################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 157 GTCTAC GT^[AC][GT]AC AluI 121 AGCT AG^CT AlwI 3 GGATC GGATC(4/5) Asp700I 66 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 303 CCTAGG C^CTAGG BalI 35 TGGCCA TGG^CCA BfaI 304 CTAG C^TAG BlnI 303 CCTAGG C^CTAGG BsaWI 133 ACCGGA [AT]^CCGG[AT] BseRI 117 GAGGAG GAGGAG(10/8) BsrBI 151 GAGCGG CCGCTC(-3/-3) BsrDI 20 GCAATG GCAATG(2/0) BssKI 328 CCTGG ^CC[ACGT]GG BstEII 298 GGTTACC G^GT[ACGT]ACC BstNI 328 CCTGG CC^[AT]GG BstPI 298 GGTTACC G^GT[ACGT]ACC Cac8I 382 GCTGGC GC[ACGT]^[ACGT]GC DraII 103 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 35 TGGCCA [CT]^GGCC[GA] EarI 358 CTCTTC CTCTTC(1/4) EcoO109I 103 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 298 GGTTACC G^GT[ACGT]ACC EcoRII 328 CCTGG ^CC[AT]GG EcoT14I 303 CCTAGG C^C[AT][AT]GG HaeIII 36 GGCC GG^CC HapII 134 CCGG C^CGG HinfI 71 GATTC G^A[ACGT]TC HpaII 134 CCGG C^CGG HphI 44 TCACC GGTGA(8/7) Ksp632I 358 CTCTTC CTCTTC(1/4) MaeI 304 CTAG C^TAG MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MslI 51 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 134 CCGG C^CGG MvaI 328 CCTGG CC^[AT]GG NlaIII 372 CATG CATG^ NlaIV 104 GGACCC GG[ACGT]^[ACGT]CC PflMI 81 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 103 AGGACCC [GA]G^G[AT]CC[CT] ScaI 287 AGTACT AGT^ACT ScrFI 328 CCTGG CC^[ACGT]GG SfcI 14 CTGTAG C^T[GA][CT]AG StyI 303 CCTAGG C^C[AT][AT]GG TfiI 71 GATTC G^A[AT]TC TspRI 162 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 81 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 66 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 153 GCGG CCGC(-3/-1) 220 GCGG BbvI 233 GCAGC GCAGC(8/12) 247 GCTGC BsaJI 303 CCTAGG C^C[ACGT][ACGT]GG 327 CCCTGG DpnII 4 GATC ^GATC 447 GATC FauI 153 GCGGG CCCGC(4/6) 220 GCGGG Fnu4HI 233 GCAGC GC^[ACGT]GC 247 GCTGC ItaI 233 GCAGC GC^[ACGT]GC 247 GCTGC MboI 4 GATC ^GATC 447 GATC MboII 359 TCTTC GAAGA(8/7) 407 TCTTC MwoI 247 GCTGCGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 323 GCACCCCTGGC NdeII 4 GATC ^GATC 447 GATC Sau3AI 4 GATC ^GATC 447 GATC SfaNI 184 GATGC GCATC(5/9) 350 GCATC TseI 233 GCAGC G^C[AT]GC 247 GCTGC Tsp45I 43 GTCAC ^GT[GC]AC 422 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AfaI AspHI AvaII BmyI BsiHKAI BsiYI BslI Bsp1286I Cfr13I MaeIII MnlI RsaI Sau96I