(Circular) (Six-base) MAPSORT of: pFN21AB5871.seq Check: 2927 from: 1 to: 6097 pFN21AB5871 6097 bp With 182 enzymes: SgfI * March 28, 2012 12:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2713 2867 3440 1937 Size: 172 604 154 573 4594 Fragments arranged by size: 4594 604 573 172 154 Acc65I G'GTAC_C Cuts at: 2598 3418 2598 Size: 820 5277 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 4160 5544 5812 1632 Size: 1292 1236 1384 268 1917 Fragments arranged by size: 1917 1384 1292 1236 268 AflII C'TTAA_G Cuts at: 820 1017 2222 820 Size: 197 1205 4695 Fragments arranged by size: 4695 1205 197 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 2947 3840 5412 2947 Size: 893 1572 3632 Fragments arranged by size: 3632 1572 893 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 3074 3406 3632 3784 5893 1493 Size: 1581 332 226 152 2109 1697 Fragments arranged by size: 2109 1697 1581 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 2840 4651 160 Size: 872 1808 1811 1606 Fragments arranged by size: 1811 1808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2376 3422 1870 Size: 73 433 1046 4545 Fragments arranged by size: 4545 1046 433 73 BamHI G'GATC_C Cuts at: 2575 3427 3794 2575 Size: 852 367 4878 Fragments arranged by size: 4878 852 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2598 3418 4873 618 Size: 325 217 627 811 820 1455 1842 Fragments arranged by size: 1842 1455 820 811 627 325 217 BanII G_rGCy'C Cuts at: 1391 2608 3416 1391 Size: 1217 808 4072 Fragments arranged by size: 4072 1217 808 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 928 Size: 971 1122 4004 Fragments arranged by size: 4004 1122 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 3989 5199 3335 Size: 654 1210 4233 Fragments arranged by size: 4233 1210 654 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 2030 3514 2030 Size: 1484 4613 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 2107 1318 Size: 789 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 2449 3828 Size: 416 53 83 399 24 767 265 1379 Cuts at: 3828 4786 442 Size: 958 1753 Fragments arranged by size: 1753 1379 958 767 416 399 265 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4757 1239 Size: 3518 2579 Bpu10I CC'TnA_GC Cuts at: 2496 2496 Size: 6097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2865 3888 493 Size: 711 1661 1023 2702 Fragments arranged by size: 2702 1661 1023 711 BsaBI GATnn'nnATC Cuts at: 2574 6091 2574 Size: 3517 2580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5872 Fragments arranged by size: 5872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6097 BseYI C'CCAG_C Cuts at: 3357 5300 5796 3357 Size: 1943 496 3658 Fragments arranged by size: 3658 1943 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4158 4243 5314 4158 Size: 85 1071 4941 Fragments arranged by size: 4941 1071 85 BsmI GAATG_Cn' Cuts at: 2927 3608 3701 2927 Size: 681 93 5323 Fragments arranged by size: 5323 681 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2608 3416 4158 4243 5314 1391 Size: 399 818 808 742 85 1071 2174 Fragments arranged by size: 2174 1071 818 808 742 399 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3255 3986 5716 3255 Size: 731 1730 3636 Fragments arranged by size: 3636 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2436 2473 3452 844 Size: 1592 37 979 3489 Fragments arranged by size: 3489 1592 979 37 BsrBI CCG'CTC Cuts at: 1394 1823 2041 2047 3984 1394 Size: 429 218 6 1937 3507 Fragments arranged by size: 3507 1937 429 218 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3044 4741 4928 1700 Size: 110 1234 1697 187 2869 Fragments arranged by size: 2869 1697 1234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstXI CCAn_nnnn'nTGG Cuts at: 3152 3152 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2178 2406 2575 3427 3794 4179 4196 5648 Size: 206 228 169 852 367 385 17 1452 Cuts at: 5648 5734 5746 6092 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 852 385 367 346 228 206 169 86 17 12 BstZ17I GTA'TAC Cuts at: 2868 2868 Size: 6097 Bsu36I CC'TnA_GG Cuts at: 3241 3241 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2004 2955 513 Size: 552 939 951 3655 Fragments arranged by size: 3655 951 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 2020 2157 3401 2020 Size: 137 1244 4716 Fragments arranged by size: 4716 1244 137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 3160 3544 Size: 54 1425 50 275 18 256 1074 384 Cuts at: 3544 4433 6062 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1074 889 384 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2500 4027 1353 Size: 897 250 1527 3423 Fragments arranged by size: 3423 1527 897 250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 4160 4757 5544 5812 1239 Size: 393 1292 1236 597 787 268 1524 Fragments arranged by size: 1524 1292 1236 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2499 2499 Size: 6097 EcoO109I rG'GnC_Cy Cuts at: 2056 2492 2998 3197 2056 Size: 436 506 199 4956 Fragments arranged by size: 4956 506 436 199 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3038 3038 Size: 6097 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373 Size: 32 135 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2714 3441 3687 669 Size: 988 281 776 727 246 3079 Fragments arranged by size: 3079 988 776 727 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 2602 3422 2602 Size: 820 5277 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3162 10 Size: 54 1425 343 1330 2945 Fragments arranged by size: 2945 1425 1330 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3150 4055 4414 4573 518 Size: 648 1984 905 359 159 2042 Fragments arranged by size: 2042 1984 905 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2758 3513 4190 5338 5583 1606 Size: 1152 755 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 755 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2955 513 Size: 552 939 951 3655 Fragments arranged by size: 3655 951 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2196 2435 3290 3455 5000 1132 Size: 97 967 239 855 165 1545 2229 Fragments arranged by size: 2229 1545 967 855 239 165 97 PasI CC'CwG_GG Cuts at: 1348 3167 1348 Size: 1819 4278 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2298 2330 4166 4198 5679 5711 2298 Size: 32 1836 32 1481 32 2684 Fragments arranged by size: 2684 1836 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2492 2998 3197 2056 Size: 436 506 199 4956 Fragments arranged by size: 4956 506 436 199 PshAI GACnn'nnGTC Cuts at: 1613 3183 1613 Size: 1570 4527 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PstI C_TGCA'G Cuts at: 830 2794 2879 3449 830 Size: 1964 85 570 3478 Fragments arranged by size: 3478 1964 570 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 2758 2758 Size: 6097 SalI G'TCGA_C Cuts at: 1936 2712 3439 1936 Size: 776 727 4594 Fragments arranged by size: 4594 776 727 SanDI GG'GwC_CC Cuts at: 3197 3197 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 3374 4345 6057 3374 Size: 971 1712 3414 Fragments arranged by size: 3414 1712 971 SfcI C'TryA_G Cuts at: 826 1045 2661 2790 2875 3445 4580 5261 5452 Size: 219 1616 129 85 570 1135 681 191 Cuts at: 5452 826 Size: 1471 Fragments arranged by size: 1616 1471 1135 681 570 219 191 129 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 4201 5102 5364 820 Size: 197 926 279 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 2865 3888 493 Size: 2372 1023 2702 Fragments arranged by size: 2702 2372 1023 SpeI A'CTAG_T Cuts at: 152 2732 152 Size: 2580 3517 SphI G_CATG'C Cuts at: 1132 2435 3455 1132 Size: 1303 1020 3774 Fragments arranged by size: 3774 1303 1020 SspI AAT'ATT Cuts at: 5 52 2473 2703 2769 4021 5 Size: 47 2421 230 66 1252 2081 Fragments arranged by size: 2421 2081 1252 230 66 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 2955 3536 513 Size: 552 939 951 581 3074 Fragments arranged by size: 3074 951 939 581 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2431 2474 3180 4124 4141 4294 4479 Size: 1255 851 43 706 944 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 944 851 706 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 4343 6055 Size: 274 80 33 51 1443 1395 971 1712 Cuts at: 6055 96 Size: 138 Fragments arranged by size: 1712 1443 1395 971 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 2616 2849 4226 2616 Size: 233 1377 4487 Fragments arranged by size: 4487 1377 233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI