(Circular) (Six-base) MAPSORT of: pFN21AB5624.seq Check: 6951 from: 1 to: 11563 pFN21AB5624 11563 bp With 182 enzymes: SgfI * August 3, 2016 16:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6935 7059 6935 Size: 124 11439 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9373 278 Size: 53 83 186 936 7837 2468 Fragments arranged by size: 7837 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5156 5535 6186 8906 1937 Size: 3219 379 651 2720 4594 Fragments arranged by size: 4594 3219 2720 651 379 Acc65I G'GTAC_C Cuts at: 8884 8884 Size: 11563 AclI AA'CG_TT Cuts at: 1563 9690 10063 1563 Size: 8127 373 3063 Fragments arranged by size: 8127 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 2732 3854 6707 6803 7181 7363 7409 Size: 551 549 1122 2853 96 378 182 46 Cuts at: 7409 8690 9626 11010 11278 1632 Size: 1281 936 1384 268 1917 Fragments arranged by size: 2853 1917 1384 1281 1122 936 551 549 378 268 182 96 46 AfeI AGC'GCT Cuts at: 2234 4382 6498 2234 Size: 2148 2116 7299 Fragments arranged by size: 7299 2148 2116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11366 AflIII A'CryG_T Cuts at: 1203 2559 3052 3333 3598 5482 6219 8325 10462 Size: 1356 493 281 265 1884 737 2106 2137 Cuts at: 10462 11439 1203 Size: 977 1327 Fragments arranged by size: 2137 2106 1884 1356 1327 977 737 493 281 265 AgeI A'CCGG_T Cuts at: 10394 10394 Size: 11563 AhdI GACnn_n'nnGTC Cuts at: 10292 10292 Size: 11563 AleI CACnn'nnGTG Cuts at: 1166 4217 8075 1166 Size: 3051 3858 4654 Fragments arranged by size: 4654 3858 3051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3948 6083 6587 7993 1184 Size: 2764 2135 504 1406 4754 Fragments arranged by size: 4754 2764 2135 1406 504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8417 8449 8417 Size: 32 11531 AlwNI CAG_nnn'CTG Cuts at: 3367 3392 4741 5681 5887 6680 7204 8659 9306 Size: 25 1349 940 206 793 524 1455 647 Cuts at: 9306 10878 3367 Size: 1572 4052 Fragments arranged by size: 4052 1572 1455 1349 940 793 647 524 206 25 ApaI G_GGCC'C Cuts at: 4812 6316 6632 6750 4812 Size: 1504 316 118 9625 Fragments arranged by size: 9625 1504 316 118 ApaLI G'TGCA_C Cuts at: 3448 6320 9620 10776 3448 Size: 2872 3300 1156 4235 Fragments arranged by size: 4235 3300 2872 1156 ApoI r'AATT_y Cuts at: 1493 4627 6801 6818 7739 8872 9098 9250 11359 Size: 3134 2174 17 921 1133 226 152 2109 Cuts at: 11359 1493 Size: 1697 Fragments arranged by size: 3134 2174 2109 1697 1133 921 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 10117 160 Size: 872 9085 1606 Fragments arranged by size: 9085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11563 AvaI C'yCGr_G Cuts at: 1870 1943 3126 3644 4116 5255 5915 8888 1870 Size: 73 1183 518 472 1139 660 2973 4545 Fragments arranged by size: 4545 2973 1183 1139 660 518 472 73 BamHI G'GATC_C Cuts at: 4059 7383 7756 8893 9260 4059 Size: 3324 373 1137 367 6362 Fragments arranged by size: 6362 3324 1137 373 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 3199 4685 6363 6584 Size: 325 217 627 288 1124 1486 1678 221 Cuts at: 6584 6829 8578 8884 10339 618 Size: 245 1749 306 1455 1842 Fragments arranged by size: 1842 1749 1678 1486 1455 1124 627 325 306 288 245 221 217 BanII G_rGCy'C Cuts at: 1391 3471 3494 4317 4477 4812 4908 5100 5264 Size: 2080 23 823 160 335 96 192 164 Cuts at: 5264 5313 5354 5562 6316 6632 6639 6750 6892 Size: 49 41 208 754 316 7 111 142 Cuts at: 6892 8134 8181 8253 8882 1391 Size: 1242 47 72 629 4072 Fragments arranged by size: 4072 2080 1242 823 754 629 335 316 208 192 164 160 142 111 96 72 49 47 41 23 7 BbeI G_GCGC'C Cuts at: 2079 3203 4689 6588 8582 2079 Size: 1124 1486 1899 1994 5060 Fragments arranged by size: 5060 1994 1899 1486 1124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2186 2998 3737 4627 4893 5616 6695 Size: 971 287 812 739 890 266 723 1079 Cuts at: 6695 8746 928 Size: 2051 3745 Fragments arranged by size: 3745 2051 1079 971 890 812 739 723 287 266 BbvCI CC'TCA_GC Cuts at: 4371 8048 4371 Size: 3677 7886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2827 2861 8427 8461 8576 8610 9754 Size: 34 890 34 5566 34 115 34 1144 Cuts at: 9754 9788 1903 Size: 34 3678 Fragments arranged by size: 5566 3678 1144 890 115 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6464 9455 10665 6464 Size: 2991 1210 7362 Fragments arranged by size: 7362 2991 1210 BclI T'GATC_A Cuts at: 1548 7311 8145 1548 Size: 5763 834 4966 Fragments arranged by size: 5763 4966 834 BfrBI ATG'CAT Cuts at: 2203 2203 Size: 11563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3290 5030 6030 Size: 107 122 71 631 605 1618 1740 1000 Cuts at: 6030 8120 8557 10174 136 Size: 2090 437 1617 1525 Fragments arranged by size: 2090 1740 1618 1617 1525 1000 631 605 437 122 107 71 BglII A'GATC_T Cuts at: 2511 4953 11558 2511 Size: 2442 6605 2516 Fragments arranged by size: 6605 2516 2442 BlpI GC'TnA_GC Cuts at: 4746 4902 6083 6252 8980 4746 Size: 156 1181 169 2728 7329 Fragments arranged by size: 7329 2728 1181 169 156 Bme1580I G_kGCm'C Cuts at: 1790 3452 4812 5754 6316 6324 6632 6750 7339 Size: 1662 1360 942 562 8 308 118 589 Cuts at: 7339 9624 10780 1790 Size: 2285 1156 2573 Fragments arranged by size: 2573 2285 1662 1360 1156 942 589 562 308 118 8 BmgBI CAC'GTC Cuts at: 1318 3055 3883 5050 7282 1318 Size: 1737 828 1167 2232 5599 Fragments arranged by size: 5599 2232 1737 1167 828 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3199 5468 6421 Size: 416 53 83 399 24 1782 2269 953 Cuts at: 6421 7420 8425 9294 10252 442 Size: 999 1005 869 958 1753 Fragments arranged by size: 2269 1782 1753 1005 999 958 953 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11563 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2641 4771 5119 6433 7012 2641 Size: 2130 348 1314 579 7192 Fragments arranged by size: 7192 2130 1314 579 348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4002 4094 5540 5591 5909 6428 6638 7019 Size: 2763 92 1446 51 318 519 210 381 Cuts at: 7019 7208 7322 8046 8330 8837 8846 10223 1239 Size: 189 114 724 284 507 9 1377 2579 Fragments arranged by size: 2763 2579 1446 1377 724 519 507 381 318 284 210 189 114 92 51 9 Bpu10I CC'TnA_GC Cuts at: 2236 4371 5909 6633 8048 2236 Size: 2135 1538 724 1415 5751 Fragments arranged by size: 5751 2135 1538 1415 724 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2084 2837 3260 3770 4892 4970 5330 6419 9688 Size: 753 423 510 1122 78 360 1089 3269 Cuts at: 9688 10553 10851 2084 Size: 865 298 2796 Fragments arranged by size: 3269 2796 1122 1089 865 753 510 423 360 298 78 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3056 3608 5193 6011 6380 7691 8379 Size: 617 1557 552 1585 818 369 1311 688 Cuts at: 8379 8433 8684 8813 10226 882 Size: 54 251 129 1413 2219 Fragments arranged by size: 2219 1585 1557 1413 1311 818 688 617 552 369 251 129 54 BsaAI yAC'GTr Cuts at: 493 1204 2722 3375 3601 3634 4267 5483 6106 Size: 711 1518 653 226 33 633 1216 623 Cuts at: 6106 7663 8326 8458 9354 493 Size: 1557 663 132 896 2702 Fragments arranged by size: 2702 1557 1518 1216 896 711 663 653 633 623 226 132 33 BsaBI GATnn'nnATC Cuts at: 4208 4952 6512 11557 4208 Size: 744 1560 5045 4214 Fragments arranged by size: 5045 4214 1560 744 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 3200 4686 6585 Size: 53 83 186 936 543 1124 1486 1899 Cuts at: 6585 8579 9370 9752 10387 275 Size: 1994 791 382 635 1451 Fragments arranged by size: 1994 1899 1486 1451 1124 936 791 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2764 5334 5820 8778 9996 10394 10668 10815 11226 Size: 2570 486 2958 1218 398 274 147 411 Cuts at: 11226 2764 Size: 3101 Fragments arranged by size: 3101 2958 2570 1218 486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3320 3350 4388 4418 5138 Size: 30 165 30 1917 30 1038 30 720 Cuts at: 5138 5168 5228 5258 8417 8447 1178 Size: 30 60 30 3159 30 4294 Fragments arranged by size: 4294 3159 1917 1038 720 165 60 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 3773 5738 6146 6602 6749 7886 7904 Size: 843 1744 1965 408 456 147 1137 18 Cuts at: 7904 7907 8095 8098 8438 8581 1186 Size: 3 188 3 340 143 4168 Fragments arranged by size: 4168 1965 1744 1137 843 456 408 340 188 147 143 18 3 3 BseYI C'CCAG_C Cuts at: 3845 5413 5671 5947 6375 6575 6774 7407 7734 Size: 1568 258 276 428 200 199 633 327 Cuts at: 7734 8790 8854 10766 11262 3845 Size: 1056 64 1912 496 4146 Fragments arranged by size: 4146 1912 1568 1056 633 496 428 327 276 258 200 199 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2265 3545 5489 6026 7499 1163 Size: 573 529 1280 1944 537 1473 5227 Fragments arranged by size: 5227 1944 1473 1280 573 537 529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3285 4350 7484 8127 9013 9774 Size: 275 186 1284 1065 3134 643 886 761 Cuts at: 9774 10802 1540 Size: 1028 2301 Fragments arranged by size: 3134 2301 1284 1065 1028 886 761 643 275 186 BsiHKAI G_wGCw'C Cuts at: 3452 4908 5100 5354 5562 5632 5910 6324 6405 Size: 1456 192 254 208 70 278 414 81 Cuts at: 6405 6606 6639 7370 8062 8072 8181 8253 9624 Size: 201 33 731 692 10 109 72 1371 Cuts at: 9624 9709 10780 3452 Size: 85 1071 4235 Fragments arranged by size: 4235 1456 1371 1071 731 692 414 278 254 208 201 192 109 85 81 72 70 33 10 BsmI GAATG_Cn' Cuts at: 9074 9167 9074 Size: 93 11470 BsmBI CGTCTCn'nnnn_ Cuts at: 2828 4235 2828 Size: 1407 10156 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3452 3471 3494 4317 4477 4812 4908 Size: 399 1662 19 23 823 160 335 96 Cuts at: 4908 5100 5264 5313 5354 5562 5632 5754 5910 Size: 192 164 49 41 208 70 122 156 Cuts at: 5910 6316 6324 6405 6606 6632 6639 6750 6892 Size: 406 8 81 201 26 7 111 142 Cuts at: 6892 7339 7370 8062 8072 8134 8181 8253 8882 Size: 447 31 692 10 62 47 72 629 Cuts at: 8882 9624 9709 10780 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1662 1071 823 742 692 629 447 406 399 335 208 201 192 164 160 156 142 122 111 96 85 81 72 70 62 49 47 41 31 26 23 19 10 8 7 BspEI T'CCGG_A Cuts at: 5334 8778 11226 5334 Size: 3444 2448 5671 Fragments arranged by size: 5671 3444 2448 BspHI T'CATG_A Cuts at: 4126 7501 7995 9452 11182 4126 Size: 3375 494 1457 1730 4507 Fragments arranged by size: 4507 3375 1730 1457 494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5452 6935 6945 7059 8397 8918 844 Size: 4608 1483 10 114 1338 521 3489 Fragments arranged by size: 4608 3489 1483 1338 521 114 10 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2158 4776 5410 8451 9450 1394 Size: 429 196 139 2618 634 3041 999 3507 Fragments arranged by size: 3507 3041 2618 999 634 429 196 139 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 8784 10058 10232 65 Size: 1183 1071 6465 1274 174 1396 Fragments arranged by size: 6465 1396 1274 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3678 6029 10207 10394 1700 Size: 110 1868 2351 4178 187 2869 Fragments arranged by size: 4178 2869 2351 1868 187 110 BsrGI T'GTAC_A Cuts at: 96 3411 5055 7625 96 Size: 3315 1644 2570 4034 Fragments arranged by size: 4034 3315 2570 1644 BssHII G'CGCG_C Cuts at: 1925 2032 1925 Size: 107 11456 BssSI C'ACGA_G Cuts at: 9623 10635 9623 Size: 1012 10551 BstAPI GCAn_nnn'nTGC Cuts at: 794 4509 6004 7960 794 Size: 3715 1495 1956 4397 Fragments arranged by size: 4397 3715 1956 1495 BstBI TT'CG_AA Cuts at: 6663 10367 6663 Size: 3704 7859 BstEII G'GTnAC_C Cuts at: 1184 3082 1184 Size: 1898 9665 BstXI CCAn_nnnn'nTGG Cuts at: 3079 4495 5947 3079 Size: 1416 1452 8695 Fragments arranged by size: 8695 1452 1416 BstYI r'GATC_y Cuts at: 1972 2416 2488 2511 2715 4059 4203 4953 6507 Size: 444 72 23 204 1344 144 750 1554 Cuts at: 6507 7383 7756 8893 9260 9645 9662 11114 11200 Size: 876 373 1137 367 385 17 1452 86 Cuts at: 11200 11212 11558 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1554 1452 1344 1137 876 750 444 385 373 367 346 204 144 86 72 23 17 12 Bsu36I CC'TnA_GG Cuts at: 3227 8466 3227 Size: 5239 6324 BtgI C'CryG_G Cuts at: 513 1065 3139 3312 3348 3462 3936 4350 4488 Size: 552 2074 173 36 114 474 414 138 Cuts at: 4488 4930 5313 5940 5993 6883 8677 513 Size: 442 383 627 53 890 1794 3399 Fragments arranged by size: 3399 2074 1794 890 627 552 474 442 414 383 173 138 114 53 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6742 7546 7639 487 Size: 683 56 5516 804 93 4411 Fragments arranged by size: 5516 4411 804 683 93 56 BtsI GCAGTG_nn' Cuts at: 2248 2253 4019 6067 6070 8029 8062 9079 9873 Size: 5 1766 2048 3 1959 33 1017 794 Cuts at: 9873 9893 2248 Size: 20 3918 Fragments arranged by size: 3918 2048 1959 1766 1017 794 33 20 5 3 ClaI AT'CG_AT Cuts at: 1554 2904 1554 Size: 1350 10213 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11528 DraI TTT'AAA Cuts at: 8867 8867 Size: 11563 DraIII CAC_nnn'GTG Cuts at: 2440 2482 3504 4324 7179 2440 Size: 42 1022 820 2855 6824 Fragments arranged by size: 6824 2855 1022 820 42 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5207 10570 809 Size: 507 3891 5363 1802 Fragments arranged by size: 5363 3891 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3110 3282 Size: 54 1425 50 275 18 1078 202 172 Cuts at: 3282 3345 3369 4347 5184 5595 6792 7206 7341 Size: 63 24 978 837 411 1197 414 135 Cuts at: 7341 8019 9010 9899 11528 8 Size: 678 991 889 1629 43 Fragments arranged by size: 1629 1425 1197 1078 991 978 889 837 678 414 411 275 202 172 135 63 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3282 4347 9010 1537 Size: 275 1470 1065 4663 4090 Fragments arranged by size: 4663 4090 1470 1065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 3233 3750 3789 4157 4218 4859 4998 Size: 1257 623 517 39 368 61 641 139 Cuts at: 4998 5359 5567 5687 5870 6506 6779 7022 7385 Size: 361 208 120 183 636 273 243 363 Cuts at: 7385 7553 7893 8527 9493 1353 Size: 168 340 634 966 3423 Fragments arranged by size: 3423 1257 966 641 636 634 623 517 368 363 361 340 273 243 208 183 168 139 120 61 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2684 4190 6446 7788 7919 10150 Size: 1064 138 1095 1506 2256 1342 131 2231 Cuts at: 10150 10524 10670 387 Size: 374 146 1280 Fragments arranged by size: 2256 2231 1506 1342 1280 1095 1064 374 146 138 131 EcoICRI GAG'CTC Cuts at: 4906 5098 5352 5560 6637 8179 8251 4906 Size: 192 254 208 1077 1542 72 8218 Fragments arranged by size: 8218 1542 1077 254 208 192 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2732 3854 4002 4094 5540 5591 Size: 393 551 549 1122 148 92 1446 51 Cuts at: 5591 5909 6428 6638 6707 6803 7019 7181 7208 Size: 318 519 210 69 96 216 162 27 Cuts at: 7208 7322 7363 7409 8046 8330 8690 8837 8846 Size: 114 41 46 637 284 360 147 9 Cuts at: 8846 9626 10223 11010 11278 1239 Size: 780 597 787 268 1524 Fragments arranged by size: 1524 1446 1122 787 780 637 597 551 549 519 393 360 318 284 268 216 210 162 148 147 114 96 92 69 51 46 41 27 9 EcoNI CCTnn'n_nnAGG Cuts at: 5459 6620 6935 6986 5459 Size: 1161 315 51 10036 Fragments arranged by size: 10036 1161 315 51 EcoO109I rG'GnC_Cy Cuts at: 2993 3640 4569 4809 5734 6163 6313 6629 6746 Size: 647 929 240 925 429 150 316 117 Cuts at: 6746 6865 8656 2993 Size: 119 1791 5900 Fragments arranged by size: 5900 1791 929 925 647 429 316 240 150 119 117 EcoRI G'AATT_C Cuts at: 6818 8872 9250 6818 Size: 2054 378 9131 Fragments arranged by size: 9131 2054 378 EcoRV GAT'ATC Cuts at: 3670 3670 Size: 11563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4732 7564 7717 4732 Size: 2832 153 8578 Fragments arranged by size: 8578 2832 153 FseI GG_CCGG'CC Cuts at: 6033 6033 Size: 11563 FspI TGC'GCA Cuts at: 4466 6560 10069 4466 Size: 2094 3509 5960 Fragments arranged by size: 5960 3509 2094 HaeII r_GCGC'y Cuts at: 1678 2079 2107 2236 3203 4384 4689 5182 5424 Size: 401 28 129 967 1181 305 493 242 Cuts at: 5424 5863 6053 6266 6500 6588 6742 6883 7042 Size: 439 190 213 234 88 154 141 159 Cuts at: 7042 7579 7639 8582 10710 1678 Size: 537 60 943 2128 2531 Fragments arranged by size: 2531 2128 1181 967 943 537 493 439 401 305 242 234 213 190 159 154 141 129 88 60 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5138 5170 5869 5901 6379 Size: 32 135 32 3566 32 699 32 478 Cuts at: 6379 6403 6411 6435 6705 6737 10204 10236 10278 Size: 24 8 24 270 32 3467 32 42 Cuts at: 10278 10310 1373 Size: 32 2626 Fragments arranged by size: 3566 3467 2626 699 478 270 135 42 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 7213 7240 8907 9153 669 Size: 988 281 5275 27 1667 246 3079 Fragments arranged by size: 5275 3079 1667 988 281 246 27 HindIII A'AGCT_T Cuts at: 748 3821 748 Size: 3073 8490 HpaI GTT'AAC Cuts at: 9153 9153 Size: 11563 KasI G'GCGC_C Cuts at: 2075 3199 4685 6584 8578 2075 Size: 1124 1486 1899 1994 5060 Fragments arranged by size: 5060 1994 1899 1486 1124 KpnI G_GTAC'C Cuts at: 8888 8888 Size: 11563 MfeI C'AATT_G Cuts at: 9162 9162 Size: 11563 MluI A'CGCG_T Cuts at: 2559 11439 2559 Size: 8880 2683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2628 6201 7035 7938 8107 8592 10677 10861 Size: 1323 3573 834 903 169 485 2085 184 Cuts at: 10861 1305 Size: 2007 Fragments arranged by size: 3573 2085 2007 1323 903 834 485 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 2910 3112 3347 3371 6794 Size: 54 1425 343 1078 202 235 24 3423 Cuts at: 6794 10 Size: 4779 Fragments arranged by size: 4779 3423 1425 1078 343 235 202 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2198 3077 3215 4217 4397 4493 7400 Size: 648 1032 879 138 1002 180 96 2907 Cuts at: 7400 8075 8603 8702 9521 9880 10039 518 Size: 675 528 99 819 359 159 2042 Fragments arranged by size: 2907 2042 1032 1002 879 819 675 648 528 359 180 159 138 99 96 MspA1I CmG'CkG Cuts at: 1606 3721 3997 4072 4249 4420 4932 4945 5845 Size: 2115 276 75 177 171 512 13 900 Cuts at: 5845 6355 6774 7473 8484 8679 8715 8854 8979 Size: 510 419 699 1011 195 36 139 125 Cuts at: 8979 9656 10804 11049 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 2115 1148 1011 900 699 677 512 510 419 276 245 195 177 171 139 125 75 36 13 NaeI GCC'GGC Cuts at: 6031 6031 Size: 11563 NarI GG'CG_CC Cuts at: 2076 3200 4686 6585 8579 2076 Size: 1124 1486 1899 1994 5060 Fragments arranged by size: 5060 1994 1899 1486 1124 NcoI C'CATG_G Cuts at: 513 1065 3462 3936 4488 6883 513 Size: 552 2397 474 552 2395 5193 Fragments arranged by size: 5193 2397 2395 552 552 474 NdeI CA'TA_TG Cuts at: 387 387 Size: 11563 NgoMIV G'CCGG_C Cuts at: 6029 6029 Size: 11563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11563 NotI GC'GGCC_GC Cuts at: 3282 9010 3282 Size: 5728 5835 NsiI A_TGCA'T Cuts at: 2205 2205 Size: 11563 NspI r_CATG'y Cuts at: 1132 1229 2203 2668 3154 3337 5077 6223 8701 Size: 97 974 465 486 183 1740 1146 2478 Cuts at: 8701 8806 8921 10466 1132 Size: 105 115 1545 2229 Fragments arranged by size: 2478 2229 1740 1545 1146 974 486 465 183 115 105 97 PasI CC'CwG_GG Cuts at: 1348 5785 1348 Size: 4437 7126 PciI A'CATG_T Cuts at: 3333 6219 10462 3333 Size: 2886 4243 4434 Fragments arranged by size: 4434 4243 2886 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 3367 4741 1108 Size: 1374 885 1374 7930 Fragments arranged by size: 7930 1374 1374 885 PfoI T'CCnGG_A Cuts at: 3057 4287 5925 5982 6615 6867 8137 8814 3057 Size: 1230 1638 57 633 252 1270 677 5806 Fragments arranged by size: 5806 1638 1270 1230 677 633 252 57 PmeI GTTT'AAAC Cuts at: 8867 8867 Size: 11563 PmlI CAC'GTG Cuts at: 2722 3601 3634 5483 2722 Size: 879 33 1849 8802 Fragments arranged by size: 8802 1849 879 33 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 8417 8449 9632 9664 11145 11177 3557 Size: 32 4828 32 1183 32 1481 32 3943 Fragments arranged by size: 4828 3943 1481 1183 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5734 6163 6865 8656 5734 Size: 429 702 1791 8641 Fragments arranged by size: 8641 1791 702 429 PshAI GACnn'nnGTC Cuts at: 1613 3620 7110 1613 Size: 2007 3490 6066 Fragments arranged by size: 6066 3490 2007 PsiI TTA'TAA Cuts at: 9133 9133 Size: 11563 PspOMI G'GGCC_C Cuts at: 4808 6312 6628 6746 4808 Size: 1504 316 118 9625 Fragments arranged by size: 9625 1504 316 118 PspXI vC'TCGA_Gb Cuts at: 3644 3644 Size: 11563 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4862 4894 4862 Size: 32 11531 PstI C_TGCA'G Cuts at: 830 2592 3720 3927 4419 4944 5460 5850 6701 Size: 1762 1128 207 492 525 516 390 851 Cuts at: 6701 7557 8655 8714 8841 8915 830 Size: 856 1098 59 127 74 3478 Fragments arranged by size: 3478 1762 1128 1098 856 851 525 516 492 390 207 127 74 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11563 PvuII CAG'CTG Cuts at: 4072 4420 4945 5845 6355 6774 8484 8715 8854 Size: 348 525 900 510 419 1710 231 139 Cuts at: 8854 4072 Size: 6781 Fragments arranged by size: 6781 1710 900 525 510 419 348 231 139 SacI G_AGCT'C Cuts at: 4908 5100 5354 5562 6639 8181 8253 4908 Size: 192 254 208 1077 1542 72 8218 Fragments arranged by size: 8218 1542 1077 254 208 192 72 SacII CC_GC'GG Cuts at: 4933 8680 4933 Size: 3747 7816 SalI G'TCGA_C Cuts at: 1936 8905 1936 Size: 6969 4594 SapI GCTCTTCn'nnn_ Cuts at: 4218 5359 5567 6506 7385 7553 4218 Size: 1141 208 939 879 168 8228 Fragments arranged by size: 8228 1141 939 879 208 168 SbfI CC_TGCA'GG Cuts at: 8655 8915 8655 Size: 260 11303 ScaI AGT'ACT Cuts at: 9811 11523 9811 Size: 1712 9851 SexAI A'CCwGG_T Cuts at: 7452 8364 8658 7452 Size: 912 294 10357 Fragments arranged by size: 10357 912 294 SfcI C'TryA_G Cuts at: 826 1045 2588 3716 3923 4415 4940 5019 5456 Size: 219 1543 1128 207 492 525 79 437 Cuts at: 5456 5846 6697 7068 7553 8624 8651 8710 8837 Size: 390 851 371 485 1071 27 59 127 Cuts at: 8837 8911 10046 10727 10918 826 Size: 74 1135 681 191 1471 Fragments arranged by size: 1543 1471 1135 1128 1071 851 681 525 492 485 437 390 371 219 207 191 127 79 74 59 27 SfiI GGCCn_nnn'nGGCC Cuts at: 3290 3290 Size: 11563 SfoI GGC'GCC Cuts at: 2077 3201 4687 6586 8580 2077 Size: 1124 1486 1899 1994 5060 Fragments arranged by size: 5060 1994 1899 1486 1124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11563 SmaI CCC'GGG Cuts at: 1872 3128 5917 8890 1872 Size: 1256 2789 2973 4545 Fragments arranged by size: 4545 2973 2789 1256 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 2852 3275 3644 3749 4907 Size: 197 926 156 753 423 369 105 1158 Cuts at: 4907 4985 5345 6398 9667 10568 10830 820 Size: 78 360 1053 3269 901 262 1553 Fragments arranged by size: 3269 1553 1158 1053 926 901 753 423 369 360 262 197 156 105 78 SnaBI TAC'GTA Cuts at: 493 9354 493 Size: 8861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11563 SphI G_CATG'C Cuts at: 1132 3154 8701 8921 1132 Size: 2022 5547 220 3774 Fragments arranged by size: 5547 3774 2022 220 SrfI GCCC'GGGC Cuts at: 3128 3128 Size: 11563 SspI AAT'ATT Cuts at: 5 52 8775 9487 5 Size: 47 8723 712 2081 Fragments arranged by size: 8723 2081 712 47 StuI AGG'CCT Cuts at: 1517 3512 3530 4979 5603 6941 7295 1517 Size: 1995 18 1449 624 1338 354 5785 Fragments arranged by size: 5785 1995 1449 1338 624 354 18 StyI C'CwwG_G Cuts at: 513 1065 3288 3462 3936 4215 4488 4839 5025 Size: 552 2223 174 474 279 273 351 186 Cuts at: 5025 6580 6883 9002 513 Size: 1555 303 2119 3074 Fragments arranged by size: 3074 2223 2119 1555 552 474 351 303 279 273 186 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3223 3444 4550 5685 7076 7399 7439 Size: 1255 1643 221 1106 1135 1391 323 40 Cuts at: 7439 7470 8762 9590 9607 9760 9945 10390 325 Size: 31 1292 828 17 153 185 445 1498 Fragments arranged by size: 1643 1498 1391 1292 1255 1135 1106 828 445 323 221 185 153 40 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3192 3411 5055 Size: 274 80 33 51 1443 1215 219 1644 Cuts at: 5055 6591 7625 7668 8706 9809 11521 96 Size: 1536 1034 43 1038 1103 1712 138 Fragments arranged by size: 1712 1644 1536 1443 1215 1103 1038 1034 274 219 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3320 3352 3442 3474 5978 6010 11523 11555 3320 Size: 32 90 32 2504 32 5513 32 3328 Fragments arranged by size: 5513 3328 2504 90 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 8660 1316 Size: 7344 4219 XbaI T'CTAG_A Cuts at: 8899 8899 Size: 11563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 3460 7180 7660 2416 Size: 1044 3720 480 6319 Fragments arranged by size: 6319 3720 1044 480 XhoI C'TCGA_G Cuts at: 1943 3644 1943 Size: 1701 9862 XmaI C'CCGG_G Cuts at: 1870 3126 5915 8888 1870 Size: 1256 2789 2973 4545 Fragments arranged by size: 4545 2973 2789 1256 XmnI GAAnn'nnTTC Cuts at: 4445 7547 7743 7789 7988 9692 4445 Size: 3102 196 46 199 1704 6316 Fragments arranged by size: 6316 3102 1704 199 196 46 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9371 276 Size: 53 83 186 936 7837 2468 Fragments arranged by size: 7837 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BsiWI BstZ17I FspAI NruI PacI RsrII SanDI SwaI