################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MfeI MluI MroI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AspI 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 489 GGACC G^G[AT]CC BalI 179 TGGCCA TGG^CCA BamHI 419 GGATCC G^GATCC BanI 76 GGCACC G^G[CT][GA]CC BbsI 85 GAAGAC GAAGAC(2/6) BbvI 65 GCAGC GCAGC(8/12) BclI 329 TGATCA T^GATCA BcnI 633 CCGGG CC^[GC]GG BpmI 108 CTGGAG CTGGAG(16/14) BpuAI 85 GAAGAC GAAGAC(2/6) BsaI 617 GAGACC GGTCTC(1/5) BsiEI 96 CGACCG CG[GA][CT]^CG BsmAI 617 GAGAC GTCTC(1/5) BsrDI 115 GCAATG GCAATG(2/0) BsrFI 491 ACCGGC [GA]^CCGG[CT] BstBI 500 TTCGAA TT^CGAA CfoI 101 GCGC GCG^C Cfr10I 491 ACCGGC [GA]^CCGG[CT] DdeI 472 CTGAG C^T[ACGT]AG DrdI 97 GACCGCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 179 TGGCCA [CT]^GGCC[GA] EarI 639 GAAGAG CTCTTC(1/4) FbaI 329 TGATCA T^GATCA HhaI 101 GCGC GCG^C HinP1I 101 GCGC G^CGC HindIII 335 AAGCTT A^AGCTT Ksp632I 639 GAAGAG CTCTTC(1/4) MluNI 179 TGGCCA TGG^CCA MscI 179 TGGCCA TGG^CCA NciI 633 CCGGG CC^[GC]GG NspV 500 TTCGAA TT^CGAA PaeR7I 600 CTCGAG C^TCGAG PflFI 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 665 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfcI 540 CTATAG C^T[GA][CT]AG SfuI 500 TTCGAA TT^CGAA SmlI 600 CTCGAG C^T[CT][GA]AG TseI 65 GCAGC G^C[AT]GC Tsp509I 212 AATT ^AATT TspRI 592 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 268 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 665 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 600 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 100 CGCG CG^CG 102 CGCG AvaI 581 CCCGAG C^[CT]CG[GA]G 600 CTCGAG BsaBI 27 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 572 GATCTCGATC BseRI 163 CTCCTC GAGGAG(10/8) 316 GAGGAG BsoBI 581 CCCGAG C^[CT]CG[GA]G 600 CTCGAG BstF5I 243 CATCC GGATG(2/0) 26 GGATG BstNI 357 CCTGG CC^[AT]GG 665 CCAGG BstUI 100 CGCG CG^CG 102 CGCG BstYI 156 AGATCC [GA]^GATC[CT] 419 GGATCC EcoRII 357 CCTGG ^CC[AT]GG 665 CCAGG EcoT14I 38 CCAAGG C^C[AT][AT]GG 200 CCAAGG Fnu4HI 65 GCAGC GC^[ACGT]GC 249 GCGGC FokI 243 CATCC GGATG(9/13) 26 GGATG HphI 238 GGTGA GGTGA(8/7) 167 TCACC ItaI 65 GCAGC GC^[ACGT]GC 249 GCGGC MaeIII 239 GTGAC ^GT[ACGT]AC 274 GTCAC MamI 27 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 572 GATCTCGATC MflI 156 AGATCC [GA]^GATC[CT] 419 GGATCC MvaI 357 CCTGG CC^[AT]GG 665 CCAGG MvnI 100 CGCG CG^CG 102 CGCG SfaNI 27 GATGC GCATC(5/9) 346 GATGC StyI 38 CCAAGG C^C[AT][AT]GG 200 CCAAGG Tsp45I 239 GTGAC ^GT[GC]AC 274 GTCAC XhoII 156 AGATCC [GA]^GATC[CT] 419 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AlwI BsaJI BsiYI BslI BsmFI BsrI BssKI Cac8I Cfr13I DpnII FauI HaeIII HapII HinfI HpaII MboI MboII MnlI MseI MspI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI Tru9I TthHB8I