(Circular) (Six-base) MAPSORT of: pFN21AE5824.seq Check: 2992 from: 1 to: 7981 pFN21AE5824 7981 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5791 278 Size: 53 83 186 936 4255 2468 Fragments arranged by size: 4255 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5324 1937 Size: 3387 4594 Acc65I G'GTAC_C Cuts at: 5302 5302 Size: 7981 AclI AA'CG_TT Cuts at: 1563 6108 6481 1563 Size: 4545 373 3063 Fragments arranged by size: 4545 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2945 3456 3834 3891 4353 4530 4608 6044 Size: 1313 511 378 57 462 177 78 1436 Cuts at: 6044 7428 7696 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1436 1384 1313 511 462 378 268 177 78 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7784 AflIII A'CryG_T Cuts at: 1203 4153 6880 7857 1203 Size: 2950 2727 977 1327 Fragments arranged by size: 2950 2727 1327 977 AgeI A'CCGG_T Cuts at: 6812 6812 Size: 7981 AhdI GACnn_n'nnGTC Cuts at: 6710 6710 Size: 7981 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3321 1184 Size: 2137 5844 AlwNI CAG_nnn'CTG Cuts at: 2057 4243 5724 7296 2057 Size: 2186 1481 1572 2742 Fragments arranged by size: 2742 2186 1572 1481 ApaLI G'TGCA_C Cuts at: 2741 6038 7194 2741 Size: 3297 1156 3528 Fragments arranged by size: 3528 3297 1156 ApoI r'AATT_y Cuts at: 1493 2104 2365 2946 3455 3808 3858 4914 5033 Size: 611 261 581 509 353 50 1056 119 Cuts at: 5033 5290 5516 5668 7777 1493 Size: 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 611 581 509 353 261 257 226 152 119 50 AseI AT'TA_AT Cuts at: 160 1032 6535 160 Size: 872 5503 1606 Fragments arranged by size: 5503 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7981 AvaI C'yCGr_G Cuts at: 1870 1943 5306 1870 Size: 73 3363 4545 Fragments arranged by size: 4545 3363 73 BamHI G'GATC_C Cuts at: 5311 5678 5311 Size: 367 7614 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3162 4771 5302 6757 618 Size: 325 217 627 1375 1609 531 1455 1842 Fragments arranged by size: 1842 1609 1455 1375 627 531 325 217 BanII G_rGCy'C Cuts at: 1391 2252 4588 5300 1391 Size: 861 2336 712 4072 Fragments arranged by size: 4072 2336 861 712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4644 4719 5183 928 Size: 971 2745 75 464 3726 Fragments arranged by size: 3726 2745 971 464 75 BbvCI CC'TCA_GC Cuts at: 3625 5209 3625 Size: 1584 6397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6172 6206 1903 Size: 34 4235 34 3678 Fragments arranged by size: 4235 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2735 4447 5873 7083 2735 Size: 1712 1426 1210 3633 Fragments arranged by size: 3633 1712 1426 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7981 BfrBI ATG'CAT Cuts at: 3308 3308 Size: 7981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6592 136 Size: 107 122 71 631 605 4920 1525 Fragments arranged by size: 4920 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7976 7976 Size: 7981 BlpI GC'TnA_GC Cuts at: 5398 5398 Size: 7981 Bme1580I G_kGCm'C Cuts at: 1790 2745 4776 6042 7198 1790 Size: 955 2031 1266 1156 2573 Fragments arranged by size: 2573 2031 1266 1156 955 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7981 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5712 6670 442 Size: 416 53 83 399 24 4295 958 1753 Fragments arranged by size: 4295 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7981 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4607 Size: 7981 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2666 6641 1239 Size: 1427 3975 2579 Fragments arranged by size: 3975 2579 1427 Bpu10I CC'TnA_GC Cuts at: 3625 5209 3625 Size: 1584 6397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6106 6971 7269 6106 Size: 865 298 6818 Fragments arranged by size: 6818 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4791 6644 882 Size: 617 1413 1879 1853 2219 Fragments arranged by size: 2219 1879 1853 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 4435 5772 493 Size: 711 3231 1337 2702 Fragments arranged by size: 3231 2702 1337 711 BsaBI GATnn'nnATC Cuts at: 2623 5262 7975 2623 Size: 2639 2713 2629 Fragments arranged by size: 2713 2639 2629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5788 6170 6805 275 Size: 53 83 186 936 4255 382 635 1451 Fragments arranged by size: 4255 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6414 6812 7086 7233 7644 6414 Size: 398 274 147 411 6751 Fragments arranged by size: 6751 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7756 Fragments arranged by size: 7756 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4461 5197 1186 Size: 3275 736 3970 Fragments arranged by size: 3970 3275 736 BseYI C'CCAG_C Cuts at: 2967 7184 7680 2967 Size: 4217 496 3268 Fragments arranged by size: 4217 3268 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5431 6192 7220 1540 Size: 275 186 3430 761 1028 2301 Fragments arranged by size: 3430 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2745 2783 4588 6042 6127 7198 2745 Size: 38 1805 1454 85 1071 3528 Fragments arranged by size: 3528 1805 1454 1071 85 38 BsmI GAATG_Cn' Cuts at: 5492 5585 5492 Size: 93 7888 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2745 2783 4588 4776 5300 6042 Size: 399 462 493 38 1805 188 524 742 Cuts at: 6042 6127 7198 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1805 1071 742 524 493 462 399 188 85 38 BspEI T'CCGG_A Cuts at: 7644 7644 Size: 7981 BspHI T'CATG_A Cuts at: 4906 5870 7600 4906 Size: 964 1730 5287 Fragments arranged by size: 5287 1730 964 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5336 844 Size: 4492 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2515 5868 1394 Size: 429 692 3353 3507 Fragments arranged by size: 3507 3353 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6476 6650 65 Size: 1183 5228 174 1396 Fragments arranged by size: 5228 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6625 6812 1700 Size: 110 4815 187 2869 Fragments arranged by size: 4815 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2621 2657 4890 96 Size: 2525 36 2233 3187 Fragments arranged by size: 3187 2525 2233 36 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7981 BssSI C'ACGA_G Cuts at: 6041 7053 6041 Size: 1012 6969 BstAPI GCAn_nnn'nTGC Cuts at: 794 5219 794 Size: 4425 3556 BstBI TT'CG_AA Cuts at: 4750 6785 4750 Size: 2035 5946 BstEII G'GTnAC_C Cuts at: 1184 3133 1184 Size: 1949 6032 BstYI r'GATC_y Cuts at: 1972 2224 2563 5311 5678 6063 6080 7532 7618 Size: 252 339 2748 367 385 17 1452 86 Cuts at: 7618 7630 7976 1972 Size: 12 346 1977 Fragments arranged by size: 2748 1977 1452 385 367 346 339 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7429 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7242 Fragments arranged by size: 7242 683 56 BtsI GCAGTG_nn' Cuts at: 2237 3098 5497 6291 6311 2237 Size: 861 2399 794 20 3907 Fragments arranged by size: 3907 2399 861 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7981 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7946 DraI TTT'AAA Cuts at: 4402 5285 4402 Size: 883 7098 DraIII CAC_nnn'GTG Cuts at: 2086 4325 2086 Size: 2239 5742 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6988 809 Size: 507 5672 1802 Fragments arranged by size: 5672 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5428 6317 7946 Size: 54 1425 50 275 18 3598 889 1629 Cuts at: 7946 8 Size: 43 Fragments arranged by size: 3598 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5428 1537 Size: 275 3616 4090 Fragments arranged by size: 4090 3616 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 2745 3059 3119 5911 1353 Size: 965 427 314 60 2792 3423 Fragments arranged by size: 3423 2792 965 427 314 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6568 6942 7088 387 Size: 1064 138 4979 374 146 1280 Fragments arranged by size: 4979 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4586 4586 Size: 7981 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2666 2945 3456 3834 3891 4353 4530 Size: 393 1034 279 511 378 57 462 177 Cuts at: 4530 4608 6044 6641 7428 7696 1239 Size: 78 1436 597 787 268 1524 Fragments arranged by size: 1524 1436 1034 787 597 511 462 393 378 279 268 177 78 57 EcoO109I rG'GnC_Cy Cuts at: 4496 4496 Size: 7981 EcoRI G'AATT_C Cuts at: 3858 5033 5290 5668 3858 Size: 1175 257 378 6171 Fragments arranged by size: 6171 1175 378 257 EcoRV GAT'ATC Cuts at: 2070 2070 Size: 7981 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3344 3344 Size: 7981 FspI TGC'GCA Cuts at: 6487 6487 Size: 7981 HaeII r_GCGC'y Cuts at: 1678 7128 1678 Size: 5450 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2435 2467 3608 Size: 32 135 32 427 32 404 32 1141 Cuts at: 3608 3640 4787 4819 6622 6654 6696 6728 1373 Size: 32 1147 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 1147 1141 427 404 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2480 2545 3745 5325 5571 669 Size: 988 281 542 65 1200 1580 246 3079 Fragments arranged by size: 3079 1580 1200 988 542 281 246 65 HindIII A'AGCT_T Cuts at: 748 3816 3932 748 Size: 3068 116 4797 Fragments arranged by size: 4797 3068 116 HpaI GTT'AAC Cuts at: 5571 5571 Size: 7981 KpnI G_GTAC'C Cuts at: 5306 5306 Size: 7981 MfeI C'AATT_G Cuts at: 2855 3199 4839 5580 2855 Size: 344 1640 741 5256 Fragments arranged by size: 5256 1640 741 344 MluI A'CGCG_T Cuts at: 7857 7857 Size: 7981 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7095 7279 1305 Size: 5790 184 2007 Fragments arranged by size: 5790 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6159 Fragments arranged by size: 6159 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 4530 4536 5256 5939 6298 6457 Size: 648 2091 1273 6 720 683 359 159 Cuts at: 6457 518 Size: 2042 Fragments arranged by size: 2091 2042 1273 720 683 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2501 3629 3698 4006 4520 5397 6074 7222 Size: 895 1128 69 308 514 877 677 1148 Cuts at: 7222 7467 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1128 895 877 677 514 308 245 69 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7429 NdeI CA'TA_TG Cuts at: 387 3120 387 Size: 2733 5248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7981 NotI GC'GGCC_GC Cuts at: 5428 5428 Size: 7981 NsiI A_TGCA'T Cuts at: 3310 3310 Size: 7981 NspI r_CATG'y Cuts at: 1132 1229 3994 4535 4866 5339 6884 1132 Size: 97 2765 541 331 473 1545 2229 Fragments arranged by size: 2765 2229 1545 541 473 331 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7981 PciI A'CATG_T Cuts at: 6880 6880 Size: 7981 PflMI CCAn_nnn'nTGG Cuts at: 1108 2045 2086 3952 1108 Size: 937 41 1866 5137 Fragments arranged by size: 5137 1866 937 41 PmeI GTTT'AAAC Cuts at: 5285 5285 Size: 7981 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6050 6082 7563 7595 6050 Size: 32 1481 32 6436 Fragments arranged by size: 6436 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7981 PsiI TTA'TAA Cuts at: 3377 5551 3377 Size: 2174 5807 PstI C_TGCA'G Cuts at: 830 2232 5333 830 Size: 1402 3101 3478 Fragments arranged by size: 3478 3101 1402 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7981 PvuII CAG'CTG Cuts at: 2501 3629 3698 4006 4520 2501 Size: 1128 69 308 514 5962 Fragments arranged by size: 5962 1128 514 308 69 SacI G_AGCT'C Cuts at: 4588 4588 Size: 7981 SalI G'TCGA_C Cuts at: 1936 5323 1936 Size: 3387 4594 SapI GCTCTTCn'nnn_ Cuts at: 2318 3059 2318 Size: 741 7240 SbfI CC_TGCA'GG Cuts at: 5333 5333 Size: 7981 ScaI AGT'ACT Cuts at: 6229 7941 6229 Size: 1712 6269 SfcI C'TryA_G Cuts at: 826 1045 2228 2571 3840 4194 5329 6464 7145 Size: 219 1183 343 1269 354 1135 1135 681 Cuts at: 7145 7336 826 Size: 191 1471 Fragments arranged by size: 1471 1269 1183 1135 1135 681 354 343 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7981 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7981 SmaI CCC'GGG Cuts at: 1872 5308 1872 Size: 3436 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6085 6986 7248 820 Size: 197 926 4142 901 262 1553 Fragments arranged by size: 4142 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5772 493 Size: 5279 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7981 SphI G_CATG'C Cuts at: 1132 4866 5339 1132 Size: 3734 473 3774 Fragments arranged by size: 3774 3734 473 SspI AAT'ATT Cuts at: 5 52 2904 3751 5905 5 Size: 47 2852 847 2154 2081 Fragments arranged by size: 2852 2154 2081 847 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7981 StyI C'CwwG_G Cuts at: 513 1065 2508 5420 513 Size: 552 1443 2912 3074 Fragments arranged by size: 3074 2912 1443 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3180 3602 4542 6008 6025 6178 6363 Size: 1255 1600 422 940 1466 17 153 185 Cuts at: 6363 6808 325 Size: 445 1498 Fragments arranged by size: 1600 1498 1466 1255 940 445 422 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2621 2657 4000 Size: 274 80 33 51 1443 644 36 1343 Cuts at: 4000 4890 6227 7939 96 Size: 890 1337 1712 138 Fragments arranged by size: 1712 1443 1343 1337 890 644 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 4858 4890 7941 7973 2216 Size: 32 2610 32 3051 32 2224 Fragments arranged by size: 3051 2610 2224 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7981 XbaI T'CTAG_A Cuts at: 4762 5317 4762 Size: 555 7426 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4604 4604 Size: 7981 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7981 XmaI C'CCGG_G Cuts at: 1870 5306 1870 Size: 3436 4545 XmnI GAAnn'nnTTC Cuts at: 6110 6110 Size: 7981 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5789 276 Size: 53 83 186 936 4255 2468 Fragments arranged by size: 4255 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI