(Linear) (Six-base) MAPSORT of: KIEE5824.seq Check: 4376 from: 1 to: 3276 KIEE5824.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 940 1451 1829 1886 2348 2525 2603 3276 Size: 940 511 378 57 462 177 78 673 Fragments arranged by size: 940 673 511 462 378 177 78 57 AflIII A'CryG_T Cuts at: 0 2148 3276 Size: 2148 1128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1316 3276 Size: 1316 1960 AlwNI CAG_nnn'CTG Cuts at: 0 52 2238 3276 Size: 52 2186 1038 Fragments arranged by size: 2186 1038 52 ApaLI G'TGCA_C Cuts at: 0 736 3276 Size: 736 2540 ApoI r'AATT_y Cuts at: 0 99 360 941 1450 1803 1853 2909 3028 Size: 99 261 581 509 353 50 1056 119 Cuts at: 3028 3276 Size: 248 Fragments arranged by size: 1056 581 509 353 261 248 119 99 50 BanI G'GyrC_C Cuts at: 0 1157 2766 3276 Size: 1157 1609 510 Fragments arranged by size: 1609 1157 510 BanII G_rGCy'C Cuts at: 0 247 2583 3276 Size: 247 2336 693 Fragments arranged by size: 2336 693 247 BbsI GAAGACnn'nnnn_ Cuts at: 0 2639 2714 3178 3276 Size: 2639 75 464 98 Fragments arranged by size: 2639 464 98 75 BbvCI CC'TCA_GC Cuts at: 0 1620 3204 3276 Size: 1620 1584 72 Fragments arranged by size: 1620 1584 72 BciVI GTATCCnnnnn_n' Cuts at: 0 730 2442 3276 Size: 730 1712 834 Fragments arranged by size: 1712 834 730 BfrBI ATG'CAT Cuts at: 0 1303 3276 Size: 1303 1973 Bme1580I G_kGCm'C Cuts at: 0 740 2771 3276 Size: 740 2031 505 Fragments arranged by size: 2031 740 505 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2602 3276 Size: 2602 674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 661 3276 Size: 661 2615 Bpu10I CC'TnA_GC Cuts at: 0 1620 3204 3276 Size: 1620 1584 72 Fragments arranged by size: 1620 1584 72 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 2786 3276 Size: 907 1879 490 Fragments arranged by size: 1879 907 490 BsaAI yAC'GTr Cuts at: 0 2430 3276 Size: 2430 846 BsaBI GATnn'nnATC Cuts at: 0 618 3257 3276 Size: 618 2639 19 Fragments arranged by size: 2639 618 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2456 3192 3276 Size: 2456 736 84 Fragments arranged by size: 2456 736 84 BseYI C'CCAG_C Cuts at: 0 962 3276 Size: 962 2314 BsiHKAI G_wGCw'C Cuts at: 0 740 778 2583 3276 Size: 740 38 1805 693 Fragments arranged by size: 1805 740 693 38 Bsp1286I G_dGCh'C Cuts at: 0 247 740 778 2583 2771 3276 Size: 247 493 38 1805 188 505 Fragments arranged by size: 1805 505 493 247 188 38 BspHI T'CATG_A Cuts at: 0 2901 3276 Size: 2901 375 BsrBI CCG'CTC Cuts at: 0 510 3276 Size: 510 2766 BsrGI T'GTAC_A Cuts at: 0 616 652 2885 3276 Size: 616 36 2233 391 Fragments arranged by size: 2233 616 391 36 BstAPI GCAn_nnn'nTGC Cuts at: 0 3214 3276 Size: 3214 62 BstBI TT'CG_AA Cuts at: 0 2745 3276 Size: 2745 531 BstEII G'GTnAC_C Cuts at: 0 1128 3276 Size: 1128 2148 BstYI r'GATC_y Cuts at: 0 219 558 3276 Size: 219 339 2718 Fragments arranged by size: 2718 339 219 BtsI GCAGTG_nn' Cuts at: 0 232 1093 3276 Size: 232 861 2183 Fragments arranged by size: 2183 861 232 DraI TTT'AAA Cuts at: 0 2397 3276 Size: 2397 879 DraIII CAC_nnn'GTG Cuts at: 0 81 2320 3276 Size: 81 2239 956 Fragments arranged by size: 2239 956 81 EarI CTCTTCn'nnn_ Cuts at: 0 313 740 1054 1114 3276 Size: 313 427 314 60 2162 Fragments arranged by size: 2162 427 314 313 60 EcoICRI GAG'CTC Cuts at: 0 2581 3276 Size: 2581 695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 661 940 1451 1829 1886 2348 2525 2603 Size: 661 279 511 378 57 462 177 78 Cuts at: 2603 3276 Size: 673 Fragments arranged by size: 673 661 511 462 378 279 177 78 57 EcoO109I rG'GnC_Cy Cuts at: 0 2491 3276 Size: 2491 785 EcoRI G'AATT_C Cuts at: 0 1853 3028 3276 Size: 1853 1175 248 Fragments arranged by size: 1853 1175 248 EcoRV GAT'ATC Cuts at: 0 65 3276 Size: 65 3211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1339 3276 Size: 1339 1937 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 26 430 462 1603 1635 2782 2814 3276 Size: 26 404 32 1141 32 1147 32 462 Fragments arranged by size: 1147 1141 462 404 32 32 32 26 HincII GTy'rAC Cuts at: 0 475 540 1740 3276 Size: 475 65 1200 1536 Fragments arranged by size: 1536 1200 475 65 HindIII A'AGCT_T Cuts at: 0 1811 1927 3276 Size: 1811 116 1349 Fragments arranged by size: 1811 1349 116 MfeI C'AATT_G Cuts at: 0 850 1194 2834 3276 Size: 850 344 1640 442 Fragments arranged by size: 1640 850 442 344 MslI CAynn'nnrTG Cuts at: 0 1252 2525 2531 3251 3276 Size: 1252 1273 6 720 25 Fragments arranged by size: 1273 1252 720 25 6 MspA1I CmG'CkG Cuts at: 0 496 1624 1693 2001 2515 3276 Size: 496 1128 69 308 514 761 Fragments arranged by size: 1128 761 514 496 308 69 NdeI CA'TA_TG Cuts at: 0 1115 3276 Size: 1115 2161 NsiI A_TGCA'T Cuts at: 0 1305 3276 Size: 1305 1971 NspI r_CATG'y Cuts at: 0 1989 2530 2861 3276 Size: 1989 541 331 415 Fragments arranged by size: 1989 541 415 331 PflMI CCAn_nnn'nTGG Cuts at: 0 40 81 1947 3276 Size: 40 41 1866 1329 Fragments arranged by size: 1866 1329 41 40 PsiI TTA'TAA Cuts at: 0 1372 3276 Size: 1372 1904 PstI C_TGCA'G Cuts at: 0 227 3276 Size: 227 3049 PvuII CAG'CTG Cuts at: 0 496 1624 1693 2001 2515 3276 Size: 496 1128 69 308 514 761 Fragments arranged by size: 1128 761 514 496 308 69 SacI G_AGCT'C Cuts at: 0 2583 3276 Size: 2583 693 SapI GCTCTTCn'nnn_ Cuts at: 0 313 1054 3276 Size: 313 741 2222 Fragments arranged by size: 2222 741 313 SfcI C'TryA_G Cuts at: 0 223 566 1835 2189 3276 Size: 223 343 1269 354 1087 Fragments arranged by size: 1269 1087 354 343 223 SphI G_CATG'C Cuts at: 0 2861 3276 Size: 2861 415 SspI AAT'ATT Cuts at: 0 899 1746 3276 Size: 899 847 1530 Fragments arranged by size: 1530 899 847 StyI C'CwwG_G Cuts at: 0 503 3276 Size: 503 2773 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1175 1597 2537 3276 Size: 1175 422 940 739 Fragments arranged by size: 1175 940 739 422 TatI w'GTAC_w Cuts at: 0 616 652 1995 2885 3276 Size: 616 36 1343 890 391 Fragments arranged by size: 1343 890 616 391 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 211 243 2853 2885 3276 Size: 211 32 2610 32 391 Fragments arranged by size: 2610 391 211 32 32 XbaI T'CTAG_A Cuts at: 0 2757 3276 Size: 2757 519 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2599 3276 Size: 2599 677 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApaLI ApoI BanI BanII BbsI BbvCI BciVI BfrBI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrBI BsrGI BstAPI BstBI BstEII BstYI BtsI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MslI MspA1I NdeI NsiI NspI PflMI PsiI PstI PvuII SacI SapI SfcI SphI SspI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BclI BglI BglII BlpI BmgBI BmrI BmtI BpuEI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrFI BssHII BssSI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI XmnI ZraI