(Linear) (Six-base) MAPSORT of: KIEE5133.seq  Check: 3253  from: 1  to: 498



KIEE5133.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     498 
   Size:        271     227
 
AlwNI CAG_nnn'CTG

Cuts at:      0     239     498 
   Size:        239     259
 
BanI G'GyrC_C

Cuts at:      0      54     498 
   Size:         54     444
 
BbeI G_GCGC'C

Cuts at:      0      58     498 
   Size:         58     440
 
BclI T'GATC_A

Cuts at:      0     303     498 
   Size:        303     195
 
BmtI G_CTAG'C

Cuts at:      0     322     498 
   Size:        322     176
 
BsaHI Gr'CG_yC

Cuts at:      0      55     498 
   Size:         55     443
 
BsaWI w'CCGG_w

Cuts at:      0     117     498 
   Size:        117     381
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     498 
   Size:        134     364
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     498 
   Size:          7     491
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     498 
   Size:          7     491
 
Bsu36I CC'TnA_GG

Cuts at:      0     264     498 
   Size:        264     234
 
BtgI C'CryG_G

Cuts at:      0       8      58     498 
   Size:          8      50     440

  Fragments arranged by size: 

                 440      50       8
 
BtsI GCAGTG_nn'

Cuts at:      0     151     498 
   Size:        151     347
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     498 
   Size:        176     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     498 
   Size:        271     227
 
HaeII r_GCGC'y

Cuts at:      0      58     498 
   Size:         58     440
 
KasI G'GCGC_C

Cuts at:      0      54     498 
   Size:         54     444
 
NarI GG'CG_CC

Cuts at:      0      55     498 
   Size:         55     443
 
NcoI C'CATG_G

Cuts at:      0      58     498 
   Size:         58     440
 
NheI G'CTAG_C

Cuts at:      0     318     498 
   Size:        318     180
 
SapI GCTCTTCn'nnn_

Cuts at:      0     176     498 
   Size:        176     322
 
SfoI GGC'GCC

Cuts at:      0      56     498 
   Size:         56     442
 
StyI C'CwwG_G

Cuts at:      0      58     161     498 
   Size:         58     103     337

  Fragments arranged by size: 

                 337     103      58
 
Tth111I GACn'n_nGTC

Cuts at:      0     203     498 
   Size:        203     295
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     240     498 
   Size:        240     258
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI     BbeI     BclI     BmtI    BsaHI    BsaWI
     BsgI  BsiHKAI Bsp1286I   Bsu36I     BtgI     BtsI     EarI  Eco57MI
    HaeII     KasI     NarI     NcoI     NheI     SapI     SfoI     StyI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmaI
     XmnI     ZraI