Restriction map for
pFN21AB5871
(Linear) (Six-base) MAPSORT of: KIBB5871.seq Check: 9391 from: 1 to: 1392
KIBB5871.orf
With 182 enzymes: SgfI *
March 22, 2012 15:32 ..
AccI GT'mk_AC
Cuts at: 0 104 708 862 1392
Size: 104 604 154 530
Fragments arranged by size:
604 530 154 104
Acc65I G'GTAC_C
Cuts at: 0 593 1392
Size: 593 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1392
Size: 919 473
AflII C'TTAA_G
Cuts at: 0 217 1392
Size: 217 1175
AlwNI CAG_nnn'CTG
Cuts at: 0 942 1392
Size: 942 450
ApoI r'AATT_y
Cuts at: 0 1069 1392
Size: 1069 323
AseI AT'TA_AT
Cuts at: 0 835 1392
Size: 835 557
AvaI C'yCGr_G
Cuts at: 0 371 1392
Size: 371 1021
BamHI G'GATC_C
Cuts at: 0 570 1392
Size: 570 822
BanI G'GyrC_C
Cuts at: 0 593 1392
Size: 593 799
BanII G_rGCy'C
Cuts at: 0 603 1392
Size: 603 789
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 1392
Size: 1016 376
BciVI GTATCCnnnnn_n'
Cuts at: 0 1330 1392
Size: 1330 62
BlpI GC'TnA_GC
Cuts at: 0 25 1392
Size: 25 1367
BmgBI CAC'GTC
Cuts at: 0 102 1392
Size: 102 1290
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 444 1392
Size: 179 265 948
Fragments arranged by size:
948 265 179
Bpu10I CC'TnA_GC
Cuts at: 0 491 1392
Size: 491 901
BsaAI yAC'GTr
Cuts at: 0 860 1392
Size: 860 532
BsaBI GATnn'nnATC
Cuts at: 0 569 1392
Size: 569 823
BseYI C'CCAG_C
Cuts at: 0 1352 1392
Size: 1352 40
BsmI GAATG_Cn'
Cuts at: 0 922 1392
Size: 922 470
Bsp1286I G_dGCh'C
Cuts at: 0 603 1392
Size: 603 789
BspHI T'CATG_A
Cuts at: 0 1250 1392
Size: 1250 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 431 468 1392
Size: 431 37 924
Fragments arranged by size:
924 431 37
BsrBI CCG'CTC
Cuts at: 0 36 42 1392
Size: 36 6 1350
Fragments arranged by size:
1350 36 6
BsrFI r'CCGG_y
Cuts at: 0 1039 1392
Size: 1039 353
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1147 1392
Size: 1147 245
BstYI r'GATC_y
Cuts at: 0 173 401 570 1392
Size: 173 228 169 822
Fragments arranged by size:
822 228 173 169
BstZ17I GTA'TAC
Cuts at: 0 863 1392
Size: 863 529
Bsu36I CC'TnA_GG
Cuts at: 0 1236 1392
Size: 1236 156
BtgI C'CryG_G
Cuts at: 0 950 1392
Size: 950 442
DraI TTT'AAA
Cuts at: 0 15 152 1392
Size: 15 137 1240
Fragments arranged by size:
1240 137 15
EaeI y'GGCC_r
Cuts at: 0 81 1155 1392
Size: 81 1074 237
Fragments arranged by size:
1074 237 81
EarI CTCTTCn'nnn_
Cuts at: 0 245 495 1392
Size: 245 250 897
Fragments arranged by size:
897 250 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1392
Size: 919 473
EcoNI CCTnn'n_nnAGG
Cuts at: 0 494 1392
Size: 494 898
EcoO109I rG'GnC_Cy
Cuts at: 0 51 487 993 1192 1392
Size: 51 436 506 199 200
Fragments arranged by size:
506 436 200 199 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1033 1392
Size: 1033 359
HincII GTy'rAC
Cuts at: 0 709 1392
Size: 709 683
KpnI G_GTAC'C
Cuts at: 0 597 1392
Size: 597 795
MscI TGG'CCA
Cuts at: 0 1157 1392
Size: 1157 235
MslI CAynn'nnrTG
Cuts at: 0 1145 1392
Size: 1145 247
MspA1I CmG'CkG
Cuts at: 0 753 1392
Size: 753 639
NcoI C'CATG_G
Cuts at: 0 950 1392
Size: 950 442
NspI r_CATG'y
Cuts at: 0 191 430 1285 1392
Size: 191 239 855 107
Fragments arranged by size:
855 239 191 107
PasI CC'CwG_GG
Cuts at: 0 1162 1392
Size: 1162 230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 293 325 1392
Size: 293 32 1067
Fragments arranged by size:
1067 293 32
PpuMI rG'GwC_Cy
Cuts at: 0 51 487 993 1192 1392
Size: 51 436 506 199 200
Fragments arranged by size:
506 436 200 199 51
PshAI GACnn'nnGTC
Cuts at: 0 1178 1392
Size: 1178 214
PstI C_TGCA'G
Cuts at: 0 789 874 1392
Size: 789 85 518
Fragments arranged by size:
789 518 85
PvuII CAG'CTG
Cuts at: 0 753 1392
Size: 753 639
SalI G'TCGA_C
Cuts at: 0 707 1392
Size: 707 685
SanDI GG'GwC_CC
Cuts at: 0 1192 1392
Size: 1192 200
ScaI AGT'ACT
Cuts at: 0 1369 1392
Size: 1369 23
SfcI C'TryA_G
Cuts at: 0 656 785 870 1392
Size: 656 129 85 522
Fragments arranged by size:
656 522 129 85
SmlI C'TyrA_G
Cuts at: 0 217 1392
Size: 217 1175
SnaBI TAC'GTA
Cuts at: 0 860 1392
Size: 860 532
SpeI A'CTAG_T
Cuts at: 0 727 1392
Size: 727 665
SphI G_CATG'C
Cuts at: 0 430 1392
Size: 430 962
SspI AAT'ATT
Cuts at: 0 468 698 764 1392
Size: 468 230 66 628
Fragments arranged by size:
628 468 230 66
StyI C'CwwG_G
Cuts at: 0 950 1392
Size: 950 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 469 1175 1392
Size: 426 43 706 217
Fragments arranged by size:
706 426 217 43
TatI w'GTAC_w
Cuts at: 0 1367 1392
Size: 1367 25
XmnI GAAnn'nnTTC
Cuts at: 0 611 844 1392
Size: 611 233 548
Fragments arranged by size:
611 548 233
Enzymes that do cut:
AccI Acc65I AcuI AflII AlwNI ApoI AseI AvaI
BamHI BanI BanII BbsI BciVI BlpI BmgBI BmrI
Bpu10I BsaAI BsaBI BseYI BsmI Bsp1286I BspHI BspMI
BsrBI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI DraI
EaeI EarI Eco57MI EcoNI EcoO109I FalI HincII KpnI
MscI MslI MspA1I NcoI NspI PasI PpiI PpuMI
PshAI PstI PvuII SalI SanDI ScaI SfcI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I
BmtI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SrfI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI