Restriction map for
pFN21AE3060
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HaeIII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeIII
MluI MluNI MroI MscI MseI
MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 482 CGCG CG^CG
AciI 483 GCGG CCGC(-3/-1)
AcsI 64 GAATTT [GA]^AATT[CT]
AfaI 474 GTAC GT^AC
ApoI 64 GAATTT [GA]^AATT[CT]
BanI 508 GGTGCC G^G[CT][GA]CC
BcgI 381 CGAAAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 578 CCCGG CC^[GC]GG
BfaI 248 CTAG C^TAG
BlpI 73 GCTGAGC GC^T[ACGT]AGC
BpmI 58 CTCCAG CTGGAG(16/14)
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
BsaBI 294 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 540 CCTTGG C^C[ACGT][ACGT]GG
BseRI 337 GAGGAG GAGGAG(10/8)
BsrI 153 CCAGT ACTGG(1/-1)
BstF5I 105 GGATG GGATG(2/0)
BstUI 482 CGCG CG^CG
BstYI 420 AGATCC [GA]^GATC[CT]
Cac8I 186 GCTGGC GC[ACGT]^[ACGT]GC
CelII 73 GCTGAGC GC^T[ACGT]AGC
EarI 133 GAAGAG CTCTTC(1/4)
EcoT14I 540 CCTTGG C^C[AT][AT]GG
FauI 483 GCGGG CCCGC(4/6)
FokI 105 GGATG GGATG(9/13)
HapII 579 CCGG C^CGG
HgaI 480 GACGC GACGC(5/10)
HpaII 579 CCGG C^CGG
Ksp632I 133 GAAGAG CTCTTC(1/4)
MaeI 248 CTAG C^TAG
MaeII 559 ACGT A^CGT
MamI 294 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 200 CAATTG C^AATTG
MflI 420 AGATCC [GA]^GATC[CT]
MspI 579 CCGG C^CGG
MunI 200 CAATTG C^AATTG
MvnI 482 CGCG CG^CG
NciI 578 CCCGG CC^[GC]GG
NlaIV 508 GGTGCC GG[ACGT]^[ACGT]CC
NspI 232 GCATGT [GA]CATG^[CT]
PstI 181 CTGCAG CTGCA^G
RsaI 474 GTAC GT^AC
StyI 540 CCTTGG C^C[AT][AT]GG
TaqI 292 TCGA T^CGA
TthHB8I 292 TCGA T^CGA
XhoII 420 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 501 GTGCAC G^TGCAC
555 GTGCAC
AlwI 421 GATCC GGATC(4/5)
638 GGATC
ApaLI 501 GTGCAC G^TGCAC
555 GTGCAC
AspHI 501 GTGCAC G[AT]GC[AT]^C
555 GTGCAC
BsgI 392 CTGCAC GTGCAG(16/14)
276 GTGCAG
BsiHKAI 501 GTGCAC G[AT]GC[AT]^C
555 GTGCAC
BsiYI 477 CCTGACGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
579 CCGGAACTTGG
BslI 477 CCTGACGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
579 CCGGAACTTGG
BsmAI 22 GAGAC GTCTC(1/5)
446 GTCTC
BstNI 60 CCAGG CC^[AT]GG
97 CCAGG
DpnII 421 GATC ^GATC
639 GATC
EcoRII 60 CCAGG ^CC[AT]GG
97 CCAGG
MboI 421 GATC ^GATC
639 GATC
MspA1I 184 CAGCTG C[AC]G^C[GT]G
206 CAGCTG
MvaI 60 CCAGG CC^[AT]GG
97 CCAGG
NdeII 421 GATC ^GATC
639 GATC
PvuII 184 CAGCTG CAG^CTG
206 CAGCTG
Sau3AI 421 GATC ^GATC
639 GATC
SfcI 181 CTGCAG C^T[GA][CT]AG
209 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BbvI BmyI Bsp1286I BssKI
DdeI Fnu4HI HinfI ItaI MboII
MnlI MwoI NlaIII ScrFI TfiI
TseI Tsp509I