(Circular) (Six-base) MAPSORT of: pFN21AE5326.seq Check: 4538 from: 1 to: 5539 pFN21AE5326 5539 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2601 2882 1937 Size: 664 281 4594 Fragments arranged by size: 4594 664 281 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 2235 3666 4039 1563 Size: 672 1431 373 3063 Fragments arranged by size: 3063 1431 672 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 3602 4986 5254 1632 Size: 567 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 567 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 2604 4438 5415 1203 Size: 1401 1834 977 1327 Fragments arranged by size: 1834 1401 1327 977 AgeI A'CCGG_T Cuts at: 4370 4370 Size: 5539 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 2378 2431 3282 4854 2378 Size: 53 851 1572 3063 Fragments arranged by size: 3063 1572 851 53 ApaLI G'TGCA_C Cuts at: 3596 4752 3596 Size: 1156 4383 ApoI r'AATT_y Cuts at: 1493 2848 3074 3226 5335 1493 Size: 1355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 226 152 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2864 1870 Size: 73 921 4545 Fragments arranged by size: 4545 921 73 BamHI G'GATC_C Cuts at: 2869 3236 2869 Size: 367 5172 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2860 4315 618 Size: 325 217 627 1073 1455 1842 Fragments arranged by size: 1842 1455 1073 627 325 217 BanII G_rGCy'C Cuts at: 1391 2858 1391 Size: 1467 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2187 2487 928 Size: 971 288 300 3980 Fragments arranged by size: 3980 971 300 288 BbvCI CC'TCA_GC Cuts at: 2432 2432 Size: 5539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 1903 Size: 34 1793 34 3678 Fragments arranged by size: 3678 1793 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5534 5534 Size: 5539 BlpI GC'TnA_GC Cuts at: 2956 2956 Size: 5539 Bme1580I G_kGCm'C Cuts at: 1790 3600 4756 1790 Size: 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4228 442 Size: 416 53 83 399 24 1853 958 1753 Fragments arranged by size: 1853 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 2630 4199 1239 Size: 1160 231 1569 2579 Fragments arranged by size: 2579 1569 1160 231 Bpu10I CC'TnA_GC Cuts at: 2432 2432 Size: 5539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2751 3664 4529 4827 2751 Size: 913 865 298 3463 Fragments arranged by size: 3463 913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2334 2765 4202 882 Size: 617 835 431 1437 2219 Fragments arranged by size: 2219 1437 835 617 431 BsaAI yAC'GTr Cuts at: 493 1204 3330 493 Size: 711 2126 2702 Fragments arranged by size: 2702 2126 711 BsaBI GATnn'nnATC Cuts at: 5533 5533 Size: 5539 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3346 3728 4363 275 Size: 53 83 186 936 1813 382 635 1451 Fragments arranged by size: 1813 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 4370 4644 4791 5202 3972 Size: 398 274 147 411 4309 Fragments arranged by size: 4309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2639 2759 1186 Size: 1453 120 3966 Fragments arranged by size: 3966 1453 120 BseYI C'CCAG_C Cuts at: 4742 5238 4742 Size: 496 5043 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4966 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2989 3750 4778 1540 Size: 275 186 988 761 1028 2301 Fragments arranged by size: 2301 1028 988 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3600 3685 4756 3600 Size: 85 1071 4383 Fragments arranged by size: 4383 1071 85 BsmI GAATG_Cn' Cuts at: 3050 3143 3050 Size: 93 5446 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2858 3600 3685 4756 1391 Size: 399 1068 742 85 1071 2174 Fragments arranged by size: 2174 1071 1068 742 399 85 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 2394 3428 5158 2394 Size: 1034 1730 2775 Fragments arranged by size: 2775 1730 1034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2386 2894 844 Size: 1508 34 508 3489 Fragments arranged by size: 3489 1508 508 34 BsrBI CCG'CTC Cuts at: 1394 1823 2422 3426 1394 Size: 429 599 1004 3507 Fragments arranged by size: 3507 1004 599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 4034 4208 65 Size: 1183 1045 1741 174 1396 Fragments arranged by size: 1741 1396 1183 1045 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4183 4370 1700 Size: 110 2373 187 2869 Fragments arranged by size: 2869 2373 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5539 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5539 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5539 BstBI TT'CG_AA Cuts at: 4343 4343 Size: 5539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstYI r'GATC_y Cuts at: 1972 2409 2869 3236 3621 3638 5090 5176 5188 Size: 437 460 367 385 17 1452 86 12 Cuts at: 5188 5534 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 460 437 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2803 513 Size: 552 939 799 3249 Fragments arranged by size: 3249 939 799 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4800 Fragments arranged by size: 4800 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3055 3849 3869 2552 Size: 503 794 20 4222 Fragments arranged by size: 4222 794 503 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5504 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504 Size: 54 1425 50 275 18 1156 889 1629 Cuts at: 5504 8 Size: 43 Fragments arranged by size: 1629 1425 1156 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2986 1537 Size: 275 1174 4090 Fragments arranged by size: 4090 1174 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4126 4500 4646 387 Size: 1064 138 2537 374 146 1280 Fragments arranged by size: 2537 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2399 2630 3602 4199 4986 5254 Size: 393 567 200 231 972 597 787 268 Cuts at: 5254 1239 Size: 1524 Fragments arranged by size: 1524 972 787 597 567 393 268 231 200 EcoNI CCTnn'n_nnAGG Cuts at: 2477 2477 Size: 5539 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2805 2805 Size: 5539 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 4686 1678 Size: 3008 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2394 2426 2620 2652 2804 Size: 32 135 32 822 32 194 32 152 Cuts at: 2804 2836 4180 4212 4254 4286 1373 Size: 32 1344 32 42 32 2626 Fragments arranged by size: 2626 1344 822 194 152 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2031 2883 3129 669 Size: 988 281 93 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 93 HindIII A'AGCT_T Cuts at: 748 2706 748 Size: 1958 3581 HpaI GTT'AAC Cuts at: 2031 3129 2031 Size: 1098 4441 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2218 4653 4837 1305 Size: 913 2435 184 2007 Fragments arranged by size: 2435 2007 913 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2808 3497 3856 4015 518 Size: 648 1642 689 359 159 2042 Fragments arranged by size: 2042 1642 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2177 2955 3632 4780 5025 1606 Size: 571 778 677 1148 245 2120 Fragments arranged by size: 2120 1148 778 677 571 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2803 513 Size: 552 939 799 3249 Fragments arranged by size: 3249 939 799 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2305 2897 4442 1132 Size: 97 1076 592 1545 2229 Fragments arranged by size: 2229 1545 1076 592 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5539 PfoI T'CCnGG_A Cuts at: 2415 2415 Size: 5539 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PstI C_TGCA'G Cuts at: 830 2383 2891 830 Size: 1553 508 3478 Fragments arranged by size: 3478 1553 508 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SfcI C'TryA_G Cuts at: 826 1045 2379 2887 4022 4703 4894 826 Size: 219 1334 508 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 681 508 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5539 SmaI CCC'GGG Cuts at: 1872 2866 1872 Size: 994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2766 3643 4544 4806 820 Size: 197 926 823 877 901 262 1553 Fragments arranged by size: 1553 926 901 877 823 262 197 SnaBI TAC'GTA Cuts at: 493 3330 493 Size: 2837 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 2503 3463 5 Size: 47 2451 960 2081 Fragments arranged by size: 2451 2081 960 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5539 StyI C'CwwG_G Cuts at: 513 1065 2004 2803 2978 513 Size: 552 939 799 175 3074 Fragments arranged by size: 3074 939 799 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 3566 3583 3736 3921 4366 325 Size: 1255 621 1365 17 153 185 445 1498 Fragments arranged by size: 1498 1365 1255 621 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3785 5497 96 Size: 274 80 33 51 1443 1808 1712 138 Fragments arranged by size: 1808 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5499 5531 5499 Size: 32 5507 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5539 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2654 2654 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5539 XmaI C'CCGG_G Cuts at: 1870 2864 1870 Size: 994 4545 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI