################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BbeI BcgI BfrI BglI BglII BlnI BsaBI BsiEI BsiHKAI BsiWI BsmBI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 14 TCCGGA T^CCGGA AclI 424 AACGTT AA^CGTT AcyI 2356 GGCGTC G[GA]^CG[CT]C AocI 2259 CCTGAGG CC^T[ACGT]AGG AspI 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 2500 GGCACC G^G[CT][GA]CC BanII 1032 GGGCTC G[GA]GC[CT]^C BbrPI 742 CACGTG CAC^GTG BbsI 634 GAAGAC GAAGAC(2/6) BciVI 1795 GGATAC GTATCC(6/5) BclI 2417 TGATCA T^GATCA BlpI 2274 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2274 GCTTAGC GC^T[ACGT]AGC BpuAI 634 GAAGAC GAAGAC(2/6) BsaAI 742 CACGTG [CT]AC^GT[GA] BsaHI 2356 GGCGTC G[GA]^CG[CT]C BseAI 14 TCCGGA T^CCGGA BspEI 14 TCCGGA T^CCGGA BspMI 1010 ACCTGC ACCTGC(4/8) BsrBI 772 GAGCGG CCGCTC(-3/-3) BsrFI 1349 GCCGGT [GA]^CCGG[CT] BstAPI 1005 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 26 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2259 CCTGAGG CC^T[ACGT]AGG CelII 2274 GCTTAGC GC^T[ACGT]AGC Cfr10I 1349 GCCGGT [GA]^CCGG[CT] DraIII 2134 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2259 CCTGAGG CC^T[ACGT]AGG EcoRI 451 GAATTC G^AATTC EcoT22I 2235 ATGCAT ATGCA^T FbaI 2417 TGATCA T^GATCA Hin1I 2356 GGCGTC G[GA]^CG[CT]C MroI 14 TCCGGA T^CCGGA NdeI 1684 CATATG CA^TATG NsiI 2235 ATGCAT ATGCA^T PflFI 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 742 CACGTG CAC^GTG PmlI 742 CACGTG CAC^GTG Psp1406I 424 AACGTT AA^CGTT ScaI 2222 AGTACT AGT^ACT StuI 96 AGGCCT AGG^CCT Tth111I 1516 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 282 TCTAGA T^CTAGA XcmI 2136 CCAGGTGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccI 883 GTCTAC GT^[AC][GT]AC 2528 GTCTAC AlwNI 499 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1006 CAGGACCTG AseI 214 ATTAAT AT^TAAT 2193 ATTAAT AsnI 214 ATTAAT AT^TAAT 2193 ATTAAT Asp700I 1734 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2478 GAACAAATTC BalI 702 TGGCCA TGG^CCA 1616 TGGCCA BamHI 1106 GGATCC G^GATCC 1551 GGATCC BbvCI 75 CCTCAGC CCTCAGC(-5/-2) 976 GCTGAGG BfaI 181 CTAG C^TAG 283 CTAG BsaI 22 GAGACC GGTCTC(1/5) 113 GAGACC BsaWI 14 TCCGGA [AT]^CCGG[AT] 2051 ACCGGA BseRI 769 GAGGAG GAGGAG(10/8) 1027 GAGGAG BsmI 1559 GCATTC GAATGC(1/-1) 1865 GCATTC Bsp1407I 1385 TGTACA T^GTACA 1463 TGTACA BspHI 1067 TCATGA T^CATGA 1328 TCATGA BspLU11I 938 ACATGT A^CATGT 1886 ACATGT BsrDI 972 CATTGC GCAATG(2/0) 2099 CATTGC BsrGI 1385 TGTACA T^GTACA 1463 TGTACA Cac8I 250 GCCAGC GC[ACGT]^[ACGT]GC 2497 GCAGGC EcoT14I 543 CCAAGG C^C[AT][AT]GG 1590 CCTTGG FauI 803 CCCGC CCCGC(4/6) 1483 CCCGC HaeII 79 AGCGCC [GA]GCGC^[CT] 2271 GGCGCT HgaI 1520 GCGTC GACGC(5/10) 2357 GCGTC HincII 1426 GTCAAC GT[CT]^[GA]AC 2341 GTTGAC HindII 1426 GTCAAC GT[CT]^[GA]AC 2341 GTTGAC MaeI 181 CTAG C^TAG 283 CTAG MluNI 702 TGGCCA TGG^CCA 1616 TGGCCA MscI 702 TGGCCA TGG^CCA 1616 TGGCCA MspA1I 489 CAGCTG C[AC]G^C[GT]G 1546 CAGCTG PaeR7I 310 CTCGAG C^TCGAG 1035 CTCGAG PpuMI 4 GGGTCCC [GA]G^G[AT]CC[CT] 1007 AGGACCT PshBI 214 ATTAAT AT^TAAT 2193 ATTAAT PstI 49 CTGCAG CTGCA^G 1003 CTGCAG PvuII 489 CAGCTG CAG^CTG 1546 CAGCTG RcaI 1067 TCATGA T^CATGA 1328 TCATGA SapI 175 GAAGAGC GCTCTTC(1/4) 42 GCTCTTC SexAI 1751 ACCTGGT A^CC[AT]GGT 2135 ACCAGGT SmlI 310 CTCGAG C^T[CT][GA]AG 1035 CTCGAG SspBI 1385 TGTACA T^GTACA 1463 TGTACA StyI 543 CCAAGG C^C[AT][AT]GG 1590 CCTTGG XhoI 310 CTCGAG C^TCGAG 1035 CTCGAG XmnI 1734 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2478 GAACAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AvaI AvaII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII