################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tsp509I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AciI 400 GCGG CCGC(-3/-1) AcyI 54 GGCGCC G[GA]^CG[CT]C AlwNI 234 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 263 CCTCAGG CC^T[ACGT]AGG AspHI 3 GAGCAC G[AT]GC[AT]^C AspI 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 54 GGCGCC G^G[CT][GA]CC BbeI 54 GGCGCC GGCGC^C BclI 303 TGATCA T^GATCA BcnI 51 CCCGG CC^[GC]GG BfaI 319 CTAG C^TAG BmyI 3 GAGCAC G[AGT]GC[ACT]^C BsaHI 54 GGCGCC G[GA]^CG[CT]C BsaWI 117 TCCGGT [AT]^CCGG[AT] BsgI 149 CTGCAC GTGCAG(16/14) BsiHKAI 3 GAGCAC G[AT]GC[AT]^C BsiYI 69 CCGGCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 69 CCGGCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 63 GGGAC GGGAC(10/14) Bsp1286I 3 GAGCAC G[AGT]GC[ACT]^C Bsu36I 263 CCTCAGG CC^T[ACGT]AGG CfoI 55 GCGC GCG^C DdeI 264 CTCAG C^T[ACGT]AG EarI 181 GAAGAG CTCTTC(1/4) Eco81I 263 CCTCAGG CC^T[ACGT]AGG FbaI 303 TGATCA T^GATCA Fnu4HI 400 GCGGC GC^[ACGT]GC HaeII 54 GGCGCC [GA]GCGC^[CT] HaeIII 214 GGCC GG^CC HhaI 55 GCGC GCG^C Hin1I 54 GGCGCC G[GA]^CG[CT]C HinP1I 55 GCGC G^CGC HphI 364 GGTGA GGTGA(8/7) ItaI 400 GCGGC GC^[ACGT]GC KasI 54 GGCGCC G^GCGCC Ksp632I 181 GAAGAG CTCTTC(1/4) MaeI 319 CTAG C^TAG MaeII 88 ACGT A^CGT MaeIII 112 GTCAC ^GT[ACGT]AC MnlI 263 CCTC CCTC(7/6) MseI 464 TTAA T^TAA NarI 54 GGCGCC GG^CGCC NciI 51 CCCGG CC^[GC]GG NcoI 58 CCATGG C^CATGG NheI 318 GCTAGC G^CTAGC NlaIII 59 CATG CATG^ PflFI 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 181 GAAGAGC GCTCTTC(1/4) SfaNI 269 GCATC GCATC(5/9) SfoI 54 GGCGCC GGC^GCC TaqI 179 TCGA T^CGA Tru9I 464 TTAA T^TAA Tsp45I 112 GTCAC ^GT[GC]AC Tth111I 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 179 TCGA T^CGA XcmI 233 CCAGCAACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BstF5I 258 CATCC GGATG(2/0) 165 GGATG Cac8I 130 GCAGGC GC[ACGT]^[ACGT]GC 318 GCTAGC DpnII 296 GATC ^GATC 304 GATC DsaI 8 CCGTGG C^C[GA][CT]GG 58 CCATGG EcoT14I 58 CCATGG C^C[AT][AT]GG 161 CCTTGG FokI 258 CATCC GGATG(9/13) 165 GGATG HinfI 65 GACTC G^A[ACGT]TC 478 GAGTC MboI 296 GATC ^GATC 304 GATC MboII 181 GAAGA GAAGA(8/7) 405 GAAGA NdeII 296 GATC ^GATC 304 GATC NlaIV 54 GGCGCC GG[ACGT]^[ACGT]CC 212 GGGGCC PleI 65 GACTC GAGTC(4/5) 478 GAGTC Sau3AI 296 GATC ^GATC 304 GATC StyI 58 CCATGG C^C[AT][AT]GG 161 CCTTGG TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^ 152 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI AvaII BsaJI BssKI BstNI Cfr13I EcoRII HapII HpaII MspI MvaI Sau96I ScrFI