(Linear) (Six-base) MAPSORT of: KIEE3368.seq Check: 183 from: 1 to: 3264 KIEE3368.orf With 182 enzymes: SgfI * July 3, 2012 17:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 779 1571 3264 Size: 779 792 1693 Fragments arranged by size: 1693 792 779 Acc65I G'GTAC_C Cuts at: 0 562 3264 Size: 562 2702 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2581 2788 2901 2920 3158 3260 3264 Size: 2581 207 113 19 238 102 4 Fragments arranged by size: 2581 238 207 113 102 19 4 AlwNI CAG_nnn'CTG Cuts at: 0 54 318 1038 3264 Size: 54 264 720 2226 Fragments arranged by size: 2226 720 264 54 ApaI G_GGCC'C Cuts at: 0 580 671 1379 1642 3264 Size: 580 91 708 263 1622 Fragments arranged by size: 1622 708 580 263 91 ApoI r'AATT_y Cuts at: 0 3000 3115 3264 Size: 3000 115 149 Fragments arranged by size: 3000 149 115 AvaI C'yCGr_G Cuts at: 0 429 1651 2644 3264 Size: 429 1222 993 620 Fragments arranged by size: 1222 993 620 429 BanI G'GyrC_C Cuts at: 0 274 562 706 777 895 1161 1395 1492 Size: 274 288 144 71 118 266 234 97 Cuts at: 1492 1535 2029 2600 2742 3264 Size: 43 494 571 142 522 Fragments arranged by size: 571 522 494 288 274 266 234 144 142 118 97 71 43 BanII G_rGCy'C Cuts at: 0 360 485 580 671 932 997 1271 1379 Size: 360 125 95 91 261 65 274 108 Cuts at: 1379 1642 1652 2513 2824 3205 3264 Size: 263 10 861 311 381 59 Fragments arranged by size: 861 381 360 311 274 263 261 125 108 95 91 65 59 10 BbeI G_GCGC'C Cuts at: 0 899 1165 1496 3264 Size: 899 266 331 1768 Fragments arranged by size: 1768 899 331 266 BbsI GAAGACnn'nnnn_ Cuts at: 0 86 2095 2277 2569 3264 Size: 86 2009 182 292 695 Fragments arranged by size: 2009 695 292 182 86 BbvCI CC'TCA_GC Cuts at: 0 1348 3264 Size: 1348 1916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 46 80 366 400 520 554 1545 1579 Size: 46 34 286 34 120 34 991 34 Cuts at: 1579 3264 Size: 1685 Fragments arranged by size: 1685 991 286 120 46 34 34 34 34 BclI T'GATC_A Cuts at: 0 2774 3264 Size: 2774 490 BglI GCCn_nnn'nGGC Cuts at: 0 1123 3264 Size: 1123 2141 BglII A'GATC_T Cuts at: 0 2397 3264 Size: 2397 867 BlpI GC'TnA_GC Cuts at: 0 556 1916 2818 3264 Size: 556 1360 902 446 Fragments arranged by size: 1360 902 556 446 Bme1580I G_kGCm'C Cuts at: 0 277 580 671 732 1208 1379 1400 1540 Size: 277 303 91 61 476 171 21 140 Cuts at: 1540 1642 3264 Size: 102 1622 Fragments arranged by size: 1622 476 303 277 171 140 102 91 61 21 BmgBI CAC'GTC Cuts at: 0 1509 2136 3264 Size: 1509 627 1128 Fragments arranged by size: 1509 1128 627 BmrI ACTGGGnnnn_n' Cuts at: 0 490 1195 2450 2790 3264 Size: 490 705 1255 340 474 Fragments arranged by size: 1255 705 490 474 340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2722 3264 Size: 2722 542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 631 1177 1900 2919 3264 Size: 418 213 546 723 1019 345 Fragments arranged by size: 1019 723 546 418 345 213 Bpu10I CC'TnA_GC Cuts at: 0 1348 2291 3098 3264 Size: 1348 943 807 166 Fragments arranged by size: 1348 943 807 166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 249 346 3264 Size: 249 97 2918 Fragments arranged by size: 2918 249 97 BsaI GGTCTCn'nnnn_ Cuts at: 0 838 3264 Size: 838 2426 BsaAI yAC'GTr Cuts at: 0 633 936 1179 1827 3261 3264 Size: 633 303 243 648 1434 3 Fragments arranged by size: 1434 648 633 303 243 3 BsaHI Gr'CG_yC Cuts at: 0 835 896 1162 1454 1493 3264 Size: 835 61 266 292 39 1771 Fragments arranged by size: 1771 835 292 266 61 39 BsaWI w'CCGG_w Cuts at: 0 1529 1865 2117 2273 2945 3264 Size: 1529 336 252 156 672 319 Fragments arranged by size: 1529 672 336 319 252 156 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 628 1477 2644 3264 Size: 628 849 1167 620 Fragments arranged by size: 1167 849 628 620 BseYI C'CCAG_C Cuts at: 0 511 990 1207 1479 2512 2762 3264 Size: 511 479 217 272 1033 250 502 Fragments arranged by size: 1033 511 502 479 272 250 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 826 2421 3264 Size: 826 1595 843 Fragments arranged by size: 1595 843 826 BsiEI CG_ry'CG Cuts at: 0 628 766 1310 3264 Size: 628 138 544 1954 Fragments arranged by size: 1954 628 544 138 BsiHKAI G_wGCw'C Cuts at: 0 360 2297 2654 3104 3205 3264 Size: 360 1937 357 450 101 59 Fragments arranged by size: 1937 450 360 357 101 59 BsiWI C'GTAC_G Cuts at: 0 629 3264 Size: 629 2635 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1748 3264 Size: 1748 1516 Bsp1286I G_dGCh'C Cuts at: 0 277 360 485 580 671 732 932 997 Size: 277 83 125 95 91 61 200 65 Cuts at: 997 1208 1271 1379 1400 1540 1642 1652 2297 Size: 211 63 108 21 140 102 10 645 Cuts at: 2297 2513 2654 2824 3104 3205 3264 Size: 216 141 170 280 101 59 Fragments arranged by size: 645 280 277 216 211 200 170 141 140 125 108 102 101 95 91 83 65 63 61 59 21 10 BspEI T'CCGG_A Cuts at: 0 2273 2945 3264 Size: 2273 672 319 Fragments arranged by size: 2273 672 319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 779 1571 3264 Size: 779 792 1693 Fragments arranged by size: 1693 792 779 BsrBI CCG'CTC Cuts at: 0 560 3264 Size: 560 2704 BsrFI r'CCGG_y Cuts at: 0 44 299 526 898 961 1110 3264 Size: 44 255 227 372 63 149 2154 Fragments arranged by size: 2154 372 255 227 149 63 44 BssHII G'CGCG_C Cuts at: 0 749 1170 1172 3264 Size: 749 421 2 2092 Fragments arranged by size: 2092 749 421 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 54 309 447 1038 1320 1566 3264 Size: 54 255 138 591 282 246 1698 Fragments arranged by size: 1698 591 282 255 246 138 54 BstBI TT'CG_AA Cuts at: 0 518 3264 Size: 518 2746 BstXI CCAn_nnnn'nTGG Cuts at: 0 153 609 3264 Size: 153 456 2655 Fragments arranged by size: 2655 456 153 BstYI r'GATC_y Cuts at: 0 1937 2397 3264 Size: 1937 460 867 Fragments arranged by size: 1937 867 460 Bsu36I CC'TnA_GG Cuts at: 0 2095 2825 3264 Size: 2095 730 439 Fragments arranged by size: 2095 730 439 BtgI C'CryG_G Cuts at: 0 602 622 663 968 1240 2987 3264 Size: 602 20 41 305 272 1747 277 Fragments arranged by size: 1747 602 305 277 272 41 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2298 2622 3264 Size: 2298 324 642 Fragments arranged by size: 2298 642 324 BtsI GCAGTG_nn' Cuts at: 0 1111 3264 Size: 1111 2153 DraIII CAC_nnn'GTG Cuts at: 0 1329 3264 Size: 1329 1935 EaeI y'GGCC_r Cuts at: 0 351 625 672 763 1108 1307 1892 2159 Size: 351 274 47 91 345 199 585 267 Cuts at: 2159 2330 3264 Size: 171 934 Fragments arranged by size: 934 585 351 345 274 267 199 171 91 47 EagI C'GGCC_G Cuts at: 0 625 763 1307 3264 Size: 625 138 544 1957 Fragments arranged by size: 1957 625 544 138 EarI CTCTTCn'nnn_ Cuts at: 0 1327 1754 1759 2713 3264 Size: 1327 427 5 954 551 Fragments arranged by size: 1327 954 551 427 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1241 1535 1712 2895 3264 Size: 1241 294 177 1183 369 Fragments arranged by size: 1241 1183 369 294 177 EcoICRI GAG'CTC Cuts at: 0 358 3203 3264 Size: 358 2845 61 Fragments arranged by size: 2845 358 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 631 1177 1900 2581 2788 2901 2919 Size: 418 213 546 723 681 207 113 18 Cuts at: 2919 2920 3158 3260 3264 Size: 1 238 102 4 Fragments arranged by size: 723 681 546 418 238 213 207 113 102 18 4 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 2161 3126 3264 Size: 2161 965 138 Fragments arranged by size: 2161 965 138 EcoO109I rG'GnC_Cy Cuts at: 0 576 1375 1376 1639 2835 3264 Size: 576 799 1 263 1196 429 Fragments arranged by size: 1196 799 576 429 263 1 EcoRI G'AATT_C Cuts at: 0 3115 3264 Size: 3115 149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2065 2373 3264 Size: 2065 308 891 Fragments arranged by size: 2065 891 308 FspI TGC'GCA Cuts at: 0 1573 1611 3264 Size: 1573 38 1653 Fragments arranged by size: 1653 1573 38 HaeII r_GCGC'y Cuts at: 0 593 610 899 1165 1480 1496 1682 2924 Size: 593 17 289 266 315 16 186 1242 Cuts at: 2924 3264 Size: 340 Fragments arranged by size: 1242 593 340 315 289 266 186 17 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2518 2550 3264 Size: 2518 32 714 Fragments arranged by size: 2518 714 32 HincII GTy'rAC Cuts at: 0 1863 3264 Size: 1863 1401 HindIII A'AGCT_T Cuts at: 0 582 3185 3264 Size: 582 2603 79 Fragments arranged by size: 2603 582 79 HpaI GTT'AAC Cuts at: 0 1863 3264 Size: 1863 1401 KasI G'GCGC_C Cuts at: 0 895 1161 1492 3264 Size: 895 266 331 1772 Fragments arranged by size: 1772 895 331 266 KpnI G_GTAC'C Cuts at: 0 566 3264 Size: 566 2698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1562 2676 3264 Size: 1562 1114 588 Fragments arranged by size: 1562 1114 588 MscI TGG'CCA Cuts at: 0 1894 2332 3264 Size: 1894 438 932 Fragments arranged by size: 1894 932 438 MspA1I CmG'CkG Cuts at: 0 286 555 624 741 970 981 1242 1503 Size: 286 269 69 117 229 11 261 261 Cuts at: 1503 1878 2766 3264 Size: 375 888 498 Fragments arranged by size: 888 498 375 286 269 261 261 229 117 69 11 NaeI GCC'GGC Cuts at: 0 46 900 1112 3264 Size: 46 854 212 2152 Fragments arranged by size: 2152 854 212 46 NarI GG'CG_CC Cuts at: 0 896 1162 1493 3264 Size: 896 266 331 1771 Fragments arranged by size: 1771 896 331 266 NcoI C'CATG_G Cuts at: 0 2987 3264 Size: 2987 277 NgoMIV G'CCGG_C Cuts at: 0 44 898 1110 3264 Size: 44 854 212 2154 Fragments arranged by size: 2154 854 212 44 NotI GC'GGCC_GC Cuts at: 0 763 3264 Size: 763 2501 NspI r_CATG'y Cuts at: 0 988 1471 3264 Size: 988 483 1793 Fragments arranged by size: 1793 988 483 PasI CC'CwG_GG Cuts at: 0 792 2019 3264 Size: 792 1227 1245 Fragments arranged by size: 1245 1227 792 PflMI CCAn_nnn'nTGG Cuts at: 0 345 2352 2874 2988 3062 3264 Size: 345 2007 522 114 74 202 Fragments arranged by size: 2007 522 345 202 114 74 PmlI CAC'GTG Cuts at: 0 1179 1827 3264 Size: 1179 648 1437 Fragments arranged by size: 1437 1179 648 PpuMI rG'GwC_Cy Cuts at: 0 2835 3264 Size: 2835 429 PshAI GACnn'nnGTC Cuts at: 0 1300 3264 Size: 1300 1964 PspOMI G'GGCC_C Cuts at: 0 576 667 1375 1638 3264 Size: 576 91 708 263 1626 Fragments arranged by size: 1626 708 576 263 91 PstI C_TGCA'G Cuts at: 0 285 291 314 746 1145 1877 3264 Size: 285 6 23 432 399 732 1387 Fragments arranged by size: 1387 732 432 399 285 23 6 PvuII CAG'CTG Cuts at: 0 286 555 981 1878 2766 3264 Size: 286 269 426 897 888 498 Fragments arranged by size: 897 888 498 426 286 269 RsrII CG'GwC_CG Cuts at: 0 1249 3264 Size: 1249 2015 SacI G_AGCT'C Cuts at: 0 360 3205 3264 Size: 360 2845 59 Fragments arranged by size: 2845 360 59 SacII CC_GC'GG Cuts at: 0 625 971 1243 3264 Size: 625 346 272 2021 Fragments arranged by size: 2021 625 346 272 SapI GCTCTTCn'nnn_ Cuts at: 0 1327 3264 Size: 1327 1937 SfcI C'TryA_G Cuts at: 0 281 287 310 742 1141 1873 3264 Size: 281 6 23 432 399 732 1391 Fragments arranged by size: 1391 732 432 399 281 23 6 SfoI GGC'GCC Cuts at: 0 897 1163 1494 3264 Size: 897 266 331 1770 Fragments arranged by size: 1770 897 331 266 SmlI C'TyrA_G Cuts at: 0 228 361 3264 Size: 228 133 2903 Fragments arranged by size: 2903 228 133 SphI G_CATG'C Cuts at: 0 1471 3264 Size: 1471 1793 SspI AAT'ATT Cuts at: 0 2470 3264 Size: 2470 794 StuI AGG'CCT Cuts at: 0 3061 3264 Size: 3061 203 StyI C'CwwG_G Cuts at: 0 695 1457 1682 2987 3028 3056 3264 Size: 695 762 225 1305 41 28 208 Fragments arranged by size: 1305 762 695 225 208 41 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 704 708 959 1383 2169 3264 Size: 704 4 251 424 786 1095 Fragments arranged by size: 1095 786 704 424 251 4 TatI w'GTAC_w Cuts at: 0 401 2222 3264 Size: 401 1821 1042 Fragments arranged by size: 1821 1042 401 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1591 1623 3264 Size: 1591 32 1641 Fragments arranged by size: 1641 1591 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 346 3264 Size: 346 2918 Enzymes that do cut: AarI Acc65I AcuI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII SapI SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BmtI BsaBI BsaXI BsmI BspHI BsrDI BsrGI BssSI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRV FseI FspAI MfeI MluI MslI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI