Restriction map for
pFN21AE4019
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 590 TCCGGA T^CCGGA
AcyI 76 GACGCC G[GA]^CG[CT]C
AspHI 25 GTGCTC G[AT]GC[AT]^C
BalI 44 TGGCCA TGG^CCA
BanI 118 GGCACC G^G[CT][GA]CC
BbvI 247 GCTGC GCAGC(8/12)
BcgI 75 CGACGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 398 TGATCA T^GATCA
BcnI 459 CCGGG CC^[GC]GG
BglII 560 AGATCT A^GATCT
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsaI 51 GGTCTC GGTCTC(1/5)
BseAI 590 TCCGGA T^CCGGA
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
BsiYI 42 CCTGGCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 42 CCTGGCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 555 GGGAC GGGAC(10/14)
BspEI 590 TCCGGA T^CCGGA
BsrI 227 CCAGT ACTGG(1/-1)
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
BstXI 545 CCATGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 560 AGATCT [GA]^GATC[CT]
DsaI 47 CCACGG C^C[GA][CT]GG
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
FauI 105 GCGGG CCCGC(4/6)
FbaI 398 TGATCA T^GATCA
Hin1I 76 GACGCC G[GA]^CG[CT]C
MaeII 173 ACGT A^CGT
MflI 560 AGATCT [GA]^GATC[CT]
MluNI 44 TGGCCA TGG^CCA
MroI 590 TCCGGA T^CCGGA
MscI 44 TGGCCA TGG^CCA
MspA1I 425 CAGCTG C[AC]G^C[GT]G
NciI 459 CCGGG CC^[GC]GG
NheI 408 GCTAGC G^CTAGC
PleI 303 GACTC GAGTC(4/5)
PvuII 425 CAGCTG CAG^CTG
SfaNI 71 GCATC GCATC(5/9)
TfiI 223 GAATC G^A[AT]TC
TseI 247 GCTGC G^C[AT]GC
XhoII 560 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 203 GAATTT [GA]^AATT[CT]
271 AAATTT
ApoI 203 GAATTT [GA]^AATT[CT]
271 AAATTT
AvaI 54 CTCGGG C^[CT]CG[GA]G
114 CTCGGG
BmyI 25 GTGCTC G[AGT]GC[ACT]^C
117 GGGCAC
BsaJI 47 CCACGG C^C[ACGT][ACGT]GG
113 CCTCGG
BsaWI 287 ACCGGA [AT]^CCGG[AT]
590 TCCGGA
BsmAI 215 GAGAC GTCTC(1/5)
52 GTCTC
BsoBI 54 CTCGGG C^[CT]CG[GA]G
114 CTCGGG
Bsp1286I 25 GTGCTC G[AGT]GC[ACT]^C
117 GGGCAC
BsrBI 35 CCGCTC CCGCTC(-3/-3)
103 GAGCGG
BstF5I 258 GGATG GGATG(2/0)
290 GGATG
Cac8I 9 GCTTGC GC[ACGT]^[ACGT]GC
408 GCTAGC
DpnII 399 GATC ^GATC
561 GATC
EaeI 32 TGGCCG [CT]^GGCC[GA]
44 TGGCCA
EarI 88 GAAGAG CTCTTC(1/4)
573 GAAGAG
FokI 258 GGATG GGATG(9/13)
290 GGATG
HgaI 6 GACGC GACGC(5/10)
76 GACGC
HinfI 223 GAATC G^A[ACGT]TC
303 GACTC
Ksp632I 88 GAAGAG CTCTTC(1/4)
573 GAAGAG
MboI 399 GATC ^GATC
561 GATC
MwoI 37 GCTCTCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
418 GCGATGACAGC
NdeII 399 GATC ^GATC
561 GATC
NlaIV 118 GGCACC GG[ACGT]^[ACGT]CC
342 GGCTCC
Sau3AI 399 GATC ^GATC
561 GATC
Tsp45I 4 GTGAC ^GT[GC]AC
109 GTCAC
Tsp509I 204 AATT ^AATT
272 AATT
TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^
413 CAGTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaII BfaI BssKI
BstNI Cfr13I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeI
MaeIII MboII MnlI MslI MspI
MvaI NlaIII Sau96I ScrFI TaqI
TthHB8I