(Circular) (Six-base) MAPSORT of: pFN21AB8802.seq Check: 1315 from: 1 to: 5743 pFN21AB8802 5743 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2239 3086 1937 Size: 302 847 4594 Fragments arranged by size: 4594 847 302 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 2393 1166 Size: 1227 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 2369 Size: 32 5711 AlwNI CAG_nnn'CTG Cuts at: 2629 3486 5058 2629 Size: 857 1572 3314 Fragments arranged by size: 3314 1572 857 ApaI G_GGCC'C Cuts at: 2428 2782 2428 Size: 354 5389 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2864 3052 3278 3430 5539 1493 Size: 1371 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1371 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2391 2749 3068 1870 Size: 73 448 358 319 4545 Fragments arranged by size: 4545 448 358 319 73 BamHI G'GATC_C Cuts at: 2824 3019 3073 3440 2824 Size: 195 54 367 5127 Fragments arranged by size: 5127 367 195 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2743 3064 4519 618 Size: 325 217 627 956 321 1455 1842 Fragments arranged by size: 1842 1455 956 627 325 321 217 BanII G_rGCy'C Cuts at: 1391 2280 2428 2782 3062 1391 Size: 889 148 354 280 4072 Fragments arranged by size: 4072 889 354 280 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 928 Size: 971 245 4527 Fragments arranged by size: 4527 971 245 BbvCI CC'TCA_GC Cuts at: 2795 2795 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2089 2123 3934 3968 1903 Size: 34 152 34 1811 34 3678 Fragments arranged by size: 3678 1811 152 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2639 4354 136 Size: 107 122 71 631 605 967 1715 1525 Fragments arranged by size: 1715 1525 967 631 605 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2428 2748 2782 3804 4960 1790 Size: 638 320 34 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 638 320 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3474 4432 Size: 416 53 83 399 24 837 1220 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1220 958 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2294 2294 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 2573 4403 1239 Size: 1241 93 1830 2579 Fragments arranged by size: 2579 1830 1241 93 Bpu10I CC'TnA_GC Cuts at: 2795 2795 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 2369 2399 1178 Size: 30 165 30 627 30 309 30 4522 Fragments arranged by size: 4522 627 309 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5743 BseYI C'CCAG_C Cuts at: 2086 2150 2708 4946 5442 2086 Size: 64 558 2238 496 2387 Fragments arranged by size: 2387 2238 558 496 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2398 2768 1163 Size: 573 662 370 4138 Fragments arranged by size: 4138 662 573 370 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2490 2962 3193 3954 4982 1540 Size: 275 186 489 472 231 761 1028 2301 Fragments arranged by size: 2301 1028 761 489 472 275 231 186 BsiHKAI G_wGCw'C Cuts at: 3804 3889 4960 3804 Size: 85 1071 4587 Fragments arranged by size: 4587 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2497 2477 Size: 20 5723 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2428 2748 2782 3062 3804 3889 Size: 399 490 148 320 34 280 742 85 Cuts at: 3889 4960 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 320 280 148 85 34 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 3098 844 Size: 1868 386 3489 Fragments arranged by size: 3489 1868 386 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2107 3020 4238 4412 65 Size: 1183 859 913 1218 174 1396 Fragments arranged by size: 1396 1218 1183 913 859 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 3832 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 2412 2970 794 Size: 1618 558 3567 Fragments arranged by size: 3567 1618 558 BstBI TT'CG_AA Cuts at: 2951 4547 2951 Size: 1596 4147 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2395 3008 2395 Size: 613 5130 BstYI r'GATC_y Cuts at: 1972 2824 3019 3073 3440 3825 3842 5294 5380 Size: 852 195 54 367 385 17 1452 86 Cuts at: 5380 5392 5738 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 852 385 367 346 195 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2612 2612 Size: 5743 BtgI C'CryG_G Cuts at: 513 1065 2178 2773 513 Size: 552 1113 595 3483 Fragments arranged by size: 3483 1113 595 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2374 2421 2374 Size: 47 5696 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2289 2487 Size: 54 1425 50 275 18 345 114 198 Cuts at: 2487 2520 2631 3190 4079 5708 8 Size: 33 111 559 889 1629 43 Fragments arranged by size: 1629 1425 889 559 345 275 198 114 111 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2487 3190 1537 Size: 275 675 703 4090 Fragments arranged by size: 4090 703 675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2931 3673 1353 Size: 932 646 742 3423 Fragments arranged by size: 3423 932 742 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2632 4330 4704 4850 387 Size: 1064 138 1043 1698 374 146 1280 Fragments arranged by size: 1698 1280 1064 1043 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2573 3806 4403 5190 5458 1239 Size: 393 848 93 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 848 787 597 393 268 93 EcoO109I rG'GnC_Cy Cuts at: 2425 2778 2779 2425 Size: 353 1 5389 Fragments arranged by size: 5389 353 1 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 4384 4416 4458 Size: 32 135 32 458 32 2322 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 2322 458 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 2682 3342 2682 Size: 660 5083 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 2633 10 Size: 54 1425 343 459 342 3120 Fragments arranged by size: 3120 1425 459 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 2672 2675 2807 2978 3701 4060 Size: 648 1227 279 3 132 171 723 359 Cuts at: 4060 4219 518 Size: 159 2042 Fragments arranged by size: 2042 1227 723 648 359 279 171 159 132 3 MspA1I CmG'CkG Cuts at: 1606 2065 2551 2629 3159 3836 4984 5229 1606 Size: 459 486 78 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 530 486 459 245 78 NcoI C'CATG_G Cuts at: 513 1065 2773 513 Size: 552 1708 3483 Fragments arranged by size: 3483 1708 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2700 2821 3101 4646 1132 Size: 97 1471 121 280 1545 2229 Fragments arranged by size: 2229 1545 1471 280 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2529 1108 Size: 1421 4322 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2369 2401 3812 3844 5325 5357 2369 Size: 32 1411 32 1481 32 2755 Fragments arranged by size: 2755 1481 1411 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2489 1613 Size: 876 4867 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2424 2778 2424 Size: 354 5389 PspXI vC'TCGA_Gb Cuts at: 2749 2749 Size: 5743 PstI C_TGCA'G Cuts at: 830 2891 3095 830 Size: 2061 204 3478 Fragments arranged by size: 3478 2061 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2551 2629 2551 Size: 78 5665 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SapI GCTCTTCn'nnn_ Cuts at: 2285 2285 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2887 3091 4226 4907 5098 826 Size: 219 1842 204 1135 681 191 1471 Fragments arranged by size: 1842 1471 1135 681 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2393 3070 1872 Size: 521 677 4545 Fragments arranged by size: 4545 677 521 SmlI C'TyrA_G Cuts at: 820 1017 1943 2749 3847 4748 5010 820 Size: 197 926 806 1098 901 262 1553 Fragments arranged by size: 1553 1098 926 901 806 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 2821 3101 1132 Size: 1689 280 3774 Fragments arranged by size: 3774 1689 280 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2773 3182 513 Size: 552 1708 409 3074 Fragments arranged by size: 3074 1708 552 409 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 3770 3787 3940 4125 4570 325 Size: 1255 1368 822 17 153 185 445 1498 Fragments arranged by size: 1498 1368 1255 822 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3989 5701 Size: 274 80 33 51 1443 30 1982 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1982 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 2749 1943 Size: 806 4937 XmaI C'CCGG_G Cuts at: 1870 2391 3068 1870 Size: 521 677 4545 Fragments arranged by size: 4545 677 521 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI