(Linear) (Six-base) MAPSORT of: KIEE2005.seq Check: 9270 from: 1 to: 1743 KIEE2005.orf With 182 enzymes: SgfI * March 22, 2012 15:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 27 1587 1743 Size: 27 1560 156 Fragments arranged by size: 1560 156 27 AatII G_ACGT'C Cuts at: 0 1172 1743 Size: 1172 571 AccI GT'mk_AC Cuts at: 0 815 1743 Size: 815 928 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 1743 Size: 565 1178 AfeI AGC'GCT Cuts at: 0 673 1743 Size: 673 1070 AflIII A'CryG_T Cuts at: 0 647 1743 Size: 647 1096 AleI CACnn'nnGTG Cuts at: 0 1369 1738 1743 Size: 1369 369 5 Fragments arranged by size: 1369 369 5 ApaI G_GGCC'C Cuts at: 0 16 559 1743 Size: 16 543 1184 Fragments arranged by size: 1184 543 16 ApaLI G'TGCA_C Cuts at: 0 282 1743 Size: 282 1461 AvaI C'yCGr_G Cuts at: 0 9 84 476 483 559 582 775 1242 Size: 9 75 392 7 76 23 193 467 Cuts at: 1242 1743 Size: 501 Fragments arranged by size: 501 467 392 193 76 75 23 9 7 BanI G'GyrC_C Cuts at: 0 447 850 1743 Size: 447 403 893 Fragments arranged by size: 893 447 403 BanII G_rGCy'C Cuts at: 0 16 49 473 484 559 1139 1337 1743 Size: 16 33 424 11 75 580 198 406 Fragments arranged by size: 580 424 406 198 75 33 16 11 BbeI G_GCGC'C Cuts at: 0 451 854 1743 Size: 451 403 889 Fragments arranged by size: 889 451 403 BbsI GAAGACnn'nnnn_ Cuts at: 0 927 1099 1288 1743 Size: 927 172 189 455 Fragments arranged by size: 927 455 189 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 114 148 1743 Size: 114 34 1595 Fragments arranged by size: 1595 114 34 BclI T'GATC_A Cuts at: 0 1649 1743 Size: 1649 94 BglI GCCn_nnn'nGGC Cuts at: 0 516 700 1084 1743 Size: 516 184 384 659 Fragments arranged by size: 659 516 384 184 BglII A'GATC_T Cuts at: 0 1505 1520 1743 Size: 1505 15 223 Fragments arranged by size: 1505 223 15 Bme1580I G_kGCm'C Cuts at: 0 16 286 559 1743 Size: 16 270 273 1184 Fragments arranged by size: 1184 273 270 16 BmgBI CAC'GTC Cuts at: 0 648 1229 1743 Size: 648 581 514 Fragments arranged by size: 648 581 514 BmrI ACTGGGnnnn_n' Cuts at: 0 1719 1743 Size: 1719 24 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 312 1743 Size: 312 1431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 705 931 1330 1743 Size: 675 30 226 399 413 Fragments arranged by size: 675 413 399 226 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1743 Size: 328 1415 BsaI GGTCTCn'nnnn_ Cuts at: 0 300 1743 Size: 300 1443 BsaAI yAC'GTr Cuts at: 0 600 1155 1743 Size: 600 555 588 Fragments arranged by size: 600 588 555 BsaHI Gr'CG_yC Cuts at: 0 448 851 1169 1743 Size: 448 403 318 574 Fragments arranged by size: 574 448 403 318 BsaWI w'CCGG_w Cuts at: 0 1268 1743 Size: 1268 475 BseYI C'CCAG_C Cuts at: 0 1679 1743 Size: 1679 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 615 909 1623 1743 Size: 615 294 714 120 Fragments arranged by size: 714 615 294 120 BsiEI CG_ry'CG Cuts at: 0 511 873 1743 Size: 511 362 870 Fragments arranged by size: 870 511 362 BsiHKAI G_wGCw'C Cuts at: 0 265 286 352 539 740 919 1139 1196 Size: 265 21 66 187 201 179 220 57 Cuts at: 1196 1743 Size: 547 Fragments arranged by size: 547 265 220 201 187 179 66 57 21 Bsp1286I G_dGCh'C Cuts at: 0 16 49 265 286 352 473 484 539 Size: 16 33 216 21 66 121 11 55 Cuts at: 539 559 740 919 1139 1196 1337 1743 Size: 20 181 179 220 57 141 406 Fragments arranged by size: 406 220 216 181 179 141 121 66 57 55 33 21 20 16 11 BspEI T'CCGG_A Cuts at: 0 1268 1743 Size: 1268 475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 27 282 1587 1743 Size: 27 255 1305 156 Fragments arranged by size: 1305 255 156 27 BsrBI CCG'CTC Cuts at: 0 627 1743 Size: 627 1116 BsrFI r'CCGG_y Cuts at: 0 847 919 1012 1183 1359 1638 1743 Size: 847 72 93 171 176 279 105 Fragments arranged by size: 847 279 176 171 105 93 72 BsrGI T'GTAC_A Cuts at: 0 1349 1743 Size: 1349 394 BssHII G'CGCG_C Cuts at: 0 527 620 1743 Size: 527 93 1123 Fragments arranged by size: 1123 527 93 BssSI C'ACGA_G Cuts at: 0 48 172 205 1030 1743 Size: 48 124 33 825 713 Fragments arranged by size: 825 713 124 48 33 BstAPI GCAn_nnn'nTGC Cuts at: 0 866 923 1743 Size: 866 57 820 Fragments arranged by size: 866 820 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 75 165 1743 Size: 75 90 1578 Fragments arranged by size: 1578 90 75 BstYI r'GATC_y Cuts at: 0 113 1418 1505 1520 1715 1743 Size: 113 1305 87 15 195 28 Fragments arranged by size: 1305 195 113 87 28 15 BtgI C'CryG_G Cuts at: 0 68 237 821 1499 1701 1743 Size: 68 169 584 678 202 42 Fragments arranged by size: 678 584 202 169 68 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 846 1743 Size: 846 897 BtsI GCAGTG_nn' Cuts at: 0 686 1743 Size: 686 1057 DraIII CAC_nnn'GTG Cuts at: 0 600 1743 Size: 600 1143 DrdI GACnn_nn'nnGTC Cuts at: 0 1584 1743 Size: 1584 159 EaeI y'GGCC_r Cuts at: 0 240 508 692 882 962 1007 1361 1481 Size: 240 268 184 190 80 45 354 120 Cuts at: 1481 1640 1743 Size: 159 103 Fragments arranged by size: 354 268 240 190 184 159 120 103 80 45 EagI C'GGCC_G Cuts at: 0 508 1743 Size: 508 1235 EarI CTCTTCn'nnn_ Cuts at: 0 601 847 1342 1618 1743 Size: 601 246 495 276 125 Fragments arranged by size: 601 495 276 246 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 826 1743 Size: 826 917 EcoICRI GAG'CTC Cuts at: 0 1137 1743 Size: 1137 606 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 675 705 931 1330 1743 Size: 565 110 30 226 399 413 Fragments arranged by size: 565 413 399 226 110 30 EcoO109I rG'GnC_Cy Cuts at: 0 13 555 556 1743 Size: 13 542 1 1187 Fragments arranged by size: 1187 542 13 1 FspI TGC'GCA Cuts at: 0 1117 1743 Size: 1117 626 HaeII r_GCGC'y Cuts at: 0 9 84 451 675 728 783 854 1743 Size: 9 75 367 224 53 55 71 889 Fragments arranged by size: 889 367 224 75 71 55 53 9 HincII GTy'rAC Cuts at: 0 1173 1743 Size: 1173 570 KasI G'GCGC_C Cuts at: 0 447 850 1743 Size: 447 403 893 Fragments arranged by size: 893 447 403 MscI TGG'CCA Cuts at: 0 694 964 1743 Size: 694 270 779 Fragments arranged by size: 779 694 270 MslI CAynn'nnrTG Cuts at: 0 73 1369 1738 1743 Size: 73 1296 369 5 Fragments arranged by size: 1296 369 73 5 MspA1I CmG'CkG Cuts at: 0 219 501 516 543 881 1553 1658 1743 Size: 219 282 15 27 338 672 105 85 Fragments arranged by size: 672 338 282 219 105 85 27 15 NaeI GCC'GGC Cuts at: 0 849 1014 1361 1743 Size: 849 165 347 382 Fragments arranged by size: 849 382 347 165 NarI GG'CG_CC Cuts at: 0 448 851 1743 Size: 448 403 892 Fragments arranged by size: 892 448 403 NcoI C'CATG_G Cuts at: 0 68 1743 Size: 68 1675 NgoMIV G'CCGG_C Cuts at: 0 847 1012 1359 1743 Size: 847 165 347 384 Fragments arranged by size: 847 384 347 165 PflMI CCAn_nnn'nTGG Cuts at: 0 600 1743 Size: 600 1143 PfoI T'CCnGG_A Cuts at: 0 899 1743 Size: 899 844 PmlI CAC'GTG Cuts at: 0 600 1743 Size: 600 1143 PspOMI G'GGCC_C Cuts at: 0 12 555 1743 Size: 12 543 1188 Fragments arranged by size: 1188 543 12 PstI C_TGCA'G Cuts at: 0 38 1586 1743 Size: 38 1548 157 Fragments arranged by size: 1548 157 38 SacI G_AGCT'C Cuts at: 0 1139 1743 Size: 1139 604 SapI GCTCTTCn'nnn_ Cuts at: 0 601 1342 1618 1743 Size: 601 741 276 125 Fragments arranged by size: 741 601 276 125 SbfI CC_TGCA'GG Cuts at: 0 38 1743 Size: 38 1705 SexAI A'CCwGG_T Cuts at: 0 997 1214 1743 Size: 997 217 529 Fragments arranged by size: 997 529 217 SfcI C'TryA_G Cuts at: 0 34 1582 1743 Size: 34 1548 161 Fragments arranged by size: 1548 161 34 SfiI GGCCn_nnn'nGGCC Cuts at: 0 516 1743 Size: 516 1227 SfoI GGC'GCC Cuts at: 0 449 852 1743 Size: 449 403 891 Fragments arranged by size: 891 449 403 SgrAI Cr'CCGG_yG Cuts at: 0 847 1743 Size: 847 896 SmaI CCC'GGG Cuts at: 0 11 86 478 561 584 1743 Size: 11 75 392 83 23 1159 Fragments arranged by size: 1159 392 83 75 23 11 SmlI C'TyrA_G Cuts at: 0 343 1743 Size: 343 1400 SrfI GCCC'GGGC Cuts at: 0 11 86 1743 Size: 11 75 1657 Fragments arranged by size: 1657 75 11 StyI C'CwwG_G Cuts at: 0 68 1743 Size: 68 1675 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 969 1743 Size: 969 774 TatI w'GTAC_w Cuts at: 0 650 1349 1743 Size: 650 699 394 Fragments arranged by size: 699 650 394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 66 1104 1497 1743 Size: 66 1038 393 246 Fragments arranged by size: 1038 393 246 66 XmaI C'CCGG_G Cuts at: 0 9 84 476 559 582 1743 Size: 9 75 392 83 23 1161 Fragments arranged by size: 1161 392 83 75 23 9 ZraI GAC'GTC Cuts at: 0 1170 1743 Size: 1170 573 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AleI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI PmlI PspOMI PstI SacI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BlpI BmtI Bpu10I BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspHI BsrDI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SgfI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI