################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BlnI BsaAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MfeI MluI MunI NcoI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 1327 GTCTAC GT^[AC][GT]AC AccIII 561 TCCGGA T^CCGGA AfaI 1371 GTAC GT^AC AflIII 776 ACATGT A^C[GA][CT]GT AseI 1303 ATTAAT AT^TAAT AsnI 1303 ATTAAT AT^TAAT BcgI 1392 CGAGATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 303 CCGGG CC^[GC]GG BfaI 572 CTAG C^TAG BglII 1394 AGATCT A^GATCT BlpI 321 GCTGAGC GC^T[ACGT]AGC BpmI 136 CTGGAG CTGGAG(16/14) Bpu1102I 321 GCTGAGC GC^T[ACGT]AGC BsaBI 1380 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1682 GAGACC GGTCTC(1/5) BsaWI 561 TCCGGA [AT]^CCGG[AT] BseAI 561 TCCGGA T^CCGGA BseRI 1079 CTCCTC GAGGAG(10/8) BsgI 420 CTGCAC GTGCAG(16/14) BsiEI 65 CGGCCG CG[GA][CT]^CG BspEI 561 TCCGGA T^CCGGA BspLU11I 776 ACATGT A^CATGT BsrBI 28 GAGCGG CCGCTC(-3/-3) BsrDI 636 CATTGC GCAATG(2/0) BstAPI 1368 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 831 CCAGAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1394 AGATCT [GA]^GATC[CT] CelII 321 GCTGAGC GC^T[ACGT]AGC DrdI 1153 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 65 CGGCCG C^GGCCG EclXI 65 CGGCCG C^GGCCG Eco52I 65 CGGCCG C^GGCCG KspI 68 CCGCGG CCGC^GG MaeI 572 CTAG C^TAG MamI 1380 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1394 AGATCT [GA]^GATC[CT] MroI 561 TCCGGA T^CCGGA MslI 771 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 95 GCCGGC GCC^GGC NciI 303 CCGGG CC^[GC]GG NdeI 1022 CATATG CA^TATG NgoMIV 95 GCCGGC G^CCGGC NotI 64 GCGGCCGC GC^GGCCGC PflMI 1541 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1303 ATTAAT AT^TAAT PvuII 1433 CAGCTG CAG^CTG RsaI 1371 GTAC GT^AC SacII 68 CCGCGG CCGC^GG SapI 237 GCTCTTC GCTCTTC(1/4) ScaI 1370 AGTACT AGT^ACT SphI 347 GCATGC GCATG^C StuI 1413 AGGCCT AGG^CCT Van91I 1541 CCATCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 810 CCAGAAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1394 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 1123 GTGCTC G[AT]GC[AT]^C 1239 GTGCTC AvaII 433 GGTCC G^G[AT]CC 559 GGTCC BalI 503 TGGCCA TGG^CCA 726 TGGCCA BanII 6 GGGCTC G[GA]GC[CT]^C 261 GGGCTC BglI 67 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 220 GCCAGCGAGGC BsiHKAI 1123 GTGCTC G[AT]GC[AT]^C 1239 GTGCTC BsmAI 1682 GAGAC GTCTC(1/5) 903 GTCTC BsmFI 434 GTCCC GGGAC(10/14) 964 GTCCC BspMI 338 ACCTGC ACCTGC(4/8) 1359 GCAGGT BsrFI 95 GCCGGC [GA]^CCGG[CT] 462 ACCGGC Cfr10I 95 GCCGGC [GA]^CCGG[CT] 462 ACCGGC DdeI 322 CTGAG C^T[ACGT]AG 1431 CTCAG DsaI 68 CCGCGG C^C[GA][CT]GG 1217 CCGTGG MaeIII 667 GTCAC ^GT[ACGT]AC 1559 GTCAC MluNI 503 TGGCCA TGG^CCA 726 TGGCCA MscI 503 TGGCCA TGG^CCA 726 TGGCCA PleI 854 GAGTC GAGTC(4/5) 1035 GAGTC PstI 1094 CTGCAG CTGCA^G 1253 CTGCAG SexAI 170 ACCTGGT A^CC[AT]GGT 542 ACCAGGT SfaNI 1117 GATGC GCATC(5/9) 158 GCATC SfcI 1094 CTGCAG C^T[GA][CT]AG 1253 CTGCAG SspI 1066 AATATT AAT^ATT 1576 AATATT TfiI 889 GATTC G^A[AT]TC 1050 GATTC Tsp45I 667 GTCAC ^GT[GC]AC 1559 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwI ApoI BanI BbeI BbsI BbvI BmyI BpuAI BsaHI BsaJI BsiYI BslI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfoI TaqI Tru9I TseI Tsp509I TspRI TthHB8I