(Circular) (Six-base) MAPSORT of: pFN21AE6747.seq Check: 3101 from: 1 to: 7273 pFN21AE6747 7273 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5083 278 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2889 4534 4616 1937 Size: 952 1645 82 4594 Fragments arranged by size: 4594 1645 952 82 Acc65I G'GTAC_C Cuts at: 4594 4594 Size: 7273 AclI AA'CG_TT Cuts at: 1563 2430 5400 5773 1563 Size: 867 2970 373 3063 Fragments arranged by size: 3063 2970 867 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 3718 5336 6720 6988 1632 Size: 633 1453 1618 1384 268 1917 Fragments arranged by size: 1917 1618 1453 1384 633 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7076 AflIII A'CryG_T Cuts at: 1203 2748 2943 3456 3891 6172 7149 1203 Size: 1545 195 513 435 2281 977 1327 Fragments arranged by size: 2281 1545 1327 977 513 435 195 AgeI A'CCGG_T Cuts at: 6104 6104 Size: 7273 AhdI GACnn_n'nnGTC Cuts at: 6002 6002 Size: 7273 AleI CACnn'nnGTG Cuts at: 1166 2606 1166 Size: 1440 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7273 AlwNI CAG_nnn'CTG Cuts at: 2509 3016 5016 6588 2509 Size: 507 2000 1572 3194 Fragments arranged by size: 3194 2000 1572 507 ApaLI G'TGCA_C Cuts at: 5330 6486 5330 Size: 1156 6117 ApoI r'AATT_y Cuts at: 1493 2153 2456 3125 3881 4475 4487 4511 4582 Size: 660 303 669 756 594 12 24 71 Cuts at: 4582 4808 4960 7069 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 756 669 660 594 303 226 152 71 24 12 AseI AT'TA_AT Cuts at: 160 1032 2220 4199 5827 160 Size: 872 1188 1979 1628 1606 Fragments arranged by size: 1979 1628 1606 1188 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 AvaI C'yCGr_G Cuts at: 1870 1943 2315 2996 3040 4598 1870 Size: 73 372 681 44 1558 4545 Fragments arranged by size: 4545 1558 681 372 73 44 BamHI G'GATC_C Cuts at: 3111 3556 4603 4970 3111 Size: 445 1047 367 5414 Fragments arranged by size: 5414 1047 445 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4505 4594 6049 618 Size: 325 217 627 2718 89 1455 1842 Fragments arranged by size: 2718 1842 1455 627 325 217 89 BanII G_rGCy'C Cuts at: 1391 3041 4592 1391 Size: 1650 1551 4072 Fragments arranged by size: 4072 1650 1551 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2646 928 Size: 971 747 5555 Fragments arranged by size: 5555 971 747 BbvCI CC'TCA_GC Cuts at: 2081 2982 2081 Size: 901 6372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5464 5498 1903 Size: 34 3527 34 3678 Fragments arranged by size: 3678 3527 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5165 6375 3794 Size: 1371 1210 4692 Fragments arranged by size: 4692 1371 1210 BclI T'GATC_A Cuts at: 1548 4422 1548 Size: 2874 4399 BfrBI ATG'CAT Cuts at: 4242 4242 Size: 7273 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5884 136 Size: 107 122 71 631 605 4212 1525 Fragments arranged by size: 4212 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7268 7268 Size: 7273 BlpI GC'TnA_GC Cuts at: 4280 4690 4280 Size: 410 6863 Bme1580I G_kGCm'C Cuts at: 1790 2066 3028 5334 6490 1790 Size: 276 962 2306 1156 2573 Fragments arranged by size: 2573 2306 1156 962 276 BmgBI CAC'GTC Cuts at: 1318 2959 1318 Size: 1641 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2531 4477 5004 Size: 416 53 83 399 24 1114 1946 527 Cuts at: 5004 5962 442 Size: 958 1753 Fragments arranged by size: 1946 1753 1114 958 527 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7273 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3055 Size: 7273 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3050 3460 3591 3838 5933 1239 Size: 1811 410 131 247 2095 2579 Fragments arranged by size: 2579 2095 1811 410 247 131 Bpu10I CC'TnA_GC Cuts at: 2081 2982 2081 Size: 901 6372 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5398 6263 6561 5398 Size: 865 298 6110 Fragments arranged by size: 6110 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2021 2112 5936 882 Size: 617 522 91 3824 2219 Fragments arranged by size: 3824 2219 617 522 91 BsaAI yAC'GTr Cuts at: 493 1204 2749 5064 493 Size: 711 1545 2315 2702 Fragments arranged by size: 2702 2315 1545 711 BsaBI GATnn'nnATC Cuts at: 7267 7267 Size: 7273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4362 5080 5462 6097 Size: 53 83 186 936 2829 718 382 635 Cuts at: 6097 275 Size: 1451 Fragments arranged by size: 2829 1451 936 718 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 4056 5706 6104 6378 6525 6936 2019 Size: 2037 1650 398 274 147 411 2356 Fragments arranged by size: 2356 2037 1650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4241 4271 1178 Size: 30 165 30 2838 30 4180 Fragments arranged by size: 4180 2838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 3047 1186 Size: 1603 258 5412 Fragments arranged by size: 5412 1603 258 BseYI C'CCAG_C Cuts at: 2509 3866 3904 4108 6476 6972 2509 Size: 1357 38 204 2368 496 2810 Fragments arranged by size: 2810 2368 1357 496 204 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 2968 4521 1163 Size: 573 1083 149 1553 3915 Fragments arranged by size: 3915 1553 1083 573 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4723 5484 6512 1540 Size: 275 186 2722 761 1028 2301 Fragments arranged by size: 2722 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5334 5419 6490 5334 Size: 85 1071 6117 Fragments arranged by size: 6117 1071 85 BsmI GAATG_Cn' Cuts at: 3564 3870 4784 4877 3564 Size: 306 914 93 5960 Fragments arranged by size: 5960 914 306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 3028 3041 4592 5334 5419 6490 Size: 399 276 962 13 1551 742 85 1071 Cuts at: 6490 1391 Size: 2174 Fragments arranged by size: 2174 1551 1071 962 742 399 276 85 13 BspEI T'CCGG_A Cuts at: 2019 6936 2019 Size: 4917 2356 BspHI T'CATG_A Cuts at: 3072 3333 5162 6892 3072 Size: 261 1829 1730 3453 Fragments arranged by size: 3453 1829 1730 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3024 4628 844 Size: 2180 1604 3489 Fragments arranged by size: 3489 2180 1604 BsrBI CCG'CTC Cuts at: 1394 1823 2779 5160 1394 Size: 429 956 2381 3507 Fragments arranged by size: 3507 2381 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2976 4103 5768 5942 65 Size: 1183 1728 1127 1665 174 1396 Fragments arranged by size: 1728 1665 1396 1183 1127 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3354 5917 6104 1700 Size: 110 1544 2563 187 2869 Fragments arranged by size: 2869 2563 1544 187 110 BsrGI T'GTAC_A Cuts at: 96 3390 3468 96 Size: 3294 78 3901 Fragments arranged by size: 3901 3294 78 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7273 BssSI C'ACGA_G Cuts at: 5333 6345 5333 Size: 1012 6261 BstAPI GCAn_nnn'nTGC Cuts at: 794 3016 794 Size: 2222 5051 BstBI TT'CG_AA Cuts at: 6077 6077 Size: 7273 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7273 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2038 Size: 7273 BstYI r'GATC_y Cuts at: 1972 2787 2818 3004 3111 3556 4603 4970 5355 Size: 815 31 186 107 445 1047 367 385 Cuts at: 5355 5372 6824 6910 6922 7268 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1047 815 445 385 367 346 186 107 86 31 17 12 Bsu36I CC'TnA_GG Cuts at: 4265 4265 Size: 7273 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5782 Fragments arranged by size: 5782 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6534 Fragments arranged by size: 6534 683 56 BtsI GCAGTG_nn' Cuts at: 2740 4789 5583 5603 2740 Size: 2049 794 20 4410 Fragments arranged by size: 4410 2049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3996 4031 529 Size: 35 3432 35 3771 Fragments arranged by size: 3771 3432 35 35 DraI TTT'AAA Cuts at: 4577 4577 Size: 7273 DraIII CAC_nnn'GTG Cuts at: 4144 4144 Size: 7273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6280 809 Size: 507 4964 1802 Fragments arranged by size: 4964 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2707 3352 3462 Size: 54 1425 50 275 18 877 645 110 Cuts at: 3462 3621 4720 5609 7238 8 Size: 159 1099 889 1629 43 Fragments arranged by size: 1629 1425 1099 889 877 645 275 159 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4720 1537 Size: 275 2908 4090 Fragments arranged by size: 4090 2908 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2165 2175 3785 3998 4122 4170 5203 Size: 701 111 10 1610 213 124 48 1033 Cuts at: 5203 1353 Size: 3423 Fragments arranged by size: 3423 1610 1033 701 213 124 111 48 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3017 5860 6234 6380 387 Size: 1064 138 1428 2843 374 146 1280 Fragments arranged by size: 2843 1428 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 3050 3460 3591 3718 3838 5336 Size: 393 633 785 410 131 127 120 1498 Cuts at: 5336 5933 6720 6988 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1498 787 785 633 597 410 393 268 131 127 120 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2649 4264 2279 Size: 370 1615 5288 Fragments arranged by size: 5288 1615 370 EcoO109I rG'GnC_Cy Cuts at: 2010 3013 3584 2010 Size: 1003 571 5699 Fragments arranged by size: 5699 1003 571 EcoRI G'AATT_C Cuts at: 2456 4582 4960 2456 Size: 2126 378 4769 Fragments arranged by size: 4769 2126 378 FspI TGC'GCA Cuts at: 5779 5779 Size: 7273 HaeII r_GCGC'y Cuts at: 1678 2088 4280 6420 1678 Size: 410 2192 2140 2531 Fragments arranged by size: 2531 2192 2140 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5914 5946 5988 6020 1373 Size: 32 135 32 4342 32 42 32 2626 Fragments arranged by size: 4342 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3433 4348 4617 4863 669 Size: 988 281 1495 915 269 246 3079 Fragments arranged by size: 3079 1495 988 915 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7273 HpaI GTT'AAC Cuts at: 4863 4863 Size: 7273 KpnI G_GTAC'C Cuts at: 4598 4598 Size: 7273 MfeI C'AATT_G Cuts at: 4872 4872 Size: 7273 MluI A'CGCG_T Cuts at: 7149 7149 Size: 7273 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 3148 4272 6387 6571 1305 Size: 1161 682 1124 2115 184 2007 Fragments arranged by size: 2115 2007 1161 1124 682 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2709 3623 10 Size: 54 1425 343 877 914 3660 Fragments arranged by size: 3660 1425 914 877 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 2606 3338 3689 4325 5231 5590 Size: 648 1227 213 732 351 636 906 359 Cuts at: 5590 5749 518 Size: 159 2042 Fragments arranged by size: 2042 1227 906 732 648 636 359 351 213 159 MspA1I CmG'CkG Cuts at: 1606 2496 3553 4689 5366 6514 6759 1606 Size: 890 1057 1136 677 1148 245 2120 Fragments arranged by size: 2120 1148 1136 1057 890 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5782 Fragments arranged by size: 5782 939 552 NdeI CA'TA_TG Cuts at: 387 3690 387 Size: 3303 3970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7273 NotI GC'GGCC_GC Cuts at: 4720 4720 Size: 7273 NsiI A_TGCA'T Cuts at: 4244 4244 Size: 7273 NspI r_CATG'y Cuts at: 1132 1229 2398 2938 2947 3895 4631 6176 1132 Size: 97 1169 540 9 948 736 1545 2229 Fragments arranged by size: 2229 1545 1169 948 736 540 97 9 PasI CC'CwG_GG Cuts at: 1348 2089 1348 Size: 741 6532 PciI A'CATG_T Cuts at: 2943 3891 6172 2943 Size: 948 2281 4044 Fragments arranged by size: 4044 2281 948 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7273 PfoI T'CCnGG_A Cuts at: 2790 2814 3568 3768 2790 Size: 24 754 200 6295 Fragments arranged by size: 6295 754 200 24 PmeI GTTT'AAAC Cuts at: 4577 4577 Size: 7273 PmlI CAC'GTG Cuts at: 2749 2749 Size: 7273 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5342 5374 6855 6887 5342 Size: 32 1481 32 5728 Fragments arranged by size: 5728 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 3013 2010 Size: 1003 6270 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7273 PsiI TTA'TAA Cuts at: 4076 4843 4076 Size: 767 6506 PspXI vC'TCGA_Gb Cuts at: 2315 2315 Size: 7273 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4475 4507 4475 Size: 32 7241 PstI C_TGCA'G Cuts at: 830 2058 3012 4625 830 Size: 1228 954 1613 3478 Fragments arranged by size: 3478 1613 1228 954 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7273 PvuII CAG'CTG Cuts at: 2496 3553 2496 Size: 1057 6216 SalI G'TCGA_C Cuts at: 1936 4615 1936 Size: 2679 4594 SanDI GG'GwC_CC Cuts at: 2010 2010 Size: 7273 SapI GCTCTTCn'nnn_ Cuts at: 2054 2175 2054 Size: 121 7152 SbfI CC_TGCA'GG Cuts at: 4625 4625 Size: 7273 ScaI AGT'ACT Cuts at: 4229 5521 7233 4229 Size: 1292 1712 4269 Fragments arranged by size: 4269 1712 1292 SexAI A'CCwGG_T Cuts at: 3756 4140 3756 Size: 384 6889 SfcI C'TryA_G Cuts at: 826 1045 2054 2890 3008 3175 4621 5756 6437 Size: 219 1009 836 118 167 1446 1135 681 Cuts at: 6437 6628 826 Size: 191 1471 Fragments arranged by size: 1471 1446 1135 1009 836 681 219 191 167 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7273 SmaI CCC'GGG Cuts at: 1872 4600 1872 Size: 2728 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 3040 5377 6278 6540 820 Size: 197 926 372 725 2337 901 262 1553 Fragments arranged by size: 2337 1553 926 901 725 372 262 197 SnaBI TAC'GTA Cuts at: 493 5064 493 Size: 4571 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7273 SphI G_CATG'C Cuts at: 1132 4631 1132 Size: 3499 3774 SspI AAT'ATT Cuts at: 5 52 2196 3717 4197 5197 5 Size: 47 2144 1521 480 1000 2081 Fragments arranged by size: 2144 2081 1521 1000 480 47 StuI AGG'CCT Cuts at: 1517 2103 1517 Size: 586 6687 StyI C'CwwG_G Cuts at: 513 1065 2004 2548 3595 4712 513 Size: 552 939 544 1047 1117 3074 Fragments arranged by size: 3074 1117 1047 939 552 544 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3438 3498 5300 5317 5470 5655 6100 Size: 1255 1858 60 1802 17 153 185 445 Cuts at: 6100 325 Size: 1498 Fragments arranged by size: 1858 1802 1498 1255 445 185 153 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 2830 3390 Size: 274 80 33 51 1443 408 445 560 Cuts at: 3390 3468 4227 5519 7231 96 Size: 78 759 1292 1712 138 Fragments arranged by size: 1712 1443 1292 759 560 445 408 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4072 4104 7233 7265 4072 Size: 32 3129 32 4080 Fragments arranged by size: 4080 3129 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3524 1316 Size: 2208 5065 XbaI T'CTAG_A Cuts at: 2287 4609 2287 Size: 2322 4951 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4148 4148 Size: 7273 XhoI C'TCGA_G Cuts at: 1943 2315 3040 1943 Size: 372 725 6176 Fragments arranged by size: 6176 725 372 XmaI C'CCGG_G Cuts at: 1870 4598 1870 Size: 2728 4545 XmnI GAAnn'nnTTC Cuts at: 3743 4487 5402 3743 Size: 744 915 5614 Fragments arranged by size: 5614 915 744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5081 276 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI RsrII SacI SacII SfiI SfoI SrfI SwaI