################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccII AccIII AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 595 GTAGAC GT^[AC][GT]AC AclI 229 AACGTT AA^CGTT AfaI 167 GTAC GT^AC AflIII 599 ACGTGT A^C[GA][CT]GT AlwI 405 GATCC GGATC(4/5) AvaII 460 GGACC G^G[AT]CC BbvCI 426 GCTGAGG CCTCAGC(-5/-2) BbvI 4 GCTGC GCAGC(8/12) BsmFI 111 GTCCC GGGAC(10/14) BspHI 389 TCATGA T^CATGA BsrBI 415 GAGCGG CCGCTC(-3/-3) BsrDI 281 GCAATG GCAATG(2/0) BstYI 404 AGATCC [GA]^GATC[CT] Cfr13I 460 GGACC G^G[ACGT]CC DpnII 405 GATC ^GATC DsaI 798 CCATGG C^C[GA][CT]GG EcoNI 468 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 798 CCATGG C^C[AT][AT]GG HaeIII 677 GGCC GG^CC HincII 24 GTTAAC GT[CT]^[GA]AC HindII 24 GTTAAC GT[CT]^[GA]AC HindIII 701 AAGCTT A^AGCTT HpaI 24 GTTAAC GTT^AAC MaeIII 227 GTAAC ^GT[ACGT]AC MboI 405 GATC ^GATC MflI 404 AGATCC [GA]^GATC[CT] MslI 799 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 170 CCGCTG C[AC]G^C[GT]G NcoI 798 CCATGG C^CATGG NdeII 405 GATC ^GATC NspI 296 ACATGC [GA]CATG^[CT] PleI 134 GACTC GAGTC(4/5) Psp1406I 229 AACGTT AA^CGTT PstI 374 CTGCAG CTGCA^G RcaI 389 TCATGA T^CATGA RsaI 167 GTAC GT^AC Sau3AI 405 GATC ^GATC Sau96I 460 GGACC G^G[ACGT]CC SfaNI 828 GCATC GCATC(5/9) SfcI 374 CTGCAG C^T[GA][CT]AG SmlI 761 CTCAAG C^T[CT][GA]AG SspI 496 AATATT AAT^ATT StyI 798 CCATGG C^C[AT][AT]GG TseI 4 GCTGC G^C[AT]GC TspRI 541 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 642 CCAGACTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 404 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 170 CCGC CCGC(-3/-1) 417 GCGG AlwNI 368 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 421 CAGAGGCTG BbsI 175 GAAGAC GAAGAC(2/6) 489 GTCTTC BfaI 562 CTAG C^TAG 674 CTAG BpmI 409 CTCCAG CTGGAG(16/14) 640 CTCCAG BpuAI 175 GAAGAC GAAGAC(2/6) 489 GTCTTC BsaI 335 GAGACC GGTCTC(1/5) 766 GAGACC BsaJI 770 CCCAGG C^C[ACGT][ACGT]GG 798 CCATGG BseRI 619 GAGGAG GAGGAG(10/8) 739 GAGGAG BspMI 338 ACCTGC ACCTGC(4/8) 372 ACCTGC BsrI 345 ACTGG ACTGG(1/-1) 457 ACTGG BstF5I 81 CATCC GGATG(2/0) 327 GGATG DdeI 427 CTGAG C^T[ACGT]AG 469 CTGAG Fnu4HI 4 GCTGC GC^[ACGT]GC 417 GCGGC FokI 81 CATCC GGATG(9/13) 327 GGATG ItaI 4 GCTGC GC^[ACGT]GC 417 GCGGC MaeI 562 CTAG C^TAG 674 CTAG MaeII 230 ACGT A^CGT 599 ACGT MwoI 333 GCGAGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 417 GCGGCAGAGGC TfiI 534 GATTC G^A[AT]TC 814 GAATC Tsp509I 147 AATT ^AATT 243 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BsiYI BslI BsmAI BssKI BstNI EcoRII HinfI MboII MnlI MseI MvaI NlaIII ScrFI Tru9I