(Circular) (Six-base) MAPSORT of: pFN21AE5551.seq Check: 881 from: 1 to: 5938 pFN21AE5551 5938 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2065 2065 Size: 5938 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2460 3145 3281 1937 Size: 523 685 136 4594 Fragments arranged by size: 4594 685 523 136 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4001 5385 5653 1632 Size: 2369 1384 268 1917 Fragments arranged by size: 2369 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 3114 4837 5814 1203 Size: 1911 1723 977 1327 Fragments arranged by size: 1911 1723 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 2079 1166 Size: 913 5025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 3681 5253 3681 Size: 1572 4366 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 2030 2084 2229 2759 3192 3206 3247 3473 Size: 537 54 145 530 433 14 41 226 Cuts at: 3473 3625 5734 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 537 530 433 226 152 145 54 41 14 AseI AT'TA_AT Cuts at: 160 1032 3005 3009 4492 160 Size: 872 1973 4 1483 1606 Fragments arranged by size: 1973 1606 1483 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 3263 1870 Size: 73 1320 4545 Fragments arranged by size: 4545 1320 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3259 4714 618 Size: 325 217 627 266 1206 1455 1842 Fragments arranged by size: 1842 1455 1206 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 3257 1391 Size: 1866 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2847 3830 5040 2847 Size: 983 1210 3745 Fragments arranged by size: 3745 1210 983 BclI T'GATC_A Cuts at: 1548 2533 1548 Size: 985 4953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2602 5933 2602 Size: 3331 2607 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2671 3999 5155 1790 Size: 881 1328 1156 2573 Fragments arranged by size: 2573 1328 1156 881 BmgBI CAC'GTC Cuts at: 1318 3117 1318 Size: 1799 4139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4598 1239 Size: 3359 2579 Bpu10I CC'TnA_GC Cuts at: 2493 2493 Size: 5938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 4063 4928 5226 2583 Size: 1480 865 298 3295 Fragments arranged by size: 3295 1480 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3745 4127 4762 275 Size: 53 83 186 936 2212 382 635 1451 Fragments arranged by size: 2212 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5713 Fragments arranged by size: 5713 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 1186 Size: 877 5061 BseYI C'CCAG_C Cuts at: 5141 5637 5141 Size: 496 5442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 1163 Size: 573 1335 4030 Fragments arranged by size: 4030 1335 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3999 4084 5155 3999 Size: 85 1071 4782 Fragments arranged by size: 4782 1071 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 3257 3999 4084 5155 1391 Size: 399 881 586 742 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 586 399 85 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 2523 3827 5557 2523 Size: 1304 1730 2904 Fragments arranged by size: 2904 1730 1304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 3293 844 Size: 1221 1228 3489 Fragments arranged by size: 3489 1228 1221 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 3065 4742 3065 Size: 1677 4261 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2602 3268 3635 4020 4037 5489 5575 5587 Size: 630 666 367 385 17 1452 86 12 Cuts at: 5587 5933 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 666 630 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2479 3454 4248 4268 2479 Size: 975 794 20 4149 Fragments arranged by size: 4149 975 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 2035 2089 3242 2035 Size: 54 1153 4731 Fragments arranged by size: 4731 1153 54 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3385 4274 5903 Size: 54 1425 50 275 18 1555 889 1629 Cuts at: 5903 8 Size: 43 Fragments arranged by size: 1629 1555 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3067 3868 1353 Size: 1714 801 3423 Fragments arranged by size: 3423 1714 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4001 4598 5385 5653 1239 Size: 393 2369 597 787 268 1524 Fragments arranged by size: 2369 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2406 2959 2406 Size: 553 5385 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2518 2550 2841 2873 4579 Size: 32 135 32 946 32 291 32 1706 Cuts at: 4579 4611 4653 4685 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1706 946 291 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 2406 3088 3537 2406 Size: 682 449 4807 Fragments arranged by size: 4807 682 449 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2779 5052 5236 1305 Size: 1474 2273 184 2007 Fragments arranged by size: 2273 2007 1474 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2182 3896 4255 4414 518 Size: 648 913 103 1714 359 159 2042 Fragments arranged by size: 2042 1714 913 648 359 159 103 MspA1I CmG'CkG Cuts at: 1606 3354 4031 5179 5424 1606 Size: 1748 677 1148 245 2120 Fragments arranged by size: 2120 1748 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 2179 387 Size: 1792 4146 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 3296 4841 1132 Size: 97 2067 1545 2229 Fragments arranged by size: 2229 2067 1545 97 PacI TTA_AT'TAA Cuts at: 3009 3009 Size: 5938 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2378 1108 Size: 1270 4668 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3090 1613 Size: 1477 4461 PsiI TTA'TAA Cuts at: 2864 3508 2864 Size: 644 5294 PstI C_TGCA'G Cuts at: 830 3290 830 Size: 2460 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 2953 4186 5898 2953 Size: 1233 1712 2993 Fragments arranged by size: 2993 1712 1233 SfcI C'TryA_G Cuts at: 826 1045 2395 3286 4421 5102 5293 826 Size: 219 1350 891 1135 681 191 1471 Fragments arranged by size: 1471 1350 1135 891 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 4042 4943 5205 820 Size: 197 926 655 1444 901 262 1553 Fragments arranged by size: 1553 1444 926 901 655 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 2691 3862 5 Size: 47 2639 1171 2081 Fragments arranged by size: 2639 2081 1171 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2574 3377 513 Size: 552 1509 803 3074 Fragments arranged by size: 3074 1509 803 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3965 3982 4135 4320 4765 325 Size: 1255 2385 17 153 185 445 1498 Fragments arranged by size: 2385 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2951 4184 5896 Size: 274 80 33 51 1443 974 1233 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1712 1443 1233 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5898 5930 5898 Size: 32 5906 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 2428 3274 2428 Size: 846 5092 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3102 3102 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2968 4067 2968 Size: 1099 4839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI