################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MluI MroI MseI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 233 GTAGAC GT^[AC][GT]AC AccII 366 CGCG CG^CG AcsI 859 AAATTC [GA]^AATT[CT] AfaI 3 GTAC GT^AC AlwNI 619 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 606 CCTGAGG CC^T[ACGT]AGG ApoI 859 AAATTC [GA]^AATT[CT] AvaII 351 GGTCC G^G[AT]CC BanI 738 GGTGCC G^G[CT][GA]CC BbsI 132 GAAGAC GAAGAC(2/6) BbvCI 789 CCTCAGC CCTCAGC(-5/-2) BcgI 95 GCAATGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 628 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 132 GAAGAC GAAGAC(2/6) Bsp1407I 2 TGTACA T^GTACA BspMI 698 ACCTGC ACCTGC(4/8) BsrGI 2 TGTACA T^GTACA BstBI 945 TTCGAA TT^CGAA BstUI 366 CGCG CG^CG Bsu36I 606 CCTGAGG CC^T[ACGT]AGG CfoI 86 GCGC GCG^C EagI 482 CGGCCG C^GGCCG EclXI 482 CGGCCG C^GGCCG Eco52I 482 CGGCCG C^GGCCG Eco81I 606 CCTGAGG CC^T[ACGT]AGG EcoT14I 768 CCATGG C^C[AT][AT]GG HhaI 86 GCGC GCG^C HinP1I 86 GCGC G^CGC MfeI 677 CAATTG C^AATTG MunI 677 CAATTG C^AATTG MvnI 366 CGCG CG^CG NcoI 768 CCATGG C^CATGG NspV 945 TTCGAA TT^CGAA PaeR7I 744 CTCGAG C^TCGAG PflMI 518 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 480 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 882 CTGCAG CTGCA^G RsaI 3 GTAC GT^AC SapI 273 GCTCTTC GCTCTTC(1/4) SfcI 882 CTGCAG C^T[GA][CT]AG SfuI 945 TTCGAA TT^CGAA SmaI 386 CCCGGG CCC^GGG SmlI 744 CTCGAG C^T[CT][GA]AG SphI 812 GCATGC GCATG^C SspBI 2 TGTACA T^GTACA StyI 768 CCATGG C^C[AT][AT]GG Van91I 518 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 744 CTCGAG C^TCGAG XmaCI 386 CCCGGG C^CCGGG XmaI 386 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### ApaI 419 GGGCCC GGGCC^C 773 GGGCCC AvaI 386 CCCGGG C^[CT]CG[GA]G 744 CTCGAG BalI 284 TGGCCA TGG^CCA 626 TGGCCA BamHI 819 GGATCC G^GATCC 1014 GGATCC BfaI 91 CTAG C^TAG 716 CTAG BpmI 454 CTGGAG CTGGAG(16/14) 547 CTGGAG BsgI 408 CTGCAC GTGCAG(16/14) 778 CTGCAC BsiEI 482 CGGCCG CG[GA][CT]^CG 954 CGGTCG BsmAI 478 GAGAC GTCTC(1/5) 487 GTCTC BsmBI 486 CGTCTC CGTCTC(1/5) 478 GAGACG BsmFI 32 GGGAC GGGAC(10/14) 251 GGGAC BsoBI 386 CCCGGG C^[CT]CG[GA]G 744 CTCGAG BsrDI 95 GCAATG GCAATG(2/0) 1008 GCAATG BsrI 239 ACTGG ACTGG(1/-1) 996 CCAGT BstAPI 401 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 959 GCAGGAGTTGC BstF5I 429 CATCC GGATG(2/0) 641 GGATG BstXI 383 CCACCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 996 CCAGTCTGATGG BstYI 819 GGATCC [GA]^GATC[CT] 1014 GGATCC DraIII 364 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG 411 CACCCTGTG DsaI 173 CCGTGG C^C[GA][CT]GG 768 CCATGG EarI 274 CTCTTC CTCTTC(1/4) 931 GAAGAG FokI 429 CATCC GGATG(9/13) 641 GGATG HinfI 511 GAGTC G^A[ACGT]TC 719 GACTC Ksp632I 274 CTCTTC CTCTTC(1/4) 931 GAAGAG MaeI 91 CTAG C^TAG 716 CTAG MaeII 231 ACGT A^CGT 280 ACGT MflI 819 GGATCC [GA]^GATC[CT] 1014 GGATCC MluNI 284 TGGCCA TGG^CCA 626 TGGCCA MscI 284 TGGCCA TGG^CCA 626 TGGCCA NspI 691 ACATGC [GA]CATG^[CT] 812 GCATGC PleI 719 GACTC GAGTC(4/5) 511 GAGTC PvuII 544 CAGCTG CAG^CTG 622 CAGCTG XhoII 819 GGATCC [GA]^GATC[CT] 1014 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I