################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspHI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BcnI BglI BglII BlnI BpmI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CfoI ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 593 GGTACC G^GTACC AcsI 1069 GAATTT [GA]^AATT[CT] AflII 217 CTTAAG C^TTAAG AlwNI 937 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1235 CCTCAGG CC^T[ACGT]AGG ApoI 1069 GAATTT [GA]^AATT[CT] AseI 834 ATTAAT AT^TAAT AsnI 834 ATTAAT AT^TAAT Asp718I 593 GGTACC G^GTACC AvaI 371 CTCGGG C^[CT]CG[GA]G BalI 1155 TGGCCA TGG^CCA BamHI 570 GGATCC G^GATCC BanI 593 GGTACC G^G[CT][GA]CC BanII 599 GAGCCC G[GA]GC[CT]^C BbsI 1009 GAAGAC GAAGAC(2/6) BciVI 1336 GGATAC GTATCC(6/5) BfrI 217 CTTAAG C^TTAAG BlpI 24 GCTGAGC GC^T[ACGT]AGC BmyI 599 GAGCCC G[AGT]GC[ACT]^C Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC BpuAI 1009 GAAGAC GAAGAC(2/6) BsaAI 858 TACGTA [CT]AC^GT[GA] BsaBI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 1191 GGGAC GGGAC(10/14) BsmI 922 GCATTC GAATGC(1/-1) BsoBI 371 CTCGGG C^[CT]CG[GA]G Bsp1286I 599 GAGCCC G[AGT]GC[ACT]^C BspHI 1250 TCATGA T^CATGA BsrFI 1039 GCCGGT [GA]^CCGG[CT] Bst1107I 861 GTATAC GTA^TAC BstXI 1140 CCACACTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 861 GTATAC GTA^TAC Bsu36I 1235 CCTCAGG CC^T[ACGT]AGG CelII 24 GCTGAGC GC^T[ACGT]AGC Cfr10I 1039 GCCGGT [GA]^CCGG[CT] DsaI 950 CCATGG C^C[GA][CT]GG Eco81I 1235 CCTCAGG CC^T[ACGT]AGG EcoNI 490 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 950 CCATGG C^C[AT][AT]GG HapII 1040 CCGG C^CGG HincII 707 GTCGAC GT[CT]^[GA]AC HindII 707 GTCGAC GT[CT]^[GA]AC HinfI 1167 GAATC G^A[ACGT]TC HpaII 1040 CCGG C^CGG KpnI 593 GGTACC GGTAC^C MamI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1155 TGGCCA TGG^CCA MscI 1155 TGGCCA TGG^CCA MslI 1141 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 751 CAGCTG C[AC]G^C[GT]G MspI 1040 CCGG C^CGG NcoI 950 CCATGG C^CATGG PshAI 1174 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 834 ATTAAT AT^TAAT PvuII 751 CAGCTG CAG^CTG RcaI 1250 TCATGA T^CATGA SalI 707 GTCGAC G^TCGAC ScaI 1367 AGTACT AGT^ACT SmlI 217 CTTAAG C^T[CT][GA]AG SnaBI 858 TACGTA TAC^GTA SpeI 727 ACTAGT A^CTAGT SphI 426 GCATGC GCATG^C StyI 950 CCATGG C^C[AT][AT]GG TfiI 1167 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 607 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 840 GAAAGATTTC BfaI 406 CTAG C^TAG 728 CTAG BsiYI 490 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1294 CCCAACCCTGG BslI 490 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1294 CCCAACCCTGG BsmAI 1103 GAGAC GTCTC(1/5) 258 GTCTC BspMI 422 ACCTGC ACCTGC(4/8) 459 ACCTGC BsrBI 34 GAGCGG CCGCTC(-3/-3) 40 GAGCGG BssKI 1162 CCAGG ^CC[ACGT]GG 1300 CCTGG BstF5I 223 CATCC GGATG(2/0) 132 GGATG BstNI 1162 CCAGG CC^[AT]GG 1300 CCTGG Cac8I 32 GCGAGC GC[ACGT]^[ACGT]GC 426 GCATGC DraI 13 TTTAAA TTT^AAA 150 TTTAAA EaeI 81 TGGCCG [CT]^GGCC[GA] 1155 TGGCCA EarI 239 CTCTTC CTCTTC(1/4) 500 GAAGAG EcoRII 1162 CCAGG ^CC[AT]GG 1300 CCTGG FauI 36 GCGGG CCCGC(4/6) 42 GCGGG FokI 223 CATCC GGATG(9/13) 132 GGATG HaeIII 82 GGCC GG^CC 1156 GGCC Ksp632I 239 CTCTTC CTCTTC(1/4) 500 GAAGAG MaeI 406 CTAG C^TAG 728 CTAG MvaI 1162 CCAGG CC^[AT]GG 1300 CCTGG PstI 785 CTGCAG CTGCA^G 870 CTGCAG ScrFI 1162 CCAGG CC^[ACGT]GG 1300 CCTGG SfaNI 816 GATGC GCATC(5/9) 222 GCATC TspRI 575 CAGTG CA[GC]TG[ACGT][ACGT]^ 703 CACTG XmnI 607 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 840 GAAAGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AvaII BbvI BsaJI BsrI BstYI Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MwoI NdeII NlaIII NlaIV NspI PpuMI RsaI Sau3AI Sau96I SfcI SspI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII