(Linear) (Six-base) MAPSORT of: KIBB5624.seq Check: 2568 from: 1 to: 6858 KIBB5624.orf With 182 enzymes: SgfI * August 3, 2016 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4930 5054 6858 Size: 4930 124 1804 Fragments arranged by size: 4930 1804 124 AccI GT'mk_AC Cuts at: 0 3151 3530 4181 6858 Size: 3151 379 651 2677 Fragments arranged by size: 3151 2677 651 379 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 727 1849 4702 4798 5176 5358 5404 Size: 178 549 1122 2853 96 378 182 46 Cuts at: 5404 6685 6858 Size: 1281 173 Fragments arranged by size: 2853 1281 1122 549 378 182 178 173 96 46 AfeI AGC'GCT Cuts at: 0 229 2377 4493 6858 Size: 229 2148 2116 2365 Fragments arranged by size: 2365 2148 2116 229 AflIII A'CryG_T Cuts at: 0 554 1047 1328 1593 3477 4214 6320 6858 Size: 554 493 281 265 1884 737 2106 538 Fragments arranged by size: 2106 1884 737 554 538 493 281 265 AleI CACnn'nnGTG Cuts at: 0 2212 6070 6858 Size: 2212 3858 788 Fragments arranged by size: 3858 2212 788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1943 4078 4582 5988 6858 Size: 1943 2135 504 1406 870 Fragments arranged by size: 2135 1943 1406 870 504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 6412 6444 6858 Size: 6412 32 414 Fragments arranged by size: 6412 414 32 AlwNI CAG_nnn'CTG Cuts at: 0 1362 1387 2736 3676 3882 4675 5199 6654 Size: 1362 25 1349 940 206 793 524 1455 Cuts at: 6654 6858 Size: 204 Fragments arranged by size: 1455 1362 1349 940 793 524 206 204 25 ApaI G_GGCC'C Cuts at: 0 2807 4311 4627 4745 6858 Size: 2807 1504 316 118 2113 Fragments arranged by size: 2807 2113 1504 316 118 ApaLI G'TGCA_C Cuts at: 0 1443 4315 6858 Size: 1443 2872 2543 Fragments arranged by size: 2872 2543 1443 ApoI r'AATT_y Cuts at: 0 2622 4796 4813 5734 6858 Size: 2622 2174 17 921 1124 Fragments arranged by size: 2622 2174 1124 921 17 AvaI C'yCGr_G Cuts at: 0 1121 1639 2111 3250 3910 6858 Size: 1121 518 472 1139 660 2948 Fragments arranged by size: 2948 1139 1121 660 518 472 BamHI G'GATC_C Cuts at: 0 2054 5378 5751 6858 Size: 2054 3324 373 1107 Fragments arranged by size: 3324 2054 1107 373 BanI G'GyrC_C Cuts at: 0 70 1194 2680 4358 4579 4824 6573 6858 Size: 70 1124 1486 1678 221 245 1749 285 Fragments arranged by size: 1749 1678 1486 1124 285 245 221 70 BanII G_rGCy'C Cuts at: 0 1466 1489 2312 2472 2807 2903 3095 3259 Size: 1466 23 823 160 335 96 192 164 Cuts at: 3259 3308 3349 3557 4311 4627 4634 4745 4887 Size: 49 41 208 754 316 7 111 142 Cuts at: 4887 6129 6176 6248 6858 Size: 1242 47 72 610 Fragments arranged by size: 1466 1242 823 754 610 335 316 208 192 164 160 142 111 96 72 49 47 41 23 7 BbeI G_GCGC'C Cuts at: 0 74 1198 2684 4583 6577 6858 Size: 74 1124 1486 1899 1994 281 Fragments arranged by size: 1994 1899 1486 1124 281 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 181 993 1732 2622 2888 3611 4690 6741 Size: 181 812 739 890 266 723 1079 2051 Cuts at: 6741 6858 Size: 117 Fragments arranged by size: 2051 1079 890 812 739 723 266 181 117 BbvCI CC'TCA_GC Cuts at: 0 2366 6043 6858 Size: 2366 3677 815 Fragments arranged by size: 3677 2366 815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 822 856 6422 6456 6571 6605 6858 Size: 822 34 5566 34 115 34 253 Fragments arranged by size: 5566 822 253 115 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 4459 6858 Size: 4459 2399 BclI T'GATC_A Cuts at: 0 5306 6140 6858 Size: 5306 834 718 Fragments arranged by size: 5306 834 718 BfrBI ATG'CAT Cuts at: 0 198 6858 Size: 198 6660 BglI GCCn_nnn'nGGC Cuts at: 0 1285 3025 4025 6115 6552 6858 Size: 1285 1740 1000 2090 437 306 Fragments arranged by size: 2090 1740 1285 1000 437 306 BglII A'GATC_T Cuts at: 0 506 2948 6858 Size: 506 2442 3910 Fragments arranged by size: 3910 2442 506 BlpI GC'TnA_GC Cuts at: 0 2741 2897 4078 4247 6858 Size: 2741 156 1181 169 2611 Fragments arranged by size: 2741 2611 1181 169 156 Bme1580I G_kGCm'C Cuts at: 0 1447 2807 3749 4311 4319 4627 4745 5334 Size: 1447 1360 942 562 8 308 118 589 Cuts at: 5334 6858 Size: 1524 Fragments arranged by size: 1524 1447 1360 942 589 562 308 118 8 BmgBI CAC'GTC Cuts at: 0 1050 1878 3045 5277 6858 Size: 1050 828 1167 2232 1581 Fragments arranged by size: 2232 1581 1167 1050 828 BmrI ACTGGGnnnn_n' Cuts at: 0 1194 3463 4416 5415 6420 6858 Size: 1194 2269 953 999 1005 438 Fragments arranged by size: 2269 1194 1005 999 953 438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 636 2766 3114 4428 5007 6858 Size: 636 2130 348 1314 579 1851 Fragments arranged by size: 2130 1851 1314 636 579 348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1997 2089 3535 3586 3904 4423 4633 5014 Size: 1997 92 1446 51 318 519 210 381 Cuts at: 5014 5203 5317 6041 6325 6832 6841 6858 Size: 189 114 724 284 507 9 17 Fragments arranged by size: 1997 1446 724 519 507 381 318 284 210 189 114 92 51 17 9 Bpu10I CC'TnA_GC Cuts at: 0 231 2366 3904 4628 6043 6858 Size: 231 2135 1538 724 1415 815 Fragments arranged by size: 2135 1538 1415 815 724 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 832 1255 1765 2887 2965 3325 4414 Size: 79 753 423 510 1122 78 360 1089 Cuts at: 4414 6858 Size: 2444 Fragments arranged by size: 2444 1122 1089 753 510 423 360 79 78 BsaI GGTCTCn'nnnn_ Cuts at: 0 1051 1603 3188 4006 4375 5686 6374 6428 Size: 1051 552 1585 818 369 1311 688 54 Cuts at: 6428 6679 6808 6858 Size: 251 129 50 Fragments arranged by size: 1585 1311 1051 818 688 552 369 251 129 54 50 BsaAI yAC'GTr Cuts at: 0 717 1370 1596 1629 2262 3478 4101 5658 Size: 717 653 226 33 633 1216 623 1557 Cuts at: 5658 6321 6453 6858 Size: 663 132 405 Fragments arranged by size: 1557 1216 717 663 653 633 623 405 226 132 33 BsaBI GATnn'nnATC Cuts at: 0 2203 2947 4507 6858 Size: 2203 744 1560 2351 Fragments arranged by size: 2351 2203 1560 744 BsaHI Gr'CG_yC Cuts at: 0 71 1195 2681 4580 6574 6858 Size: 71 1124 1486 1899 1994 284 Fragments arranged by size: 1994 1899 1486 1124 284 71 BsaWI w'CCGG_w Cuts at: 0 759 3329 3815 6773 6858 Size: 759 2570 486 2958 85 Fragments arranged by size: 2958 2570 759 486 85 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1315 1345 2383 2413 3133 3163 3223 3253 Size: 1315 30 1038 30 720 30 60 30 Cuts at: 3253 6412 6442 6858 Size: 3159 30 416 Fragments arranged by size: 3159 1315 1038 720 416 60 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 24 1768 3733 4141 4597 4744 5881 5899 Size: 24 1744 1965 408 456 147 1137 18 Cuts at: 5899 5902 6090 6093 6433 6576 6858 Size: 3 188 3 340 143 282 Fragments arranged by size: 1965 1744 1137 456 408 340 282 188 147 143 24 18 3 3 BseYI C'CCAG_C Cuts at: 0 1840 3408 3666 3942 4370 4570 4769 5402 Size: 1840 1568 258 276 428 200 199 633 Cuts at: 5402 5729 6785 6849 6858 Size: 327 1056 64 9 Fragments arranged by size: 1840 1568 1056 633 428 327 276 258 200 199 64 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1540 3484 4021 5494 6858 Size: 260 1280 1944 537 1473 1364 Fragments arranged by size: 1944 1473 1364 1280 537 260 BsiEI CG_ry'CG Cuts at: 0 1280 2345 5479 6122 6858 Size: 1280 1065 3134 643 736 Fragments arranged by size: 3134 1280 1065 736 643 BsiHKAI G_wGCw'C Cuts at: 0 1447 2903 3095 3349 3557 3627 3905 4319 Size: 1447 1456 192 254 208 70 278 414 Cuts at: 4319 4400 4601 4634 5365 6057 6067 6176 6248 Size: 81 201 33 731 692 10 109 72 Cuts at: 6248 6858 Size: 610 Fragments arranged by size: 1456 1447 731 692 610 414 278 254 208 201 192 109 81 72 70 33 10 BsmBI CGTCTCn'nnnn_ Cuts at: 0 823 2230 6858 Size: 823 1407 4628 Fragments arranged by size: 4628 1407 823 Bsp1286I G_dGCh'C Cuts at: 0 1447 1466 1489 2312 2472 2807 2903 3095 Size: 1447 19 23 823 160 335 96 192 Cuts at: 3095 3259 3308 3349 3557 3627 3749 3905 4311 Size: 164 49 41 208 70 122 156 406 Cuts at: 4311 4319 4400 4601 4627 4634 4745 4887 5334 Size: 8 81 201 26 7 111 142 447 Cuts at: 5334 5365 6057 6067 6129 6176 6248 6858 Size: 31 692 10 62 47 72 610 Fragments arranged by size: 1447 823 692 610 447 406 335 208 201 192 164 160 156 142 122 111 96 81 72 70 62 49 47 41 31 26 23 19 10 8 7 BspEI T'CCGG_A Cuts at: 0 3329 6773 6858 Size: 3329 3444 85 Fragments arranged by size: 3444 3329 85 BspHI T'CATG_A Cuts at: 0 2121 5496 5990 6858 Size: 2121 3375 494 868 Fragments arranged by size: 3375 2121 868 494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3447 4930 4940 5054 6392 6858 Size: 3447 1483 10 114 1338 466 Fragments arranged by size: 3447 1483 1338 466 114 10 BsrBI CCG'CTC Cuts at: 0 14 153 2771 3405 6446 6858 Size: 14 139 2618 634 3041 412 Fragments arranged by size: 3041 2618 634 412 139 14 BsrDI GCAATG_nn' Cuts at: 0 314 6779 6858 Size: 314 6465 79 Fragments arranged by size: 6465 314 79 BsrFI r'CCGG_y Cuts at: 0 1673 4024 6858 Size: 1673 2351 2834 Fragments arranged by size: 2834 2351 1673 BsrGI T'GTAC_A Cuts at: 0 1406 3050 5620 6858 Size: 1406 1644 2570 1238 Fragments arranged by size: 2570 1644 1406 1238 BssHII G'CGCG_C Cuts at: 0 27 6858 Size: 27 6831 BstAPI GCAn_nnn'nTGC Cuts at: 0 2504 3999 5955 6858 Size: 2504 1495 1956 903 Fragments arranged by size: 2504 1956 1495 903 BstBI TT'CG_AA Cuts at: 0 4658 6858 Size: 4658 2200 BstEII G'GTnAC_C Cuts at: 0 1077 6858 Size: 1077 5781 BstXI CCAn_nnnn'nTGG Cuts at: 0 1074 2490 3942 6858 Size: 1074 1416 1452 2916 Fragments arranged by size: 2916 1452 1416 1074 BstYI r'GATC_y Cuts at: 0 411 483 506 710 2054 2198 2948 4502 Size: 411 72 23 204 1344 144 750 1554 Cuts at: 4502 5378 5751 6858 Size: 876 373 1107 Fragments arranged by size: 1554 1344 1107 876 750 411 373 204 144 72 23 Bsu36I CC'TnA_GG Cuts at: 0 1222 6461 6858 Size: 1222 5239 397 Fragments arranged by size: 5239 1222 397 BtgI C'CryG_G Cuts at: 0 1134 1307 1343 1457 1931 2345 2483 2925 Size: 1134 173 36 114 474 414 138 442 Cuts at: 2925 3308 3935 3988 4878 6672 6858 Size: 383 627 53 890 1794 186 Fragments arranged by size: 1794 1134 890 627 474 442 414 383 186 173 138 114 53 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4737 5541 5634 6858 Size: 4737 804 93 1224 Fragments arranged by size: 4737 1224 804 93 BtsI GCAGTG_nn' Cuts at: 0 243 248 2014 4062 4065 6024 6057 6858 Size: 243 5 1766 2048 3 1959 33 801 Fragments arranged by size: 2048 1959 1766 801 243 33 5 3 ClaI AT'CG_AT Cuts at: 0 899 6858 Size: 899 5959 DraIII CAC_nnn'GTG Cuts at: 0 435 477 1499 2319 5174 6858 Size: 435 42 1022 820 2855 1684 Fragments arranged by size: 2855 1684 1022 820 435 42 DrdI GACnn_nn'nnGTC Cuts at: 0 3202 6858 Size: 3202 3656 EaeI y'GGCC_r Cuts at: 0 903 1105 1277 1340 1364 2342 3179 3590 Size: 903 202 172 63 24 978 837 411 Cuts at: 3590 4787 5201 5336 6014 6858 Size: 1197 414 135 678 844 Fragments arranged by size: 1197 978 903 844 837 678 414 411 202 172 135 63 24 EagI C'GGCC_G Cuts at: 0 1277 2342 6858 Size: 1277 1065 4516 Fragments arranged by size: 4516 1277 1065 EarI CTCTTCn'nnn_ Cuts at: 0 605 1228 1745 1784 2152 2213 2854 2993 Size: 605 623 517 39 368 61 641 139 Cuts at: 2993 3354 3562 3682 3865 4501 4774 5017 5380 Size: 361 208 120 183 636 273 243 363 Cuts at: 5380 5548 5888 6522 6858 Size: 168 340 634 336 Fragments arranged by size: 641 636 634 623 605 517 368 363 361 340 336 273 243 208 183 168 139 120 61 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 679 2185 4441 5783 5914 6858 Size: 679 1506 2256 1342 131 944 Fragments arranged by size: 2256 1506 1342 944 679 131 EcoICRI GAG'CTC Cuts at: 0 2901 3093 3347 3555 4632 6174 6246 6858 Size: 2901 192 254 208 1077 1542 72 612 Fragments arranged by size: 2901 1542 1077 612 254 208 192 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 727 1849 1997 2089 3535 3586 3904 Size: 178 549 1122 148 92 1446 51 318 Cuts at: 3904 4423 4633 4702 4798 5014 5176 5203 5317 Size: 519 210 69 96 216 162 27 114 Cuts at: 5317 5358 5404 6041 6325 6685 6832 6841 6858 Size: 41 46 637 284 360 147 9 17 Fragments arranged by size: 1446 1122 637 549 519 360 318 284 216 210 178 162 148 147 114 96 92 69 51 46 41 27 17 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 3454 4615 4930 4981 6858 Size: 3454 1161 315 51 1877 Fragments arranged by size: 3454 1877 1161 315 51 EcoO109I rG'GnC_Cy Cuts at: 0 988 1635 2564 2804 3729 4158 4308 4624 Size: 988 647 929 240 925 429 150 316 Cuts at: 4624 4741 4860 6651 6858 Size: 117 119 1791 207 Fragments arranged by size: 1791 988 929 925 647 429 316 240 207 150 119 117 EcoRI G'AATT_C Cuts at: 0 4813 6858 Size: 4813 2045 EcoRV GAT'ATC Cuts at: 0 1665 6858 Size: 1665 5193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2727 5559 5712 6858 Size: 2727 2832 153 1146 Fragments arranged by size: 2832 2727 1146 153 FseI GG_CCGG'CC Cuts at: 0 4028 6858 Size: 4028 2830 FspI TGC'GCA Cuts at: 0 2461 4555 6858 Size: 2461 2094 2303 Fragments arranged by size: 2461 2303 2094 HaeII r_GCGC'y Cuts at: 0 74 102 231 1198 2379 2684 3177 3419 Size: 74 28 129 967 1181 305 493 242 Cuts at: 3419 3858 4048 4261 4495 4583 4737 4878 5037 Size: 439 190 213 234 88 154 141 159 Cuts at: 5037 5574 5634 6577 6858 Size: 537 60 943 281 Fragments arranged by size: 1181 967 943 537 493 439 305 281 242 234 213 190 159 154 141 129 88 74 60 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 3133 3165 3864 3896 4374 4398 4406 4430 Size: 3133 32 699 32 478 24 8 24 Cuts at: 4430 4700 4732 6858 Size: 270 32 2126 Fragments arranged by size: 3133 2126 699 478 270 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 0 5208 5235 6858 Size: 5208 27 1623 Fragments arranged by size: 5208 1623 27 HindIII A'AGCT_T Cuts at: 0 1816 6858 Size: 1816 5042 KasI G'GCGC_C Cuts at: 0 70 1194 2680 4579 6573 6858 Size: 70 1124 1486 1899 1994 285 Fragments arranged by size: 1994 1899 1486 1124 285 70 MluI A'CGCG_T Cuts at: 0 554 6858 Size: 554 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 623 4196 5030 5933 6102 6587 6858 Size: 623 3573 834 903 169 485 271 Fragments arranged by size: 3573 903 834 623 485 271 169 MscI TGG'CCA Cuts at: 0 905 1107 1342 1366 4789 6858 Size: 905 202 235 24 3423 2069 Fragments arranged by size: 3423 2069 905 235 202 24 MslI CAynn'nnrTG Cuts at: 0 193 1072 1210 2212 2392 2488 5395 6070 Size: 193 879 138 1002 180 96 2907 675 Cuts at: 6070 6598 6697 6858 Size: 528 99 161 Fragments arranged by size: 2907 1002 879 675 528 193 180 161 138 99 96 MspA1I CmG'CkG Cuts at: 0 1716 1992 2067 2244 2415 2927 2940 3840 Size: 1716 276 75 177 171 512 13 900 Cuts at: 3840 4350 4769 5468 6479 6674 6710 6849 6858 Size: 510 419 699 1011 195 36 139 9 Fragments arranged by size: 1716 1011 900 699 512 510 419 276 195 177 171 139 75 36 13 9 NaeI GCC'GGC Cuts at: 0 4026 6858 Size: 4026 2832 NarI GG'CG_CC Cuts at: 0 71 1195 2681 4580 6574 6858 Size: 71 1124 1486 1899 1994 284 Fragments arranged by size: 1994 1899 1486 1124 284 71 NcoI C'CATG_G Cuts at: 0 1457 1931 2483 4878 6858 Size: 1457 474 552 2395 1980 Fragments arranged by size: 2395 1980 1457 552 474 NgoMIV G'CCGG_C Cuts at: 0 4024 6858 Size: 4024 2834 NotI GC'GGCC_GC Cuts at: 0 1277 6858 Size: 1277 5581 NsiI A_TGCA'T Cuts at: 0 200 6858 Size: 200 6658 NspI r_CATG'y Cuts at: 0 198 663 1149 1332 3072 4218 6696 6801 Size: 198 465 486 183 1740 1146 2478 105 Cuts at: 6801 6858 Size: 57 Fragments arranged by size: 2478 1740 1146 486 465 198 183 105 57 PasI CC'CwG_GG Cuts at: 0 3780 6858 Size: 3780 3078 PciI A'CATG_T Cuts at: 0 1328 4214 6858 Size: 1328 2886 2644 Fragments arranged by size: 2886 2644 1328 PflMI CCAn_nnn'nTGG Cuts at: 0 477 1362 2736 6858 Size: 477 885 1374 4122 Fragments arranged by size: 4122 1374 885 477 PfoI T'CCnGG_A Cuts at: 0 1052 2282 3920 3977 4610 4862 6132 6809 Size: 1052 1230 1638 57 633 252 1270 677 Cuts at: 6809 6858 Size: 49 Fragments arranged by size: 1638 1270 1230 1052 677 633 252 57 49 PmlI CAC'GTG Cuts at: 0 717 1596 1629 3478 6858 Size: 717 879 33 1849 3380 Fragments arranged by size: 3380 1849 879 717 33 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1552 1584 6412 6444 6858 Size: 1552 32 4828 32 414 Fragments arranged by size: 4828 1552 414 32 32 PpuMI rG'GwC_Cy Cuts at: 0 3729 4158 4860 6651 6858 Size: 3729 429 702 1791 207 Fragments arranged by size: 3729 1791 702 429 207 PshAI GACnn'nnGTC Cuts at: 0 1615 5105 6858 Size: 1615 3490 1753 Fragments arranged by size: 3490 1753 1615 PspOMI G'GGCC_C Cuts at: 0 2803 4307 4623 4741 6858 Size: 2803 1504 316 118 2117 Fragments arranged by size: 2803 2117 1504 316 118 PspXI vC'TCGA_Gb Cuts at: 0 1639 6858 Size: 1639 5219 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2857 2889 6858 Size: 2857 32 3969 Fragments arranged by size: 3969 2857 32 PstI C_TGCA'G Cuts at: 0 587 1715 1922 2414 2939 3455 3845 4696 Size: 587 1128 207 492 525 516 390 851 Cuts at: 4696 5552 6650 6709 6836 6858 Size: 856 1098 59 127 22 Fragments arranged by size: 1128 1098 856 851 587 525 516 492 390 207 127 59 22 PvuII CAG'CTG Cuts at: 0 2067 2415 2940 3840 4350 4769 6479 6710 Size: 2067 348 525 900 510 419 1710 231 Cuts at: 6710 6849 6858 Size: 139 9 Fragments arranged by size: 2067 1710 900 525 510 419 348 231 139 9 SacI G_AGCT'C Cuts at: 0 2903 3095 3349 3557 4634 6176 6248 6858 Size: 2903 192 254 208 1077 1542 72 610 Fragments arranged by size: 2903 1542 1077 610 254 208 192 72 SacII CC_GC'GG Cuts at: 0 2928 6675 6858 Size: 2928 3747 183 Fragments arranged by size: 3747 2928 183 SapI GCTCTTCn'nnn_ Cuts at: 0 2213 3354 3562 4501 5380 5548 6858 Size: 2213 1141 208 939 879 168 1310 Fragments arranged by size: 2213 1310 1141 939 879 208 168 SbfI CC_TGCA'GG Cuts at: 0 6650 6858 Size: 6650 208 SexAI A'CCwGG_T Cuts at: 0 5447 6359 6653 6858 Size: 5447 912 294 205 Fragments arranged by size: 5447 912 294 205 SfcI C'TryA_G Cuts at: 0 583 1711 1918 2410 2935 3014 3451 3841 Size: 583 1128 207 492 525 79 437 390 Cuts at: 3841 4692 5063 5548 6619 6646 6705 6832 6858 Size: 851 371 485 1071 27 59 127 26 Fragments arranged by size: 1128 1071 851 583 525 492 485 437 390 371 207 127 79 59 27 26 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1285 6858 Size: 1285 5573 SfoI GGC'GCC Cuts at: 0 72 1196 2682 4581 6575 6858 Size: 72 1124 1486 1899 1994 283 Fragments arranged by size: 1994 1899 1486 1124 283 72 SmaI CCC'GGG Cuts at: 0 1123 3912 6858 Size: 1123 2789 2946 Fragments arranged by size: 2946 2789 1123 SmlI C'TyrA_G Cuts at: 0 94 847 1270 1639 1744 2902 2980 3340 Size: 94 753 423 369 105 1158 78 360 Cuts at: 3340 4393 6858 Size: 1053 2465 Fragments arranged by size: 2465 1158 1053 753 423 369 360 105 94 78 SphI G_CATG'C Cuts at: 0 1149 6696 6858 Size: 1149 5547 162 Fragments arranged by size: 5547 1149 162 SrfI GCCC'GGGC Cuts at: 0 1123 6858 Size: 1123 5735 SspI AAT'ATT Cuts at: 0 6770 6858 Size: 6770 88 StuI AGG'CCT Cuts at: 0 1507 1525 2974 3598 4936 5290 6858 Size: 1507 18 1449 624 1338 354 1568 Fragments arranged by size: 1568 1507 1449 1338 624 354 18 StyI C'CwwG_G Cuts at: 0 1283 1457 1931 2210 2483 2834 3020 4575 Size: 1283 174 474 279 273 351 186 1555 Cuts at: 4575 4878 6858 Size: 303 1980 Fragments arranged by size: 1980 1555 1283 474 351 303 279 273 186 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1218 1439 2545 3680 5071 5394 5434 5465 Size: 1218 221 1106 1135 1391 323 40 31 Cuts at: 5465 6757 6858 Size: 1292 101 Fragments arranged by size: 1391 1292 1218 1135 1106 323 221 101 40 31 TatI w'GTAC_w Cuts at: 0 1187 1406 3050 4586 5620 5663 6701 6858 Size: 1187 219 1644 1536 1034 43 1038 157 Fragments arranged by size: 1644 1536 1187 1038 1034 219 157 43 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1315 1347 1437 1469 3973 4005 6858 Size: 1315 32 90 32 2504 32 2853 Fragments arranged by size: 2853 2504 1315 90 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 6655 6858 Size: 6655 203 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 411 1455 5175 5655 6858 Size: 411 1044 3720 480 1203 Fragments arranged by size: 3720 1203 1044 480 411 XhoI C'TCGA_G Cuts at: 0 1639 6858 Size: 1639 5219 XmaI C'CCGG_G Cuts at: 0 1121 3910 6858 Size: 1121 2789 2948 Fragments arranged by size: 2948 2789 1121 XmnI GAAnn'nnTTC Cuts at: 0 2440 5542 5738 5784 5983 6858 Size: 2440 3102 196 46 199 875 Fragments arranged by size: 3102 2440 875 199 196 46 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BmtI BsiWI BsmI BssSI BstZ17I CspCI DraI FspAI HpaI KpnI MfeI NdeI NheI NruI PacI PmeI PsiI PvuI RsrII SalI SanDI ScaI SgfI SgrAI SnaBI SpeI SwaI XbaI ZraI