################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AflIII 2148 ACGTGT A^C[GA][CT]GT Alw44I 736 GTGCAC G^TGCAC ApaLI 736 GTGCAC G^TGCAC BpmI 640 CTGGAG CTGGAG(16/14) BsaAI 2428 CACGTA [CT]AC^GT[GA] BspHI 2901 TCATGA T^CATGA BsrBI 508 GAGCGG CCGCTC(-3/-3) BstAPI 3208 GCATTAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2744 TTCGAA TT^CGAA BstEII 1128 GGTCACC G^GT[ACGT]ACC BstPI 1128 GGTCACC G^GT[ACGT]ACC DraI 2395 TTTAAA TTT^AAA DraII 2490 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 2490 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1128 GGTCACC G^GT[ACGT]ACC EcoRV 63 GATATC GAT^ATC EcoT14I 503 CCTTGG C^C[AT][AT]GG EcoT22I 1301 ATGCAT ATGCA^T FauI 2727 CCCGC CCCGC(4/6) HgaI 2095 GACGC GACGC(5/10) NdeI 1114 CATATG CA^TATG NsiI 1301 ATGCAT ATGCA^T NspV 2744 TTCGAA TT^CGAA PstI 223 CTGCAG CTGCA^G RcaI 2901 TCATGA T^CATGA SacI 2579 GAGCTC GAGCT^C SfuI 2744 TTCGAA TT^CGAA SphI 2857 GCATGC GCATG^C StyI 503 CCTTGG C^C[AT][AT]GG XbaI 2757 TCTAGA T^CTAGA XcmI 2592 CCACAGTTCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 559 GATCC GGATC(4/5) 219 GGATC AlwNI 47 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG 2233 CAGTCTCTG BanI 1157 GGCACC G^G[CT][GA]CC 2766 GGTGCC BanII 243 GGGCTC G[GA]GC[CT]^C 2579 GAGCTC BbvCI 1619 CCTCAGC CCTCAGC(-5/-2) 3203 CCTCAGC BciVI 719 GTATCC GTATCC(6/5) 2431 GTATCC BsaBI 614 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3253 GATGAATATC BsaI 913 GAGACC GGTCTC(1/5) 2780 GGTCTC BseRI 2465 CTCCTC GAGGAG(10/8) 3201 CTCCTC BsmFI 49 GGGAC GGGAC(10/14) 1070 GGGAC BstYI 219 GGATCT [GA]^GATC[CT] 558 AGATCC DraIII 76 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 2315 CACTCTGTG EcoRI 1853 GAATTC G^AATTC 3028 GAATTC HindIII 1811 AAGCTT A^AGCTT 1927 AAGCTT MaeII 2148 ACGT A^CGT 2429 ACGT MamI 614 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3253 GATGAATATC MflI 219 GGATCT [GA]^GATC[CT] 558 AGATCC NlaIV 1157 GGCACC GG[ACGT]^[ACGT]CC 2766 GGTGCC SapI 318 GAAGAGC GCTCTTC(1/4) 1047 GCTCTTC SspI 897 AATATT AAT^ATT 1744 AATATT XhoII 219 GGATCT [GA]^GATC[CT] 558 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I