(Linear) (Six-base) MAPSORT of: KIEE2777.seq Check: 1594 from: 1 to: 2508 KIEE2777.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. Acc65I G'GTAC_C Cuts at: 0 700 2508 Size: 700 1808 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 2050 2105 2183 2347 2407 2508 Size: 153 1897 55 78 164 60 101 Fragments arranged by size: 1897 164 153 101 78 60 55 AfeI AGC'GCT Cuts at: 0 343 1522 2508 Size: 343 1179 986 Fragments arranged by size: 1179 986 343 AleI CACnn'nnGTG Cuts at: 0 2437 2508 Size: 2437 71 AlwNI CAG_nnn'CTG Cuts at: 0 2272 2508 Size: 2272 236 ApoI r'AATT_y Cuts at: 0 1407 1649 2128 2365 2508 Size: 1407 242 479 237 143 Fragments arranged by size: 1407 479 242 237 143 AseI AT'TA_AT Cuts at: 0 509 1279 1939 2508 Size: 509 770 660 569 Fragments arranged by size: 770 660 569 509 BamHI G'GATC_C Cuts at: 0 388 2508 Size: 388 2120 BanI G'GyrC_C Cuts at: 0 151 700 1218 2508 Size: 151 549 518 1290 Fragments arranged by size: 1290 549 518 151 BanII G_rGCy'C Cuts at: 0 1571 2508 Size: 1571 937 BbsI GAAGACnn'nnnn_ Cuts at: 0 1368 2056 2487 2508 Size: 1368 688 431 21 Fragments arranged by size: 1368 688 431 21 BbvCI CC'TCA_GC Cuts at: 0 1537 2508 Size: 1537 971 BclI T'GATC_A Cuts at: 0 1083 1911 2508 Size: 1083 828 597 Fragments arranged by size: 1083 828 597 BfrBI ATG'CAT Cuts at: 0 1234 1415 2508 Size: 1234 181 1093 Fragments arranged by size: 1234 1093 181 BglII A'GATC_T Cuts at: 0 80 2508 Size: 80 2428 Bme1580I G_kGCm'C Cuts at: 0 135 154 2508 Size: 135 19 2354 Fragments arranged by size: 2354 135 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1604 2321 2508 Size: 910 694 717 187 Fragments arranged by size: 910 717 694 187 Bpu10I CC'TnA_GC Cuts at: 0 1537 2508 Size: 1537 971 BsaHI Gr'CG_yC Cuts at: 0 353 2508 Size: 353 2155 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 941 971 1431 1461 2207 2237 Size: 866 30 45 30 460 30 746 30 Cuts at: 2237 2508 Size: 271 Fragments arranged by size: 866 746 460 271 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 965 1870 2508 Size: 385 580 905 638 Fragments arranged by size: 905 638 580 385 BseYI C'CCAG_C Cuts at: 0 276 2508 Size: 276 2232 BsiHKAI G_wGCw'C Cuts at: 0 627 1571 2508 Size: 627 944 937 Fragments arranged by size: 944 937 627 BsmI GAATG_Cn' Cuts at: 0 492 2508 Size: 492 2016 BsmBI CGTCTCn'nnnn_ Cuts at: 0 788 958 2508 Size: 788 170 1550 Fragments arranged by size: 1550 788 170 Bsp1286I G_dGCh'C Cuts at: 0 135 154 627 1571 2508 Size: 135 19 473 944 937 Fragments arranged by size: 944 937 473 135 19 BspHI T'CATG_A Cuts at: 0 1841 2508 Size: 1841 667 BssSI C'ACGA_G Cuts at: 0 848 2246 2508 Size: 848 1398 262 Fragments arranged by size: 1398 848 262 BstEII G'GTnAC_C Cuts at: 0 2349 2358 2508 Size: 2349 9 150 Fragments arranged by size: 2349 150 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 2031 2508 Size: 304 1727 477 Fragments arranged by size: 1727 477 304 BstYI r'GATC_y Cuts at: 0 80 388 1155 2473 2508 Size: 80 308 767 1318 35 Fragments arranged by size: 1318 767 308 80 35 BtgI C'CryG_G Cuts at: 0 636 2172 2508 Size: 636 1536 336 Fragments arranged by size: 1536 636 336 DraI TTT'AAA Cuts at: 0 1754 2508 Size: 1754 754 DraIII CAC_nnn'GTG Cuts at: 0 853 2508 Size: 853 1655 EarI CTCTTCn'nnn_ Cuts at: 0 786 1679 2339 2508 Size: 786 893 660 169 Fragments arranged by size: 893 786 660 169 EcoICRI GAG'CTC Cuts at: 0 1569 2508 Size: 1569 939 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 910 1604 2050 2105 2183 2321 2347 Size: 153 757 694 446 55 78 138 26 Cuts at: 2347 2407 2508 Size: 60 101 Fragments arranged by size: 757 694 446 153 138 101 78 60 55 26 EcoO109I rG'GnC_Cy Cuts at: 0 783 1089 2089 2269 2508 Size: 783 306 1000 180 239 Fragments arranged by size: 1000 783 306 239 180 EcoRI G'AATT_C Cuts at: 0 2128 2508 Size: 2128 380 EcoRV GAT'ATC Cuts at: 0 444 2508 Size: 444 2064 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 408 754 1333 2080 2508 Size: 183 225 346 579 747 428 Fragments arranged by size: 747 579 428 346 225 183 HaeII r_GCGC'y Cuts at: 0 345 1269 1524 2508 Size: 345 924 255 984 Fragments arranged by size: 984 924 345 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 72 104 727 759 941 973 2508 Size: 72 32 623 32 182 32 1535 Fragments arranged by size: 1535 623 182 72 32 32 32 HincII GTy'rAC Cuts at: 0 48 524 2508 Size: 48 476 1984 Fragments arranged by size: 1984 476 48 HpaI GTT'AAC Cuts at: 0 48 2508 Size: 48 2460 KpnI G_GTAC'C Cuts at: 0 704 2508 Size: 704 1804 MfeI C'AATT_G Cuts at: 0 983 2508 Size: 983 1525 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1884 2508 Size: 1884 624 MslI CAynn'nnrTG Cuts at: 0 302 2437 2508 Size: 302 2135 71 Fragments arranged by size: 2135 302 71 MspA1I CmG'CkG Cuts at: 0 757 1393 2508 Size: 757 636 1115 Fragments arranged by size: 1115 757 636 NcoI C'CATG_G Cuts at: 0 636 2172 2508 Size: 636 1536 336 Fragments arranged by size: 1536 636 336 NsiI A_TGCA'T Cuts at: 0 1236 1417 2508 Size: 1236 181 1091 Fragments arranged by size: 1236 1091 181 PpuMI rG'GwC_Cy Cuts at: 0 1089 2089 2269 2508 Size: 1089 1000 180 239 Fragments arranged by size: 1089 1000 239 180 PsiI TTA'TAA Cuts at: 0 2020 2508 Size: 2020 488 PstI C_TGCA'G Cuts at: 0 1289 1557 2508 Size: 1289 268 951 Fragments arranged by size: 1289 951 268 PvuII CAG'CTG Cuts at: 0 757 1393 2508 Size: 757 636 1115 Fragments arranged by size: 1115 757 636 SacI G_AGCT'C Cuts at: 0 1571 2508 Size: 1571 937 SapI GCTCTTCn'nnn_ Cuts at: 0 1679 2508 Size: 1679 829 SexAI A'CCwGG_T Cuts at: 0 1734 2508 Size: 1734 774 SfcI C'TryA_G Cuts at: 0 1285 1553 2508 Size: 1285 268 955 Fragments arranged by size: 1285 955 268 SpeI A'CTAG_T Cuts at: 0 1633 2508 Size: 1633 875 StyI C'CwwG_G Cuts at: 0 114 527 636 786 2172 2354 2508 Size: 114 413 109 150 1386 182 154 Fragments arranged by size: 1386 413 182 154 150 114 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 866 871 898 1431 1463 2207 2239 Size: 839 27 5 27 533 32 744 32 Cuts at: 2239 2508 Size: 269 Fragments arranged by size: 839 744 533 269 32 32 27 27 5 XbaI T'CTAG_A Cuts at: 0 2476 2508 Size: 2476 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 598 2508 Size: 598 1910 XmnI GAAnn'nnTTC Cuts at: 0 166 2508 Size: 166 2342 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI MspA1I NcoI NsiI PpuMI PsiI PstI PvuII SacI SapI SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HindIII KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI