(Circular) (Six-base) MAPSORT of: pFN21AE2777.seq Check: 1101 from: 1 to: 7213 pFN21AE2777 7213 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5023 278 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4556 1937 Size: 2619 4594 Acc65I G'GTAC_C Cuts at: 2705 4534 2705 Size: 1829 5384 AclI AA'CG_TT Cuts at: 1563 5340 5713 1563 Size: 3777 373 3063 Fragments arranged by size: 3777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 4055 4110 4188 4352 4412 5276 6660 Size: 526 1897 55 78 164 60 864 1384 Cuts at: 6660 6928 1632 Size: 268 1917 Fragments arranged by size: 1917 1897 1384 864 526 268 164 78 60 55 AfeI AGC'GCT Cuts at: 2348 3527 2348 Size: 1179 6034 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7016 AflIII A'CryG_T Cuts at: 1203 6112 7089 1203 Size: 4909 977 1327 Fragments arranged by size: 4909 1327 977 AgeI A'CCGG_T Cuts at: 6044 6044 Size: 7213 AhdI GACnn_n'nnGTC Cuts at: 5942 5942 Size: 7213 AleI CACnn'nnGTG Cuts at: 1166 4442 1166 Size: 3276 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7213 AlwNI CAG_nnn'CTG Cuts at: 4277 4956 6528 4277 Size: 679 1572 4962 Fragments arranged by size: 4962 1572 679 ApaLI G'TGCA_C Cuts at: 5270 6426 5270 Size: 1156 6057 ApoI r'AATT_y Cuts at: 1493 3412 3654 4133 4370 4522 4748 4900 7009 Size: 1919 242 479 237 152 226 152 2109 Cuts at: 7009 1493 Size: 1697 Fragments arranged by size: 2109 1919 1697 479 242 237 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 2514 3284 3944 5767 160 Size: 872 1482 770 660 1823 1606 Fragments arranged by size: 1823 1606 1482 872 770 660 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 AvaI C'yCGr_G Cuts at: 1870 1943 4538 1870 Size: 73 2595 4545 Fragments arranged by size: 4545 2595 73 BamHI G'GATC_C Cuts at: 2393 4543 4910 2393 Size: 2150 367 4696 Fragments arranged by size: 4696 2150 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2705 3223 4534 5989 Size: 325 217 627 369 549 518 1311 1455 Cuts at: 5989 618 Size: 1842 Fragments arranged by size: 1842 1455 1311 627 549 518 369 325 217 BanII G_rGCy'C Cuts at: 1391 3576 4532 1391 Size: 2185 956 4072 Fragments arranged by size: 4072 2185 956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 4061 4492 928 Size: 971 1474 688 431 3649 Fragments arranged by size: 3649 1474 971 688 431 BbvCI CC'TCA_GC Cuts at: 3542 3542 Size: 7213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5404 5438 1903 Size: 34 3467 34 3678 Fragments arranged by size: 3678 3467 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5105 6315 5105 Size: 1210 6003 BclI T'GATC_A Cuts at: 1548 3088 3916 1548 Size: 1540 828 4845 Fragments arranged by size: 4845 1540 828 BfrBI ATG'CAT Cuts at: 3239 3420 3239 Size: 181 7032 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5824 136 Size: 107 122 71 631 605 4152 1525 Fragments arranged by size: 4152 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 7208 2085 Size: 5123 2090 BlpI GC'TnA_GC Cuts at: 4630 4630 Size: 7213 Bme1580I G_kGCm'C Cuts at: 1790 2140 2159 5274 6430 1790 Size: 350 19 3115 1156 2573 Fragments arranged by size: 3115 2573 1156 350 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4944 5902 442 Size: 416 53 83 399 24 3527 958 1753 Fragments arranged by size: 3527 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 3609 4326 5873 1239 Size: 1676 694 717 1547 2579 Fragments arranged by size: 2579 1676 1547 717 694 Bpu10I CC'TnA_GC Cuts at: 3542 3542 Size: 7213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5338 6203 6501 5338 Size: 865 298 6050 Fragments arranged by size: 6050 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5876 882 Size: 617 4377 2219 Fragments arranged by size: 4377 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5004 493 Size: 711 3800 2702 Fragments arranged by size: 3800 2702 711 BsaBI GATnn'nnATC Cuts at: 7207 7207 Size: 7213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 5020 5402 6037 Size: 53 83 186 936 825 2662 382 635 Cuts at: 6037 275 Size: 1451 Fragments arranged by size: 2662 1451 936 825 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5646 6044 6318 6465 6876 5646 Size: 398 274 147 411 5983 Fragments arranged by size: 5983 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436 Size: 30 165 30 1468 30 45 30 460 Cuts at: 3436 3466 4212 4242 1178 Size: 30 746 30 4149 Fragments arranged by size: 4149 1468 746 460 165 45 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2970 3875 1186 Size: 1204 580 905 4524 Fragments arranged by size: 4524 1204 905 580 BseYI C'CCAG_C Cuts at: 2281 6416 6912 2281 Size: 4135 496 2582 Fragments arranged by size: 4135 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6640 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4663 5424 6452 1540 Size: 275 186 2662 761 1028 2301 Fragments arranged by size: 2662 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3576 5274 5359 6430 2632 Size: 944 1698 85 1071 3415 Fragments arranged by size: 3415 1698 1071 944 85 BsmI GAATG_Cn' Cuts at: 2497 4724 4817 2497 Size: 2227 93 4893 Fragments arranged by size: 4893 2227 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2963 2793 Size: 170 7043 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2159 2632 3576 4532 5274 5359 Size: 399 350 19 473 944 956 742 85 Cuts at: 5359 6430 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 956 944 742 473 399 350 85 19 BspEI T'CCGG_A Cuts at: 6876 6876 Size: 7213 BspHI T'CATG_A Cuts at: 3846 5102 6832 3846 Size: 1256 1730 4227 Fragments arranged by size: 4227 1730 1256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4568 844 Size: 3724 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5100 1394 Size: 429 3277 3507 Fragments arranged by size: 3507 3277 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5708 5882 65 Size: 1183 4460 174 1396 Fragments arranged by size: 4460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5857 6044 1700 Size: 110 4047 187 2869 Fragments arranged by size: 4047 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7213 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7213 BssSI C'ACGA_G Cuts at: 2853 4251 5273 6285 2853 Size: 1398 1022 1012 3781 Fragments arranged by size: 3781 1398 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7213 BstBI TT'CG_AA Cuts at: 6017 6017 Size: 7213 BstEII G'GTnAC_C Cuts at: 1184 4354 4363 1184 Size: 3170 9 4034 Fragments arranged by size: 4034 3170 9 BstXI CCAn_nnnn'nTGG Cuts at: 2309 4036 2309 Size: 1727 5486 BstYI r'GATC_y Cuts at: 1972 2085 2393 3160 4478 4543 4910 5295 5312 Size: 113 308 767 1318 65 367 385 17 Cuts at: 5312 6764 6850 6862 7208 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1318 767 385 367 346 308 113 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 4177 513 Size: 552 1576 1536 3549 Fragments arranged by size: 3549 1576 1536 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6474 Fragments arranged by size: 6474 683 56 BtsI GCAGTG_nn' Cuts at: 4729 5523 5543 4729 Size: 794 20 6399 Fragments arranged by size: 6399 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7178 DraI TTT'AAA Cuts at: 3759 4517 3759 Size: 758 6455 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 7213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6220 809 Size: 507 4904 1802 Fragments arranged by size: 4904 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4660 5549 7178 Size: 54 1425 50 275 18 2830 889 1629 Cuts at: 7178 8 Size: 43 Fragments arranged by size: 2830 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4660 1537 Size: 275 2848 4090 Fragments arranged by size: 4090 2848 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 3684 4344 5143 1353 Size: 1438 893 660 799 3423 Fragments arranged by size: 3423 1438 893 799 660 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5800 6174 6320 387 Size: 1064 138 4211 374 146 1280 Fragments arranged by size: 4211 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 3574 Size: 7213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2915 3609 4055 4110 4188 4326 Size: 393 526 757 694 446 55 78 138 Cuts at: 4326 4352 4412 5276 5873 6660 6928 1239 Size: 26 60 864 597 787 268 1524 Fragments arranged by size: 1524 864 787 757 694 597 526 446 393 268 138 78 60 55 26 EcoO109I rG'GnC_Cy Cuts at: 2788 3094 4094 4274 2788 Size: 306 1000 180 5727 Fragments arranged by size: 5727 1000 306 180 EcoRI G'AATT_C Cuts at: 4133 4522 4900 4133 Size: 389 378 6446 Fragments arranged by size: 6446 389 378 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 7213 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2413 2759 3338 4085 2188 Size: 225 346 579 747 5316 Fragments arranged by size: 5316 747 579 346 225 FspI TGC'GCA Cuts at: 5719 5719 Size: 7213 HaeII r_GCGC'y Cuts at: 1678 2350 3274 3529 6360 1678 Size: 672 924 255 2831 2531 Fragments arranged by size: 2831 2531 924 672 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946 Size: 32 135 32 505 32 623 32 182 Cuts at: 2946 2978 5854 5886 5928 5960 1373 Size: 32 2876 32 42 32 2626 Fragments arranged by size: 2876 2626 623 505 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2529 4557 4803 669 Size: 988 281 115 476 2028 246 3079 Fragments arranged by size: 3079 2028 988 476 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 7213 HpaI GTT'AAC Cuts at: 2053 4803 2053 Size: 2750 4463 KpnI G_GTAC'C Cuts at: 2709 4538 2709 Size: 1829 5384 MfeI C'AATT_G Cuts at: 2988 4812 2988 Size: 1824 5389 MluI A'CGCG_T Cuts at: 7089 7089 Size: 7213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3889 6327 6511 1305 Size: 2584 2438 184 2007 Fragments arranged by size: 2584 2438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5391 Fragments arranged by size: 5391 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 4442 5171 5530 5689 518 Size: 648 1141 2135 729 359 159 2042 Fragments arranged by size: 2135 2042 1141 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 3398 4629 5306 6454 6699 1606 Size: 1156 636 1231 677 1148 245 2120 Fragments arranged by size: 2120 1231 1156 1148 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 2641 4177 513 Size: 552 1576 1536 3549 Fragments arranged by size: 3549 1576 1536 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7213 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7213 NotI GC'GGCC_GC Cuts at: 4660 4660 Size: 7213 NsiI A_TGCA'T Cuts at: 3241 3422 3241 Size: 181 7032 NspI r_CATG'y Cuts at: 1132 1229 4571 6116 1132 Size: 97 3342 1545 2229 Fragments arranged by size: 3342 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7213 PciI A'CATG_T Cuts at: 6112 6112 Size: 7213 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7213 PmeI GTTT'AAAC Cuts at: 4517 4517 Size: 7213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5282 5314 6795 6827 5282 Size: 32 1481 32 5668 Fragments arranged by size: 5668 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 4094 4274 3094 Size: 1000 180 6033 Fragments arranged by size: 6033 1000 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7213 PsiI TTA'TAA Cuts at: 4025 4783 4025 Size: 758 6455 PstI C_TGCA'G Cuts at: 830 3294 3562 4565 830 Size: 2464 268 1003 3478 Fragments arranged by size: 3478 2464 1003 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7213 PvuII CAG'CTG Cuts at: 2762 3398 2762 Size: 636 6577 SacI G_AGCT'C Cuts at: 3576 3576 Size: 7213 SalI G'TCGA_C Cuts at: 1936 4555 1936 Size: 2619 4594 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 7213 SbfI CC_TGCA'GG Cuts at: 4565 4565 Size: 7213 ScaI AGT'ACT Cuts at: 5461 7173 5461 Size: 1712 5501 SexAI A'CCwGG_T Cuts at: 3739 3739 Size: 7213 SfcI C'TryA_G Cuts at: 826 1045 3290 3558 4561 5696 6377 6568 826 Size: 219 2245 268 1003 1135 681 191 1471 Fragments arranged by size: 2245 1471 1135 1003 681 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7213 SmaI CCC'GGG Cuts at: 1872 4540 1872 Size: 2668 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5317 6218 6480 820 Size: 197 926 3374 901 262 1553 Fragments arranged by size: 3374 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5004 493 Size: 4511 2702 SpeI A'CTAG_T Cuts at: 152 3638 152 Size: 3486 3727 SphI G_CATG'C Cuts at: 1132 4571 1132 Size: 3439 3774 SspI AAT'ATT Cuts at: 5 52 5137 5 Size: 47 5085 2081 Fragments arranged by size: 5085 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7213 StyI C'CwwG_G Cuts at: 513 1065 2119 2532 2641 2791 4177 4359 4652 Size: 552 1054 413 109 150 1386 182 293 Cuts at: 4652 513 Size: 3074 Fragments arranged by size: 3074 1386 1054 552 413 293 182 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5240 5257 5410 5595 6040 325 Size: 1255 3660 17 153 185 445 1498 Fragments arranged by size: 3660 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5459 7171 96 Size: 274 80 33 51 1443 3482 1712 138 Fragments arranged by size: 3482 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2871 2876 2903 3436 3468 4212 4244 7173 Size: 27 5 27 533 32 744 32 2929 Cuts at: 7173 7205 2844 Size: 32 2852 Fragments arranged by size: 2929 2852 744 533 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7213 XbaI T'CTAG_A Cuts at: 4481 4549 4481 Size: 68 7145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 7213 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7213 XmaI C'CCGG_G Cuts at: 1870 4538 1870 Size: 2668 4545 XmnI GAAnn'nnTTC Cuts at: 2171 5342 2171 Size: 3171 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5021 276 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI