################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AlwI 921 GATCC GGATC(4/5) AlwNI 113 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 930 AGCGCT AGC^GCT AspI 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 713 TGGCCA TGG^CCA BanII 121 GAGCTC G[GA]GC[CT]^C BbsI 7 GAAGAC GAAGAC(2/6) BglII 656 AGATCT A^GATCT BpuAI 7 GAAGAC GAAGAC(2/6) BsaI 747 GGTCTC GGTCTC(1/5) BseRI 87 CTCCTC GAGGAG(10/8) BsiEI 347 CGATCG CG[GA][CT]^CG BsmBI 548 GAGACG CGTCTC(1/5) BsrBI 577 CCGCTC CCGCTC(-3/-3) BsrFI 277 GCCGGC [GA]^CCGG[CT] BssHII 982 GCGCGC G^CGCGC Cfr10I 277 GCCGGC [GA]^CCGG[CT] DdeI 119 CTGAG C^T[ACGT]AG DraII 496 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 724 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 10 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 619 CTCTTC CTCTTC(1/4) Eco47III 930 AGCGCT AGC^GCT EcoO109I 496 GGGACCT [GA]G^G[ACGT]CC[CT] HaeII 930 AGCGCT [GA]GCGC^[CT] Ksp632I 619 CTCTTC CTCTTC(1/4) MluNI 713 TGGCCA TGG^CCA MscI 713 TGGCCA TGG^CCA MseI 25 TTAA T^TAA MspA1I 56 CCGCTG C[AC]G^C[GT]G NaeI 277 GCCGGC GCC^GGC NgoMIV 277 GCCGGC G^CCGGC PflFI 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 705 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 496 GGGACCT [GA]G^G[AT]CC[CT] PvuI 347 CGATCG CGAT^CG SacI 121 GAGCTC GAGCT^C SmaI 92 CCCGGG CCC^GGG Tru9I 25 TTAA T^TAA Tth111I 142 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 705 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 557 CCACCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 92 CCCGGG C^CCGGG XmaI 92 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 594 CGCG CG^CG 983 CGCG AspHI 121 GAGCTC G[AT]GC[AT]^C 227 GAGCAC AvaII 195 GGTCC G^G[AT]CC 497 GGACC BmyI 121 GAGCTC G[AGT]GC[ACT]^C 227 GAGCAC BsiHKAI 121 GAGCTC G[AT]GC[AT]^C 227 GAGCAC Bsp1286I 121 GAGCTC G[AGT]GC[ACT]^C 227 GAGCAC BsrI 965 CCAGT ACTGG(1/-1) 992 CCAGT BstUI 594 CGCG CG^CG 983 CGCG BstXI 332 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 503 CCATCTGCCTGG BstYI 656 AGATCT [GA]^GATC[CT] 920 AGATCC Cfr13I 195 GGTCC G^G[ACGT]CC 497 GGACC FauI 865 CCCGC CCCGC(4/6) 29 GCGGG HincII 130 GTCAAC GT[CT]^[GA]AC 406 GTTGAC HindII 130 GTCAAC GT[CT]^[GA]AC 406 GTTGAC HinfI 287 GACTC G^A[ACGT]TC 553 GAGTC MflI 656 AGATCT [GA]^GATC[CT] 920 AGATCC MvnI 594 CGCG CG^CG 983 CGCG PleI 287 GACTC GAGTC(4/5) 553 GAGTC Sau96I 195 GGTCC G^G[ACGT]CC 497 GGACC XhoII 656 AGATCT [GA]^GATC[CT] 920 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AvaI BbvI BcnI BsaJI BsiYI BslI BsmAI BsmFI BsoBI BspMI BssKI BstF5I BstNI Cac8I CfoI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI