(Linear) (Six-base) MAPSORT of: KIEE6747.seq Check: 8922 from: 1 to: 2568 KIEE6747.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 884 2529 2568 Size: 884 1645 39 Fragments arranged by size: 1645 884 39 AclI AA'CG_TT Cuts at: 0 425 2568 Size: 425 2143 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1713 2568 Size: 260 1453 855 Fragments arranged by size: 1453 855 260 AflIII A'CryG_T Cuts at: 0 743 938 1451 1886 2568 Size: 743 195 513 435 682 Fragments arranged by size: 743 682 513 435 195 AleI CACnn'nnGTG Cuts at: 0 601 2568 Size: 601 1967 AlwNI CAG_nnn'CTG Cuts at: 0 504 1011 2568 Size: 504 507 1557 Fragments arranged by size: 1557 507 504 ApoI r'AATT_y Cuts at: 0 148 451 1120 1876 2470 2482 2506 2568 Size: 148 303 669 756 594 12 24 62 Fragments arranged by size: 756 669 594 303 148 62 24 12 AseI AT'TA_AT Cuts at: 0 215 2194 2568 Size: 215 1979 374 Fragments arranged by size: 1979 374 215 AvaI C'yCGr_G Cuts at: 0 310 991 1035 2568 Size: 310 681 44 1533 Fragments arranged by size: 1533 681 310 44 BamHI G'GATC_C Cuts at: 0 1106 1551 2568 Size: 1106 445 1017 Fragments arranged by size: 1106 1017 445 BanI G'GyrC_C Cuts at: 0 2500 2568 Size: 2500 68 BanII G_rGCy'C Cuts at: 0 1036 2568 Size: 1036 1532 BbsI GAAGACnn'nnnn_ Cuts at: 0 641 2568 Size: 641 1927 BbvCI CC'TCA_GC Cuts at: 0 76 977 2568 Size: 76 901 1591 Fragments arranged by size: 1591 901 76 BciVI GTATCCnnnnn_n' Cuts at: 0 1789 2568 Size: 1789 779 BclI T'GATC_A Cuts at: 0 2417 2568 Size: 2417 151 BfrBI ATG'CAT Cuts at: 0 2237 2568 Size: 2237 331 BlpI GC'TnA_GC Cuts at: 0 2275 2568 Size: 2275 293 Bme1580I G_kGCm'C Cuts at: 0 61 1023 2568 Size: 61 962 1545 Fragments arranged by size: 1545 962 61 BmgBI CAC'GTC Cuts at: 0 954 2568 Size: 954 1614 BmrI ACTGGGnnnn_n' Cuts at: 0 526 2472 2568 Size: 526 1946 96 Fragments arranged by size: 1946 526 96 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1050 2568 Size: 1050 1518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1045 1455 1586 1833 2568 Size: 1045 410 131 247 735 Fragments arranged by size: 1045 735 410 247 131 Bpu10I CC'TnA_GC Cuts at: 0 76 977 2568 Size: 76 901 1591 Fragments arranged by size: 1591 901 76 BsaI GGTCTCn'nnnn_ Cuts at: 0 16 107 2568 Size: 16 91 2461 Fragments arranged by size: 2461 91 16 BsaAI yAC'GTr Cuts at: 0 744 2568 Size: 744 1824 BsaHI Gr'CG_yC Cuts at: 0 2357 2568 Size: 2357 211 BsaWI w'CCGG_w Cuts at: 0 14 2051 2568 Size: 14 2037 517 Fragments arranged by size: 2037 517 14 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2236 2266 2568 Size: 2236 30 302 Fragments arranged by size: 2236 302 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 784 1042 2568 Size: 784 258 1526 Fragments arranged by size: 1526 784 258 BseYI C'CCAG_C Cuts at: 0 504 1861 1899 2103 2568 Size: 504 1357 38 204 465 Fragments arranged by size: 1357 504 465 204 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 963 2516 2568 Size: 814 149 1553 52 Fragments arranged by size: 1553 814 149 52 BsmI GAATG_Cn' Cuts at: 0 1559 1865 2568 Size: 1559 306 703 Fragments arranged by size: 1559 703 306 Bsp1286I G_dGCh'C Cuts at: 0 61 1023 1036 2568 Size: 61 962 13 1532 Fragments arranged by size: 1532 962 61 13 BspEI T'CCGG_A Cuts at: 0 14 2568 Size: 14 2554 BspHI T'CATG_A Cuts at: 0 1067 1328 2568 Size: 1067 261 1240 Fragments arranged by size: 1240 1067 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1019 2568 Size: 1019 1549 BsrBI CCG'CTC Cuts at: 0 774 2568 Size: 774 1794 BsrDI GCAATG_nn' Cuts at: 0 971 2098 2568 Size: 971 1127 470 Fragments arranged by size: 1127 971 470 BsrFI r'CCGG_y Cuts at: 0 1349 2568 Size: 1349 1219 BsrGI T'GTAC_A Cuts at: 0 1385 1463 2568 Size: 1385 78 1105 Fragments arranged by size: 1385 1105 78 BstAPI GCAn_nnn'nTGC Cuts at: 0 1011 2568 Size: 1011 1557 BstXI CCAn_nnnn'nTGG Cuts at: 0 33 2568 Size: 33 2535 BstYI r'GATC_y Cuts at: 0 782 813 999 1106 1551 2568 Size: 782 31 186 107 445 1017 Fragments arranged by size: 1017 782 445 186 107 31 Bsu36I CC'TnA_GG Cuts at: 0 2260 2568 Size: 2260 308 BtsI GCAGTG_nn' Cuts at: 0 735 2568 Size: 735 1833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1991 2026 2568 Size: 1991 35 542 Fragments arranged by size: 1991 542 35 DraIII CAC_nnn'GTG Cuts at: 0 2139 2568 Size: 2139 429 EaeI y'GGCC_r Cuts at: 0 702 1347 1457 1616 2568 Size: 702 645 110 159 952 Fragments arranged by size: 952 702 645 159 110 EarI CTCTTCn'nnn_ Cuts at: 0 49 160 170 1780 1993 2117 2165 2568 Size: 49 111 10 1610 213 124 48 403 Fragments arranged by size: 1610 403 213 124 111 49 48 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1012 2568 Size: 1012 1556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1045 1455 1586 1713 1833 2568 Size: 260 785 410 131 127 120 735 Fragments arranged by size: 785 735 410 260 131 127 120 EcoNI CCTnn'n_nnAGG Cuts at: 0 274 644 2259 2568 Size: 274 370 1615 309 Fragments arranged by size: 1615 370 309 274 EcoO109I rG'GnC_Cy Cuts at: 0 5 1008 1579 2568 Size: 5 1003 571 989 Fragments arranged by size: 1003 989 571 5 EcoRI G'AATT_C Cuts at: 0 451 2568 Size: 451 2117 HaeII r_GCGC'y Cuts at: 0 83 2275 2568 Size: 83 2192 293 Fragments arranged by size: 2192 293 83 HincII GTy'rAC Cuts at: 0 1428 2343 2568 Size: 1428 915 225 Fragments arranged by size: 1428 915 225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 461 1143 2267 2568 Size: 461 682 1124 301 Fragments arranged by size: 1124 682 461 301 MscI TGG'CCA Cuts at: 0 704 1618 2568 Size: 704 914 950 Fragments arranged by size: 950 914 704 MslI CAynn'nnrTG Cuts at: 0 388 601 1333 1684 2320 2568 Size: 388 213 732 351 636 248 Fragments arranged by size: 732 636 388 351 248 213 MspA1I CmG'CkG Cuts at: 0 491 1548 2568 Size: 491 1057 1020 Fragments arranged by size: 1057 1020 491 NdeI CA'TA_TG Cuts at: 0 1685 2568 Size: 1685 883 NsiI A_TGCA'T Cuts at: 0 2239 2568 Size: 2239 329 NspI r_CATG'y Cuts at: 0 393 933 942 1890 2568 Size: 393 540 9 948 678 Fragments arranged by size: 948 678 540 393 9 PasI CC'CwG_GG Cuts at: 0 84 2568 Size: 84 2484 PciI A'CATG_T Cuts at: 0 938 1886 2568 Size: 938 948 682 Fragments arranged by size: 948 938 682 PfoI T'CCnGG_A Cuts at: 0 785 809 1563 1763 2568 Size: 785 24 754 200 805 Fragments arranged by size: 805 785 754 200 24 PmlI CAC'GTG Cuts at: 0 744 2568 Size: 744 1824 PpuMI rG'GwC_Cy Cuts at: 0 5 1008 2568 Size: 5 1003 1560 Fragments arranged by size: 1560 1003 5 PsiI TTA'TAA Cuts at: 0 2071 2568 Size: 2071 497 PspXI vC'TCGA_Gb Cuts at: 0 310 2568 Size: 310 2258 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2470 2502 2568 Size: 2470 32 66 Fragments arranged by size: 2470 66 32 PstI C_TGCA'G Cuts at: 0 53 1007 2568 Size: 53 954 1561 Fragments arranged by size: 1561 954 53 PvuII CAG'CTG Cuts at: 0 491 1548 2568 Size: 491 1057 1020 Fragments arranged by size: 1057 1020 491 SanDI GG'GwC_CC Cuts at: 0 5 2568 Size: 5 2563 SapI GCTCTTCn'nnn_ Cuts at: 0 49 170 2568 Size: 49 121 2398 Fragments arranged by size: 2398 121 49 ScaI AGT'ACT Cuts at: 0 2224 2568 Size: 2224 344 SexAI A'CCwGG_T Cuts at: 0 1751 2135 2568 Size: 1751 384 433 Fragments arranged by size: 1751 433 384 SfcI C'TryA_G Cuts at: 0 49 885 1003 1170 2568 Size: 49 836 118 167 1398 Fragments arranged by size: 1398 836 167 118 49 SmlI C'TyrA_G Cuts at: 0 310 1035 2568 Size: 310 725 1533 Fragments arranged by size: 1533 725 310 SspI AAT'ATT Cuts at: 0 191 1712 2192 2568 Size: 191 1521 480 376 Fragments arranged by size: 1521 480 376 191 StuI AGG'CCT Cuts at: 0 98 2568 Size: 98 2470 StyI C'CwwG_G Cuts at: 0 543 1590 2568 Size: 543 1047 978 Fragments arranged by size: 1047 978 543 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1433 1493 2568 Size: 1433 60 1075 Fragments arranged by size: 1433 1075 60 TatI w'GTAC_w Cuts at: 0 380 825 1385 1463 2222 2568 Size: 380 445 560 78 759 346 Fragments arranged by size: 759 560 445 380 346 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2067 2099 2568 Size: 2067 32 469 Fragments arranged by size: 2067 469 32 Tth111I GACn'n_nGTC Cuts at: 0 1519 2568 Size: 1519 1049 XbaI T'CTAG_A Cuts at: 0 282 2568 Size: 282 2286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2143 2568 Size: 2143 425 XhoI C'TCGA_G Cuts at: 0 310 1035 2568 Size: 310 725 1533 Fragments arranged by size: 1533 725 310 XmnI GAAnn'nnTTC Cuts at: 0 1738 2482 2568 Size: 1738 744 86 Fragments arranged by size: 1738 744 86 Enzymes that do cut: AccI AclI AcuI AflIII AleI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PciI PfoI PmlI PpuMI PsiI PspXI PsrI PstI PvuII SanDI SapI ScaI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BglI BglII BmtI BpuEI BsaBI BsiEI BsiHKAI BsiWI BsmBI BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI ClaI DraI DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PflMI PmeI PpiI PshAI PspOMI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XmaI ZraI