranged by size: 1813 476 48 HpaI GTT'AAC Cuts at: 0 48 2337 Size: 48 2289 KpnI G_GTAC'C Cuts at: 0 704 2337 Size: 704 1633 MfeI C'AATT_G Cuts at: 0 983 2337 Size: 983 1354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1884 2337 Size: 1884 453 MslI CAynn'nnrTG Cuts at: 0 302 2266 2337 Size: 302 1964 71 Fragments arranged by size: 1964 302 71 MspA1I CmG'CkG Cuts at: 0 757 1393 2337 Size: 757 636 944 Fragments arranged by size: 944 757 636 NcoI C'CATG_G Cuts at: 0 636 2337 Size: 636 1701 NsiI A_TGCA'T Cuts at: 0 1236 1417 2337 Size: 1236 181 920 Fragments arranged by size: 1236 920 181 PpuMI rG'GwC_Cy Cuts at: 0 1089 2098 2337 Size: 1089 1009 239 Fragments arranged by size: 1089 1009 239 PstI C_TGCA'G Cuts at: 0 1289 1557 2337 Size: 1289 268 780 Fragments arranged by size: 1289 780 268 PvuII CAG'CTG Cuts at: 0 757 1393 2337 Size: 757 636 944 Fragments arranged by size: 944 757 636 SacI G_AGCT'C Cuts at: 0 1571 2337 Size: 1571 766 SapI GCTCTTCn'nnn_ Cuts at: 0 1679 2337 Size: 1679 658 SexAI A'CCwGG_T Cuts at: 0 1734 2337 Size: 1734 603 SfcI C'TryA_G Cuts at: 0 1285 1553 2337 Size: 1285 268 784 Fragments arranged by size: 1285 784 268 SpeI A'CTAG_T Cuts at: 0 1633 2337 Size: 1633 704 StyI C'CwwG_G Cuts at: 0 114 527 636 786 2183 2337 Size: 114 413 109 150 1397 154 Fragments arranged by size: 1397 413 154 150 114 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 866 871 898 1431 1463 2036 2068 Size: 839 27 5 27 533 32 573 32 Cuts at: 2068 2337 Size: 269 Fragments arranged by size: 839 573 533 269 32 32 27 27 5 XbaI T'CTAG_A Cuts at: 0 2305 2337 Size: 2305 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 598 2337 Size: 598 1739 XmnI GAAnn'nnTTC Cuts at: 0 166 2337 Size: 166 2171 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI MspA1I NcoI NsiI PpuMI PstI PvuII SacI SapI SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoRI FseI FspI FspAI HindIII KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI