(Linear) (Six-base) MAPSORT of: KIEE2787.seq Check: 8981 from: 1 to: 1731 KIEE2787.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1350 1731 Size: 1350 381 AccI GT'mk_AC Cuts at: 0 1328 1731 Size: 1328 403 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1225 1731 Size: 1168 57 506 Fragments arranged by size: 1168 506 57 AflIII A'CryG_T Cuts at: 0 776 1731 Size: 776 955 ApoI r'AATT_y Cuts at: 0 1058 1259 1420 1731 Size: 1058 201 161 311 Fragments arranged by size: 1058 311 201 161 AseI AT'TA_AT Cuts at: 0 1304 1731 Size: 1304 427 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 778 811 1731 Size: 778 33 920 Fragments arranged by size: 920 778 33 BanI G'GyrC_C Cuts at: 0 33 192 426 535 1362 1731 Size: 33 159 234 109 827 369 Fragments arranged by size: 827 369 234 159 109 33 BanII G_rGCy'C Cuts at: 0 10 265 1731 Size: 10 255 1466 Fragments arranged by size: 1466 255 10 BbeI G_GCGC'C Cuts at: 0 37 196 430 539 1731 Size: 37 159 234 109 1192 Fragments arranged by size: 1192 234 159 109 37 BbsI GAAGACnn'nnnn_ Cuts at: 0 708 1030 1185 1731 Size: 708 322 155 546 Fragments arranged by size: 708 546 322 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1381 1415 1731 Size: 1381 34 316 Fragments arranged by size: 1381 316 34 BglI GCCn_nnn'nGGC Cuts at: 0 73 226 1731 Size: 73 153 1505 Fragments arranged by size: 1505 153 73 BglII A'GATC_T Cuts at: 0 1394 1731 Size: 1394 337 BlpI GC'TnA_GC Cuts at: 0 322 1731 Size: 322 1409 Bme1580I G_kGCm'C Cuts at: 0 1715 1731 Size: 1715 16 BmgBI CAC'GTC Cuts at: 0 164 1731 Size: 164 1567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 409 1654 1731 Size: 409 1245 77 Fragments arranged by size: 1245 409 77 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 1731 Size: 157 1574 BsaI GGTCTCn'nnnn_ Cuts at: 0 1676 1731 Size: 1676 55 BsaBI GATnn'nnATC Cuts at: 0 1384 1731 Size: 1384 347 BsaHI Gr'CG_yC Cuts at: 0 34 193 427 440 536 1490 1731 Size: 34 159 234 13 96 954 241 Fragments arranged by size: 954 241 234 159 96 34 13 BsaWI w'CCGG_w Cuts at: 0 561 1731 Size: 561 1170 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 735 765 1062 1092 1731 Size: 735 30 297 30 639 Fragments arranged by size: 735 639 297 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1070 1731 Size: 1070 661 BseYI C'CCAG_C Cuts at: 0 42 79 203 297 342 1731 Size: 42 37 124 94 45 1389 Fragments arranged by size: 1389 124 94 45 42 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1731 Size: 405 1326 BsiEI CG_ry'CG Cuts at: 0 68 1731 Size: 68 1663 BsiHKAI G_wGCw'C Cuts at: 0 1127 1243 1731 Size: 1127 116 488 Fragments arranged by size: 1127 488 116 Bsp1286I G_dGCh'C Cuts at: 0 10 265 1127 1243 1715 1731 Size: 10 255 862 116 472 16 Fragments arranged by size: 862 472 255 116 16 10 BspEI T'CCGG_A Cuts at: 0 561 1731 Size: 561 1170 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 347 1350 1731 Size: 347 1003 381 Fragments arranged by size: 1003 381 347 BsrBI CCG'CTC Cuts at: 0 30 1731 Size: 30 1701 BsrDI GCAATG_nn' Cuts at: 0 635 1731 Size: 635 1096 BsrFI r'CCGG_y Cuts at: 0 95 462 1731 Size: 95 367 1269 Fragments arranged by size: 1269 367 95 BssHII G'CGCG_C Cuts at: 0 47 99 141 212 307 309 406 1731 Size: 47 52 42 71 95 2 97 1325 Fragments arranged by size: 1325 97 95 71 52 47 42 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1374 1731 Size: 1374 357 BstXI CCAn_nnnn'nTGG Cuts at: 0 838 1731 Size: 838 893 BstYI r'GATC_y Cuts at: 0 1394 1731 Size: 1394 337 BtgI C'CryG_G Cuts at: 0 68 1217 1731 Size: 68 1149 514 Fragments arranged by size: 1149 514 68 BtsI GCAGTG_nn' Cuts at: 0 768 1731 Size: 768 963 DrdI GACnn_nn'nnGTC Cuts at: 0 1159 1731 Size: 1159 572 EaeI y'GGCC_r Cuts at: 0 65 284 368 464 503 726 1731 Size: 65 219 84 96 39 223 1005 Fragments arranged by size: 1005 223 219 96 84 65 39 EagI C'GGCC_G Cuts at: 0 65 1731 Size: 65 1666 EarI CTCTTCn'nnn_ Cuts at: 0 244 920 1201 1731 Size: 244 676 281 530 Fragments arranged by size: 676 530 281 244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 1168 1225 1731 Size: 157 1011 57 506 Fragments arranged by size: 1011 506 157 57 HaeII r_GCGC'y Cuts at: 0 37 196 232 334 430 539 831 1731 Size: 37 159 36 102 96 109 292 900 Fragments arranged by size: 900 292 159 109 102 96 37 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1542 1574 1731 Size: 1542 32 157 Fragments arranged by size: 1542 157 32 KasI G'GCGC_C Cuts at: 0 33 192 426 535 1731 Size: 33 159 234 109 1196 Fragments arranged by size: 1196 234 159 109 33 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 191 1731 Size: 191 1540 MscI TGG'CCA Cuts at: 0 505 728 1731 Size: 505 223 1003 Fragments arranged by size: 1003 505 223 MslI CAynn'nnrTG Cuts at: 0 775 1731 Size: 775 956 MspA1I CmG'CkG Cuts at: 0 39 70 363 452 1435 1731 Size: 39 31 293 89 983 296 Fragments arranged by size: 983 296 293 89 39 31 NaeI GCC'GGC Cuts at: 0 97 1731 Size: 97 1634 NarI GG'CG_CC Cuts at: 0 34 193 427 536 1731 Size: 34 159 234 109 1195 Fragments arranged by size: 1195 234 159 109 34 NdeI CA'TA_TG Cuts at: 0 1023 1731 Size: 1023 708 NgoMIV G'CCGG_C Cuts at: 0 95 1731 Size: 95 1636 NotI GC'GGCC_GC Cuts at: 0 65 1731 Size: 65 1666 NspI r_CATG'y Cuts at: 0 351 780 1227 1731 Size: 351 429 447 504 Fragments arranged by size: 504 447 429 351 PciI A'CATG_T Cuts at: 0 776 1731 Size: 776 955 PflMI CCAn_nnn'nTGG Cuts at: 0 1547 1731 Size: 1547 184 PstI C_TGCA'G Cuts at: 0 1098 1257 1731 Size: 1098 159 474 Fragments arranged by size: 1098 474 159 PvuII CAG'CTG Cuts at: 0 1435 1731 Size: 1435 296 SacII CC_GC'GG Cuts at: 0 71 1731 Size: 71 1660 SapI GCTCTTCn'nnn_ Cuts at: 0 244 1731 Size: 244 1487 ScaI AGT'ACT Cuts at: 0 1372 1731 Size: 1372 359 SexAI A'CCwGG_T Cuts at: 0 170 542 1731 Size: 170 372 1189 Fragments arranged by size: 1189 372 170 SfcI C'TryA_G Cuts at: 0 1094 1253 1731 Size: 1094 159 478 Fragments arranged by size: 1094 478 159 SfoI GGC'GCC Cuts at: 0 35 194 428 537 1731 Size: 35 159 234 109 1194 Fragments arranged by size: 1194 234 159 109 35 SphI G_CATG'C Cuts at: 0 351 1731 Size: 351 1380 SspI AAT'ATT Cuts at: 0 1068 1578 1731 Size: 1068 510 153 Fragments arranged by size: 1068 510 153 StuI AGG'CCT Cuts at: 0 1415 1731 Size: 1415 316 TatI w'GTAC_w Cuts at: 0 1370 1731 Size: 1370 361 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 735 767 1731 Size: 735 32 964 Fragments arranged by size: 964 735 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 817 1731 Size: 817 914 Enzymes that do cut: AarI AccI AcuI AflIII ApoI AseI BaeI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BplI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI Eco57MI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NotI NspI PciI PflMI PstI PvuII SacII SapI ScaI SexAI SfcI SfoI SphI SspI StuI TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BbvCI BciVI BclI BfrBI BmrI BmtI Bpu10I BpuEI BsaAI BsiWI BsmI BsmBI BspHI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NheI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI