Restriction map for
pFN21AE5551
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MspA1I MvnI
MwoI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 417 GCGG CCGC(-3/-1)
AfaI 947 GTAC GT^AC
AflIII 1109 ACACGT A^C[GA][CT]GT
AlwI 484 GATCC GGATC(4/5)
Asp700I 959 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1018 GGTCC G^G[AT]CC
BanI 48 GGCACC G^G[CT][GA]CC
BbvI 91 GCAGC GCAGC(8/12)
BciVI 848 GGATAC GTATCC(6/5)
BclI 528 TGATCA T^GATCA
BcnI 647 CCGGG CC^[GC]GG
BglII 597 AGATCT A^GATCT
BmyI 662 GTGCCC G[AGT]GC[ACT]^C
BseRI 43 GAGGAG GAGGAG(10/8)
BsgI 1045 GTGCAG GTGCAG(16/14)
Bsp1286I 662 GTGCCC G[AGT]GC[ACT]^C
BspHI 518 TCATGA T^CATGA
BspMI 51 ACCTGC ACCTGC(4/8)
BstBI 1059 TTCGAA TT^CGAA
BstNI 96 CCTGG CC^[AT]GG
BstYI 597 AGATCT [GA]^GATC[CT]
Cfr13I 1018 GGTCC G^G[ACGT]CC
EarI 1056 CTCTTC CTCTTC(1/4)
EcoRII 96 CCTGG ^CC[AT]GG
EcoT14I 569 CCAAGG C^C[AT][AT]GG
FbaI 528 TGATCA T^GATCA
Fnu4HI 91 GCAGC GC^[ACGT]GC
HapII 647 CCGG C^CGG
HpaII 647 CCGG C^CGG
ItaI 91 GCAGC GC^[ACGT]GC
Ksp632I 1056 CTCTTC CTCTTC(1/4)
MflI 597 AGATCT [GA]^GATC[CT]
MspI 647 CCGG C^CGG
MvaI 96 CCTGG CC^[AT]GG
NciI 647 CCGGG CC^[GC]GG
NdeI 173 CATATG CA^TATG
NlaIV 48 GGCACC GG[ACGT]^[ACGT]CC
NspV 1059 TTCGAA TT^CGAA
PacI 1000 TTAATTAA TTAAT^TAA
PflMI 367 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 452 GAGTC GAGTC(4/5)
PshAI 1081 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 518 TCATGA T^CATGA
RsaI 947 GTAC GT^AC
Sau96I 1018 GGTCC G^G[ACGT]CC
ScaI 946 AGTACT AGT^ACT
SfaNI 154 GATGC GCATC(5/9)
SfcI 390 CTATAG C^T[GA][CT]AG
SfuI 1059 TTCGAA TT^CGAA
SmlI 593 CTCAAG C^T[CT][GA]AG
SspI 684 AATATT AAT^ATT
StyI 569 CCAAGG C^C[AT][AT]GG
TseI 91 GCAGC G^C[AT]GC
Tsp45I 102 GTCAC ^GT[GC]AC
Van91I 367 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 423 TCTAGA T^CTAGA
XcmI 1090 CCATTTTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 597 AGATCT [GA]^GATC[CT]
XmnI 959 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 454 GTCTAC GT^[AC][GT]AC
1139 GTCTAC
AluI 263 AGCT AG^CT
477 AGCT
AseI 999 ATTAAT AT^TAAT
1003 ATTAAT
AsnI 999 ATTAAT AT^TAAT
1003 ATTAAT
BfaI 322 CTAG C^TAG
424 CTAG
BsiYI 367 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCATAATAGGG
BslI 367 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCATAATAGGG
BsmFI 649 GGGAC GGGAC(10/14)
538 GTCCC
BsrI 827 ACTGG ACTGG(1/-1)
944 CCAGT
BssKI 96 CCTGG ^CC[ACGT]GG
647 CCGGG
BstF5I 309 GGATG GGATG(2/0)
587 GGATG
DdeI 488 CTAAG C^T[ACGT]AG
779 CTCAG
DraI 28 TTTAAA TTT^AAA
82 TTTAAA
FokI 309 GGATG GGATG(9/13)
587 GGATG
MaeI 322 CTAG C^TAG
424 CTAG
MaeIII 102 GTCAC ^GT[ACGT]AC
337 GTTAC
MfeI 401 CAATTG C^AATTG
1083 CAATTG
MslI 70 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
173 CATATGAATG
MunI 401 CAATTG C^AATTG
1083 CAATTG
PshBI 999 ATTAAT AT^TAAT
1003 ATTAAT
ScrFI 96 CCTGG CC^[ACGT]GG
647 CCGGG
TspRI 468 CAGTG CA[GC]TG[ACGT][ACGT]^
622 CACTG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI BsaJI DpnII HinfI
HphI MaeII MboI MboII MnlI
MseI NdeII NlaIII Sau3AI TaqI
TfiI Tru9I Tsp509I TthHB8I