(Circular) (Six-base) MAPSORT of: pFN21AE2787.seq Check: 9910 from: 1 to: 6436 pFN21AE2787 6436 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3355 3355 Size: 6436 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3333 3779 1937 Size: 1396 446 4594 Fragments arranged by size: 4594 1396 446 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 3230 4499 5883 6151 1632 Size: 1541 57 1269 1384 268 1917 Fragments arranged by size: 1917 1541 1384 1269 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 2781 5335 6312 1203 Size: 1578 2554 977 1327 Fragments arranged by size: 2554 1578 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 4179 5751 4179 Size: 1572 4864 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 3063 3264 3425 3745 3971 4123 6232 1493 Size: 1570 201 161 320 226 152 2109 1697 Fragments arranged by size: 2109 1697 1570 320 226 201 161 152 AseI AT'TA_AT Cuts at: 160 1032 3309 4990 160 Size: 872 2277 1681 1606 Fragments arranged by size: 2277 1681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 3761 1870 Size: 73 1818 4545 Fragments arranged by size: 4545 1818 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2783 2816 2783 Size: 33 6403 BamHI G'GATC_C Cuts at: 3766 4133 3766 Size: 367 6069 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2197 2431 2540 3367 Size: 325 217 627 251 159 234 109 827 Cuts at: 3367 3757 5212 618 Size: 390 1455 1842 Fragments arranged by size: 1842 1455 827 627 390 325 251 234 217 159 109 BanII G_rGCy'C Cuts at: 1391 2015 2270 3755 1391 Size: 624 255 1485 4072 Fragments arranged by size: 4072 1485 624 255 BbeI G_GCGC'C Cuts at: 2042 2201 2435 2544 2042 Size: 159 234 109 5934 Fragments arranged by size: 5934 234 159 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2713 3035 3190 928 Size: 971 814 322 155 4174 Fragments arranged by size: 4174 971 814 322 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3386 3420 4627 4661 1903 Size: 34 1449 34 1207 34 3678 Fragments arranged by size: 3678 1449 1207 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2231 5047 Size: 107 122 71 631 605 406 153 2816 Cuts at: 5047 136 Size: 1525 Fragments arranged by size: 2816 1525 631 605 406 153 122 107 71 BglII A'GATC_T Cuts at: 3399 6431 3399 Size: 3032 3404 BlpI GC'TnA_GC Cuts at: 2327 3853 2327 Size: 1526 4910 Bme1580I G_kGCm'C Cuts at: 1790 3720 4497 5653 1790 Size: 1930 777 1156 2573 Fragments arranged by size: 2573 1930 1156 777 BmgBI CAC'GTC Cuts at: 1318 2169 1318 Size: 851 5585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4167 5125 442 Size: 416 53 83 399 24 2750 958 1753 Fragments arranged by size: 2750 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 3659 2414 Size: 1245 5191 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 5096 1239 Size: 923 2934 2579 Fragments arranged by size: 2934 2579 923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4561 5426 5724 4561 Size: 865 298 5273 Fragments arranged by size: 5273 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3681 5099 882 Size: 617 2182 1418 2219 Fragments arranged by size: 2219 2182 1418 617 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 3389 6430 3389 Size: 3041 3395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 2198 2432 2445 Size: 53 83 186 936 506 159 234 13 Cuts at: 2445 2541 3495 4243 4625 5260 275 Size: 96 954 748 382 635 1451 Fragments arranged by size: 1451 954 936 748 635 506 382 234 186 159 96 83 53 13 BsaWI w'CCGG_w Cuts at: 2566 4869 5267 5541 5688 6099 2566 Size: 2303 398 274 147 411 2903 Fragments arranged by size: 2903 2303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2740 2770 3067 3097 1178 Size: 30 165 30 1337 30 297 30 4517 Fragments arranged by size: 4517 1337 297 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3075 1186 Size: 1889 4547 BseYI C'CCAG_C Cuts at: 2047 2084 2208 2302 2347 5639 6135 2047 Size: 37 124 94 45 3292 496 2348 Fragments arranged by size: 3292 2348 496 124 94 45 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2410 1163 Size: 573 674 5189 Fragments arranged by size: 5189 674 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3886 4647 5675 1540 Size: 275 186 72 1813 761 1028 2301 Fragments arranged by size: 2301 1813 1028 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 3132 3248 4497 4582 5653 3132 Size: 116 1249 85 1071 3915 Fragments arranged by size: 3915 1249 1071 116 85 BsmI GAATG_Cn' Cuts at: 3947 4040 3947 Size: 93 6343 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2270 3132 3248 3720 3755 4497 Size: 399 225 255 862 116 472 35 742 Cuts at: 4497 4582 5653 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 862 742 472 399 255 225 116 85 35 BspEI T'CCGG_A Cuts at: 2566 6099 2566 Size: 3533 2903 BspHI T'CATG_A Cuts at: 4325 6055 4325 Size: 1730 4706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 3355 3791 844 Size: 1508 1003 436 3489 Fragments arranged by size: 3489 1508 1003 436 BsrBI CCG'CTC Cuts at: 1394 1823 2035 4323 1394 Size: 429 212 2288 3507 Fragments arranged by size: 3507 2288 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2640 4931 5105 65 Size: 1183 1392 2291 174 1396 Fragments arranged by size: 2291 1396 1392 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 2467 5080 5267 1700 Size: 110 290 367 2613 187 2869 Fragments arranged by size: 2869 2613 367 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 2052 2104 2146 2217 2312 2314 2411 1925 Size: 127 52 42 71 95 2 97 5950 Fragments arranged by size: 5950 127 97 95 71 52 42 2 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 3379 794 Size: 2585 3851 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstXI CCAn_nnnn'nTGG Cuts at: 2843 2843 Size: 6436 BstYI r'GATC_y Cuts at: 1972 3399 3766 4133 4518 4535 5987 6073 6085 Size: 1427 367 367 385 17 1452 86 12 Cuts at: 6085 6431 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1427 385 367 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 3222 513 Size: 552 939 69 1149 3727 Fragments arranged by size: 3727 1149 939 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 2773 3952 4746 4766 2773 Size: 1179 794 20 4443 Fragments arranged by size: 4443 1179 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3164 5443 809 Size: 507 1848 2279 1802 Fragments arranged by size: 2279 1848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2289 2373 Size: 54 1425 50 275 18 240 219 84 Cuts at: 2373 2469 2508 2731 3883 4772 6401 8 Size: 96 39 223 1152 889 1629 43 Fragments arranged by size: 1629 1425 1152 889 275 240 223 219 96 84 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 3883 1537 Size: 275 258 1813 4090 Fragments arranged by size: 4090 1813 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2925 3206 4366 1353 Size: 896 676 281 1160 3423 Fragments arranged by size: 3423 1160 896 676 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 3173 3230 4499 5096 5883 6151 Size: 393 530 1011 57 1269 597 787 268 Cuts at: 6151 1239 Size: 1524 Fragments arranged by size: 1524 1269 1011 787 597 530 393 268 57 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 2042 2201 2237 2339 2435 2544 2836 5583 Size: 364 159 36 102 96 109 292 2747 Cuts at: 5583 1678 Size: 2531 Fragments arranged by size: 2747 2531 364 292 159 109 102 96 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3547 3579 5077 5109 5151 Size: 32 135 32 1975 32 1498 32 42 Cuts at: 5151 5183 1373 Size: 32 2626 Fragments arranged by size: 2626 1975 1498 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KasI G'GCGC_C Cuts at: 2038 2197 2431 2540 2038 Size: 159 234 109 5934 Fragments arranged by size: 5934 234 159 109 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 5550 5734 1305 Size: 891 3354 184 2007 Fragments arranged by size: 3354 2007 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 2733 10 Size: 54 1425 343 678 223 3713 Fragments arranged by size: 3713 1425 678 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 2780 4394 4753 4912 518 Size: 648 1614 1614 359 159 2042 Fragments arranged by size: 2042 1614 1614 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2075 2368 2457 3440 3852 4529 5677 Size: 438 31 293 89 983 412 677 1148 Cuts at: 5677 5922 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 983 677 438 412 293 245 89 31 NaeI GCC'GGC Cuts at: 2102 2102 Size: 6436 NarI GG'CG_CC Cuts at: 2039 2198 2432 2541 2039 Size: 159 234 109 5934 Fragments arranged by size: 5934 234 159 109 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4945 Fragments arranged by size: 4945 939 552 NdeI CA'TA_TG Cuts at: 387 3028 387 Size: 2641 3795 NgoMIV G'CCGG_C Cuts at: 2100 2100 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 2070 3883 2070 Size: 1813 4623 NspI r_CATG'y Cuts at: 1132 1229 2356 2785 3232 3794 5339 1132 Size: 97 1127 429 447 562 1545 2229 Fragments arranged by size: 2229 1545 1127 562 447 429 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 2781 5335 2781 Size: 2554 3882 PflMI CCAn_nnn'nTGG Cuts at: 1108 3552 1108 Size: 2444 3992 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PstI C_TGCA'G Cuts at: 830 3103 3262 3788 830 Size: 2273 159 526 3478 Fragments arranged by size: 3478 2273 526 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 3440 3440 Size: 6436 SacII CC_GC'GG Cuts at: 2076 2076 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SapI GCTCTTCn'nnn_ Cuts at: 2249 2249 Size: 6436 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 3377 4684 6396 3377 Size: 1307 1712 3417 Fragments arranged by size: 3417 1712 1307 SexAI A'CCwGG_T Cuts at: 2175 2547 2175 Size: 372 6064 SfcI C'TryA_G Cuts at: 826 1045 3099 3258 3784 4919 5600 5791 826 Size: 219 2054 159 526 1135 681 191 1471 Fragments arranged by size: 2054 1471 1135 681 526 219 191 159 SfoI GGC'GCC Cuts at: 2040 2199 2433 2542 2040 Size: 159 234 109 5934 Fragments arranged by size: 5934 234 159 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3763 1872 Size: 1891 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4540 5441 5703 820 Size: 197 926 2597 901 262 1553 Fragments arranged by size: 2597 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 2356 3794 1132 Size: 1224 1438 3774 Fragments arranged by size: 3774 1438 1224 SspI AAT'ATT Cuts at: 5 52 3073 3583 4360 5 Size: 47 3021 510 777 2081 Fragments arranged by size: 3021 2081 777 510 47 StuI AGG'CCT Cuts at: 1517 3420 1517 Size: 1903 4533 StyI C'CwwG_G Cuts at: 513 1065 2004 3875 513 Size: 552 939 1871 3074 Fragments arranged by size: 3074 1871 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4463 4480 4633 4818 5263 325 Size: 1255 2883 17 153 185 445 1498 Fragments arranged by size: 2883 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3375 4682 6394 Size: 274 80 33 51 1443 1398 1307 1712 Cuts at: 6394 96 Size: 138 Fragments arranged by size: 1712 1443 1398 1307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2740 2772 6396 6428 2740 Size: 32 3624 32 2748 Fragments arranged by size: 3624 2748 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2822 2822 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 3761 1870 Size: 1891 4545 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI