(Circular) (Six-base) MAPSORT of: pFN21AE0900.seq Check: 2045 from: 1 to: 14959 pFN21AE0900 14959 bp With 182 enzymes: SgfI * December 21, 2016 11:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7537 7537 Size: 14959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 12769 278 Size: 53 83 186 936 11233 2468 Fragments arranged by size: 11233 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2341 5532 6868 9626 10370 11830 12302 1937 Size: 404 3191 1336 2758 744 1460 472 4594 Fragments arranged by size: 4594 3191 2758 1460 1336 744 472 404 Acc65I G'GTAC_C Cuts at: 12280 12280 Size: 14959 AclI AA'CG_TT Cuts at: 1563 4494 13086 13459 1563 Size: 2931 8592 373 3063 Fragments arranged by size: 8592 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2744 2934 3093 3312 3624 3981 4662 Size: 465 647 190 159 219 312 357 681 Cuts at: 4662 5014 5718 7851 8415 9440 9717 11158 11586 Size: 352 704 2133 564 1025 277 1441 428 Cuts at: 11586 13022 14406 14674 1632 Size: 1436 1384 268 1917 Fragments arranged by size: 2133 1917 1441 1436 1384 1025 704 681 647 564 465 428 357 352 312 277 268 219 190 159 AflII C'TTAA_G Cuts at: 820 1017 6850 820 Size: 197 5833 8929 Fragments arranged by size: 8929 5833 197 AflIII A'CryG_T Cuts at: 1203 2452 4028 8768 9508 13858 14835 1203 Size: 1249 1576 4740 740 4350 977 1327 Fragments arranged by size: 4740 4350 1576 1327 1249 977 740 AgeI A'CCGG_T Cuts at: 11119 13790 11119 Size: 2671 12288 AhdI GACnn_n'nnGTC Cuts at: 4905 10245 13688 4905 Size: 5340 3443 6176 Fragments arranged by size: 6176 5340 3443 AleI CACnn'nnGTG Cuts at: 1166 10907 1166 Size: 9741 5218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 10707 10722 1184 Size: 9523 15 5421 Fragments arranged by size: 9523 5421 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 9579 9611 2313 Size: 32 7234 32 7661 Fragments arranged by size: 7661 7234 32 32 AlwNI CAG_nnn'CTG Cuts at: 4121 4640 9404 12702 14274 4121 Size: 519 4764 3298 1572 4806 Fragments arranged by size: 4806 4764 3298 1572 519 ApaLI G'TGCA_C Cuts at: 10668 13016 14172 10668 Size: 2348 1156 11455 Fragments arranged by size: 11455 2348 1156 ApoI r'AATT_y Cuts at: 1493 2043 2303 2410 2818 2997 3334 3670 3712 Size: 550 260 107 408 179 337 336 42 Cuts at: 3712 5381 5992 6008 6346 6391 6790 7000 7653 Size: 1669 611 16 338 45 399 210 653 Cuts at: 7653 7788 8155 8249 8390 8800 8879 9046 9068 Size: 135 367 94 141 410 79 167 22 Cuts at: 9068 9800 9855 9926 10608 10619 11025 11133 11180 Size: 732 55 71 682 11 406 108 47 Cuts at: 11180 11956 11984 11994 12268 12494 12646 14755 1493 Size: 776 28 10 274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1669 776 732 682 653 611 550 410 408 406 399 367 338 337 336 274 260 226 210 179 167 152 141 135 108 107 94 79 71 55 47 45 42 28 22 16 11 10 AseI AT'TA_AT Cuts at: 160 1032 5199 9351 13513 160 Size: 872 4167 4152 4162 1606 Fragments arranged by size: 4167 4162 4152 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 14959 AvaI C'yCGr_G Cuts at: 1870 1943 12284 1870 Size: 73 10341 4545 Fragments arranged by size: 10341 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8985 9018 8985 Size: 33 14926 BamHI G'GATC_C Cuts at: 2015 2569 9835 12289 12656 2015 Size: 554 7266 2454 367 4318 Fragments arranged by size: 7266 4318 2454 554 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6835 11918 12280 13735 618 Size: 325 217 627 5048 5083 362 1455 1842 Fragments arranged by size: 5083 5048 1842 1455 627 362 325 217 BanII G_rGCy'C Cuts at: 1391 3223 8832 9814 10286 11166 12278 1391 Size: 1832 5609 982 472 880 1112 4072 Fragments arranged by size: 5609 4072 1832 1112 982 880 472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 4669 8270 9446 10508 11649 928 Size: 971 446 2324 3601 1176 1062 1141 4238 Fragments arranged by size: 4238 3601 2324 1176 1141 1062 971 446 BbvCI CC'TCA_GC Cuts at: 2562 2562 Size: 14959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 12017 12051 13150 13184 1903 Size: 34 50 34 9996 34 1099 34 3678 Fragments arranged by size: 9996 3678 1099 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 12851 14061 12851 Size: 1210 13749 BclI T'GATC_A Cuts at: 1548 4288 6082 6355 12174 1548 Size: 2740 1794 273 5819 4333 Fragments arranged by size: 5819 4333 2740 1794 273 BfrBI ATG'CAT Cuts at: 7400 8971 11548 7400 Size: 1571 2577 10811 Fragments arranged by size: 10811 2577 1571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 13570 136 Size: 107 122 71 631 605 11898 1525 Fragments arranged by size: 11898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 14954 14954 Size: 14959 BlpI GC'TnA_GC Cuts at: 12376 12376 Size: 14959 Bme1580I G_kGCm'C Cuts at: 1790 10672 13020 14176 1790 Size: 8882 2348 1156 2573 Fragments arranged by size: 8882 2573 2348 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 14959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3853 4217 6485 Size: 416 53 83 399 24 2436 364 2268 Cuts at: 6485 10189 12690 13648 442 Size: 3704 2501 958 1753 Fragments arranged by size: 3704 2501 2436 2268 1753 958 416 399 364 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6588 1056 Size: 5532 9427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5205 5205 Size: 14959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3465 8094 8919 9810 11363 11445 13619 1239 Size: 2226 4629 825 891 1553 82 2174 2579 Fragments arranged by size: 4629 2579 2226 2174 1553 891 825 82 Bpu10I CC'TnA_GC Cuts at: 2562 6843 7009 9944 2562 Size: 4281 166 2935 7577 Fragments arranged by size: 7577 4281 2935 166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6174 10775 11845 13084 13949 14247 6174 Size: 4601 1070 1239 865 298 6886 Fragments arranged by size: 6886 4601 1239 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3195 6740 13622 882 Size: 617 1696 3545 6882 2219 Fragments arranged by size: 6882 3545 2219 1696 617 BsaAI yAC'GTr Cuts at: 493 1204 3347 8105 9630 10667 12750 493 Size: 711 2143 4758 1525 1037 2083 2702 Fragments arranged by size: 4758 2702 2143 2083 1525 1037 711 BsaBI GATnn'nnATC Cuts at: 3093 5453 5490 14953 3093 Size: 2360 37 9463 3099 Fragments arranged by size: 9463 3099 2360 37 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 10975 12766 13148 13783 Size: 53 83 186 936 9442 1791 382 635 Cuts at: 13783 275 Size: 1451 Fragments arranged by size: 9442 1791 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 11119 13392 13790 14064 14211 14622 11119 Size: 2273 398 274 147 411 11456 Fragments arranged by size: 11456 2273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 3137 3167 9579 Size: 30 165 30 1147 30 557 30 6412 Cuts at: 9579 9609 10431 10461 1178 Size: 30 822 30 5676 Fragments arranged by size: 6412 5676 1147 822 557 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4877 8838 11952 1186 Size: 3691 3961 3114 4193 Fragments arranged by size: 4193 3961 3691 3114 BseYI C'CCAG_C Cuts at: 10744 14162 14658 10744 Size: 3418 496 11045 Fragments arranged by size: 11045 3418 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5196 10155 10728 11868 1163 Size: 573 3460 4959 573 1140 4254 Fragments arranged by size: 4959 4254 3460 1140 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 12409 13170 14198 1540 Size: 275 186 10408 761 1028 2301 Fragments arranged by size: 10408 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2631 8352 10672 13020 13105 14176 2631 Size: 5721 2320 2348 85 1071 3414 Fragments arranged by size: 5721 3414 2348 2320 1071 85 BsmI GAATG_Cn' Cuts at: 3444 3736 3922 5483 5515 7750 9466 10738 12470 Size: 292 186 1561 32 2235 1716 1272 1732 Cuts at: 12470 12563 3444 Size: 93 5840 Fragments arranged by size: 5840 2235 1732 1716 1561 1272 292 186 93 32 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2631 3223 8352 8832 9814 10286 10672 Size: 399 841 592 5129 480 982 472 386 Cuts at: 10672 11166 12278 13020 13105 14176 1391 Size: 494 1112 742 85 1071 2174 Fragments arranged by size: 5129 2174 1112 1071 982 841 742 592 494 480 472 399 386 85 BspEI T'CCGG_A Cuts at: 14622 14622 Size: 14959 BspHI T'CATG_A Cuts at: 2671 2710 4291 4353 4732 5800 12848 14578 2671 Size: 39 1581 62 379 1068 7048 1730 3052 Fragments arranged by size: 7048 3052 1730 1581 1068 379 62 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2149 3725 7537 7667 8387 12314 844 Size: 1305 1576 3812 130 720 3927 3489 Fragments arranged by size: 3927 3812 3489 1576 1305 720 130 BsrBI CCG'CTC Cuts at: 1394 1823 12846 1394 Size: 429 11023 3507 Fragments arranged by size: 11023 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 9646 10537 11530 13454 13628 65 Size: 1183 8398 891 993 1924 174 1396 Fragments arranged by size: 8398 1924 1396 1183 993 891 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11119 13603 13790 1700 Size: 110 9309 2484 187 2869 Fragments arranged by size: 9309 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 2447 10298 11864 96 Size: 2351 7851 1566 3191 Fragments arranged by size: 7851 3191 2351 1566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 14959 BssSI C'ACGA_G Cuts at: 8325 13019 14031 8325 Size: 4694 1012 9253 Fragments arranged by size: 9253 4694 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 11554 794 Size: 10760 4199 BstBI TT'CG_AA Cuts at: 13763 13763 Size: 14959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 14959 BstYI r'GATC_y Cuts at: 1972 2015 2569 4057 9835 10201 10833 11188 12289 Size: 43 554 1488 5778 366 632 355 1101 Cuts at: 12289 12656 13041 13058 14510 14596 14608 14954 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 5778 1977 1488 1452 1101 632 554 385 367 366 355 346 86 43 17 12 BstZ17I GTA'TAC Cuts at: 9627 10371 9627 Size: 744 14215 Bsu36I CC'TnA_GG Cuts at: 9921 9921 Size: 14959 BtgI C'CryG_G Cuts at: 513 1065 4446 4782 10857 11156 513 Size: 552 3381 336 6075 299 4316 Fragments arranged by size: 6075 4316 3381 552 336 299 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 10407 487 Size: 683 56 9181 5039 Fragments arranged by size: 9181 5039 683 56 BtsI GCAGTG_nn' Cuts at: 6025 10731 11613 12475 13269 13289 6025 Size: 4706 882 862 794 20 7695 Fragments arranged by size: 7695 4706 882 862 794 20 ClaI AT'CG_AT Cuts at: 1554 5519 1554 Size: 3965 10994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 10548 10583 529 Size: 35 9984 35 4905 Fragments arranged by size: 9984 4905 35 35 DraI TTT'AAA Cuts at: 2191 2827 3592 4476 4917 8053 8395 9235 11980 Size: 636 765 884 441 3136 342 840 2745 Cuts at: 11980 12263 2191 Size: 283 4887 Fragments arranged by size: 4887 3136 2745 884 840 765 636 441 342 283 DraIII CAC_nnn'GTG Cuts at: 7847 9420 7847 Size: 1573 13386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2085 2349 4995 11755 13966 809 Size: 507 769 264 2646 6760 2211 1802 Fragments arranged by size: 6760 2646 2211 1802 769 507 264 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3692 6112 10152 Size: 54 1425 50 275 18 1862 2420 4040 Cuts at: 10152 11706 12406 13295 14924 8 Size: 1554 700 889 1629 43 Fragments arranged by size: 4040 2420 1862 1629 1554 1425 889 700 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 12406 1537 Size: 275 10594 4090 Fragments arranged by size: 10594 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3408 4191 5075 5414 7459 8815 10767 11803 Size: 2055 783 884 339 2045 1356 1952 1036 Cuts at: 11803 12889 1353 Size: 1086 3423 Fragments arranged by size: 3423 2055 2045 1952 1356 1086 1036 884 783 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 13546 13920 14066 387 Size: 1064 138 11957 374 146 1280 Fragments arranged by size: 11957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2744 2934 3093 3312 3465 3624 Size: 393 465 647 190 159 219 153 159 Cuts at: 3624 3981 4662 5014 5718 7851 8094 8415 8919 Size: 357 681 352 704 2133 243 321 504 Cuts at: 8919 9440 9717 9810 11158 11363 11445 11586 13022 Size: 521 277 93 1348 205 82 141 1436 Cuts at: 13022 13619 14406 14674 1239 Size: 597 787 268 1524 Fragments arranged by size: 2133 1524 1436 1348 787 704 681 647 597 521 504 465 393 357 352 321 277 268 243 219 205 190 159 159 153 141 93 82 EcoNI CCTnn'n_nnAGG Cuts at: 11333 11634 11333 Size: 301 14658 EcoRI G'AATT_C Cuts at: 2818 3334 3712 6790 11956 12268 12646 2818 Size: 516 378 3078 5166 312 378 5131 Fragments arranged by size: 5166 5131 3078 516 378 378 312 EcoRV GAT'ATC Cuts at: 7289 7289 Size: 14959 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3338 3905 5802 8050 8537 3338 Size: 567 1897 2248 487 9760 Fragments arranged by size: 9760 2248 1897 567 487 FspI TGC'GCA Cuts at: 13465 13465 Size: 14959 HaeII r_GCGC'y Cuts at: 1678 14106 1678 Size: 12428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3137 3169 3782 3814 3818 Size: 32 135 32 1565 32 613 32 4 Cuts at: 3818 3850 4115 4147 4250 4282 4325 4357 4891 Size: 32 265 32 103 32 43 32 534 Cuts at: 4891 4904 4923 4936 5818 5850 7631 7663 9025 Size: 13 19 13 882 32 1781 32 1362 Cuts at: 9025 9057 10231 10263 10490 10522 11644 11665 11676 Size: 32 1174 32 227 32 1122 21 11 Cuts at: 11676 11697 13600 13632 13674 13706 1373 Size: 21 1903 32 42 32 2626 Fragments arranged by size: 2626 1903 1781 1565 1362 1174 1122 882 613 534 265 227 135 103 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 21 19 13 13 11 4 HincII GTy'rAC Cuts at: 669 1657 1938 6231 7106 11004 12303 12549 669 Size: 988 281 4293 875 3898 1299 246 3079 Fragments arranged by size: 4293 3898 3079 1299 988 875 281 246 HindIII A'AGCT_T Cuts at: 748 7895 8700 9842 11540 11645 748 Size: 7147 805 1142 1698 105 4062 Fragments arranged by size: 7147 4062 1698 1142 805 105 HpaI GTT'AAC Cuts at: 6231 11004 12549 6231 Size: 4773 1545 8641 Fragments arranged by size: 8641 4773 1545 KpnI G_GTAC'C Cuts at: 12284 12284 Size: 14959 MfeI C'AATT_G Cuts at: 12558 12558 Size: 14959 MluI A'CGCG_T Cuts at: 9508 14835 9508 Size: 5327 9632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 10821 14073 14257 1305 Size: 9516 3252 184 2007 Fragments arranged by size: 9516 3252 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3694 6114 10154 11708 10 Size: 54 1425 343 1862 2420 4040 1554 3261 Fragments arranged by size: 4040 3261 2420 1862 1554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4296 6057 6927 7263 9090 10907 11562 Size: 648 3130 1761 870 336 1827 1817 655 Cuts at: 11562 12917 13276 13435 518 Size: 1355 359 159 2042 Fragments arranged by size: 3130 2042 1827 1817 1761 1355 870 655 648 359 336 159 MspA1I CmG'CkG Cuts at: 1606 2224 2272 6764 9404 9622 10049 12375 13052 Size: 618 48 4492 2640 218 427 2326 677 Cuts at: 13052 14200 14445 1606 Size: 1148 245 2120 Fragments arranged by size: 4492 2640 2326 2120 1148 677 618 427 245 218 48 NcoI C'CATG_G Cuts at: 513 1065 4446 4782 10857 513 Size: 552 3381 336 6075 4615 Fragments arranged by size: 6075 4615 3381 552 336 NdeI CA'TA_TG Cuts at: 387 3573 6095 9887 11355 387 Size: 3186 2522 3792 1468 3991 Fragments arranged by size: 3991 3792 3186 2522 1468 NheI G'CTAG_C Cuts at: 1052 6584 1052 Size: 5532 9427 NotI GC'GGCC_GC Cuts at: 12406 12406 Size: 14959 NsiI A_TGCA'T Cuts at: 7402 8973 11550 7402 Size: 1571 2577 10811 Fragments arranged by size: 10811 2577 1571 NspI r_CATG'y Cuts at: 1132 1229 2456 4032 4218 6201 6744 7240 8772 Size: 97 1227 1576 186 1983 543 496 1532 Cuts at: 8772 11561 12317 13862 1132 Size: 2789 756 1545 2229 Fragments arranged by size: 2789 2229 1983 1576 1545 1532 1227 756 543 496 186 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 14959 PciI A'CATG_T Cuts at: 2452 4028 8768 13858 2452 Size: 1576 4740 5090 3553 Fragments arranged by size: 5090 4740 3553 1576 PflMI CCAn_nnn'nTGG Cuts at: 1108 5522 10150 1108 Size: 4414 4628 5917 Fragments arranged by size: 5917 4628 4414 PmeI GTTT'AAAC Cuts at: 12263 12263 Size: 14959 PmlI CAC'GTG Cuts at: 10667 10667 Size: 14959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3997 4029 9579 9611 13028 13060 14541 14573 3997 Size: 32 5550 32 3417 32 1481 32 4383 Fragments arranged by size: 5550 4383 3417 1481 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 14959 PsiI TTA'TAA Cuts at: 2217 3804 4068 4488 9867 12529 2217 Size: 1587 264 420 5379 2662 4647 Fragments arranged by size: 5379 4647 2662 1587 420 264 PstI C_TGCA'G Cuts at: 830 2146 4143 4213 5764 7129 8384 12311 830 Size: 1316 1997 70 1551 1365 1255 3927 3478 Fragments arranged by size: 3927 3478 1997 1551 1365 1316 1255 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 14959 PvuII CAG'CTG Cuts at: 2224 6764 9404 9622 10049 2224 Size: 4540 2640 218 427 7134 Fragments arranged by size: 7134 4540 2640 427 218 SalI G'TCGA_C Cuts at: 1936 12301 1936 Size: 10365 4594 SapI GCTCTTCn'nnn_ Cuts at: 3408 5075 7459 10767 11803 3408 Size: 1667 2384 3308 1036 6564 Fragments arranged by size: 6564 3308 2384 1667 1036 SbfI CC_TGCA'GG Cuts at: 4143 12311 4143 Size: 8168 6791 ScaI AGT'ACT Cuts at: 7171 10351 13207 14919 7171 Size: 3180 2856 1712 7211 Fragments arranged by size: 7211 3180 2856 1712 SexAI A'CCwGG_T Cuts at: 9058 9058 Size: 14959 SfcI C'TryA_G Cuts at: 826 1045 2142 3717 4097 4139 4209 5760 7125 Size: 219 1097 1575 380 42 70 1551 1365 Cuts at: 7125 7173 8380 9198 9874 12307 13442 14123 14314 Size: 48 1207 818 676 2433 1135 681 191 Cuts at: 14314 826 Size: 1471 Fragments arranged by size: 2433 1575 1551 1471 1365 1207 1135 1097 818 681 676 380 219 191 70 48 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 14959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 14959 SmaI CCC'GGG Cuts at: 1872 12286 1872 Size: 10414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6189 6850 10754 11824 13063 13964 Size: 197 926 4246 661 3904 1070 1239 901 Cuts at: 13964 14226 820 Size: 262 1553 Fragments arranged by size: 4246 3904 1553 1239 1070 926 901 661 262 197 SnaBI TAC'GTA Cuts at: 493 8105 9630 12750 493 Size: 7612 1525 3120 2702 Fragments arranged by size: 7612 3120 2702 1525 SpeI A'CTAG_T Cuts at: 152 5123 6511 6662 7358 10127 152 Size: 4971 1388 151 696 2769 4984 Fragments arranged by size: 4984 4971 2769 1388 696 151 SphI G_CATG'C Cuts at: 1132 11561 12317 1132 Size: 10429 756 3774 Fragments arranged by size: 10429 3774 756 SspI AAT'ATT Cuts at: 5 52 2244 3271 4441 5743 5896 6310 7095 Size: 47 2192 1027 1170 1302 153 414 785 Cuts at: 7095 9280 11509 12883 5 Size: 2185 2229 1374 2081 Fragments arranged by size: 2229 2192 2185 2081 1374 1302 1170 1027 785 414 153 47 StuI AGG'CCT Cuts at: 1517 6719 11406 1517 Size: 5202 4687 5070 Fragments arranged by size: 5202 5070 4687 StyI C'CwwG_G Cuts at: 513 1065 3156 4329 4446 4782 5154 10857 11814 Size: 552 2091 1173 117 336 372 5703 957 Cuts at: 11814 11906 12398 513 Size: 92 492 3074 Fragments arranged by size: 5703 3074 2091 1173 957 552 492 372 336 117 92 SwaI ATTT'AAAT Cuts at: 8395 8395 Size: 14959 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2623 4236 10164 11950 12986 13003 13156 Size: 1255 1043 1613 5928 1786 1036 17 153 Cuts at: 13156 13341 13786 325 Size: 185 445 1498 Fragments arranged by size: 5928 1786 1613 1498 1255 1043 1036 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2447 4225 5423 Size: 274 80 33 51 1443 470 1778 1198 Cuts at: 5423 6708 6919 7169 8180 10298 10349 11597 11864 Size: 1285 211 250 1011 2118 51 1248 267 Cuts at: 11864 12161 13205 14917 96 Size: 297 1044 1712 138 Fragments arranged by size: 2118 1778 1712 1443 1285 1248 1198 1044 1011 470 297 274 267 250 211 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2550 2582 4136 4168 7531 7563 8499 8531 14919 Size: 32 1554 32 3363 32 936 32 6388 Cuts at: 14919 14951 2550 Size: 32 2558 Fragments arranged by size: 6388 3363 2558 1554 936 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 14959 XbaI T'CTAG_A Cuts at: 4016 4361 7726 8297 9851 12295 4016 Size: 345 3365 571 1554 2444 6680 Fragments arranged by size: 6680 3365 2444 1554 571 345 XcmI CCAnnnn_n'nnnnTGG Cuts at: 7811 7811 Size: 14959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 14959 XmaI C'CCGG_G Cuts at: 1870 12284 1870 Size: 10414 4545 XmnI GAAnn'nnTTC Cuts at: 3943 5038 7039 13088 3943 Size: 1095 2001 6049 5814 Fragments arranged by size: 6049 5814 2001 1095 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 12767 276 Size: 53 83 186 936 11233 2468 Fragments arranged by size: 11233 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI