Restriction map for
pFN21AE5630
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CelII Cfr10I
ClaI DraI DraIII DrdI Eam1105I
Eco47III EcoRV EcoT22I FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 647 GGTACC G^GTACC
AccIII 1148 TCCGGA T^CCGGA
Asp718I 647 GGTACC G^GTACC
BamHI 912 GGATCC G^GATCC
BanI 647 GGTACC G^G[CT][GA]CC
BclI 530 TGATCA T^GATCA
BcnI 78 CCGGG CC^[GC]GG
BfaI 100 CTAG C^TAG
BsaI 1282 GAGACC GGTCTC(1/5)
BsaWI 1148 TCCGGA [AT]^CCGG[AT]
BseAI 1148 TCCGGA T^CCGGA
BseRI 121 GAGGAG GAGGAG(10/8)
BsiEI 917 CGGCCG CG[GA][CT]^CG
BspEI 1148 TCCGGA T^CCGGA
BssSI 1052 CACGAG CACGAG(-5/-1)
BstAPI 1223 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 630 GCGC GCG^C
CpoI 112 CGGACCG CG^G[AT]CCG
DsaI 116 CCGTGG C^C[GA][CT]GG
EagI 917 CGGCCG C^GGCCG
EclXI 917 CGGCCG C^GGCCG
Eco52I 917 CGGCCG C^GGCCG
EcoRI 1429 GAATTC G^AATTC
EcoT14I 1434 CCAAGG C^C[AT][AT]GG
FauI 177 GCGGG CCCGC(4/6)
FbaI 530 TGATCA T^GATCA
HhaI 630 GCGC GCG^C
HinP1I 630 GCGC G^CGC
KpnI 647 GGTACC GGTAC^C
MaeI 100 CTAG C^TAG
MroI 1148 TCCGGA T^CCGGA
MseI 1136 TTAA T^TAA
NciI 78 CCGGG CC^[GC]GG
PaeR7I 367 CTCGAG C^TCGAG
RsrII 112 CGGACCG CG^G[AT]CCG
SapI 618 GAAGAGC GCTCTTC(1/4)
SbfI 1446 CCTGCAGG CCTGCA^GG
ScaI 836 AGTACT AGT^ACT
SmlI 367 CTCGAG C^T[CT][GA]AG
Sse8387I 1446 CCTGCAGG CCTGCA^GG
StuI 536 AGGCCT AGG^CCT
StyI 1434 CCAAGG C^C[AT][AT]GG
TfiI 1317 GAATC G^A[AT]TC
Tru9I 1136 TTAA T^TAA
XhoI 367 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 375 CCTGAGG CC^T[ACGT]AGG
511 CCTGAGG
AspHI 231 GAGCAC G[AT]GC[AT]^C
1230 GTGCTC
AvaI 361 CTCGGG C^[CT]CG[GA]G
367 CTCGAG
BsiHKAI 231 GAGCAC G[AT]GC[AT]^C
1230 GTGCTC
BsmAI 1055 GAGAC GTCTC(1/5)
1282 GAGAC
BsoBI 361 CTCGGG C^[CT]CG[GA]G
367 CTCGAG
BstEII 450 GGTCACC G^GT[ACGT]ACC
1084 GGTTACC
BstPI 450 GGTCACC G^GT[ACGT]ACC
1084 GGTTACC
Bsu36I 375 CCTGAGG CC^T[ACGT]AGG
511 CCTGAGG
EaeI 604 CGGCCA [CT]^GGCC[GA]
917 CGGCCG
Eco81I 375 CCTGAGG CC^T[ACGT]AGG
511 CCTGAGG
EcoNI 528 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
987 CCTGAAACAGG
EcoO65I 450 GGTCACC G^GT[ACGT]ACC
1084 GGTTACC
HgaI 103 GACGC GACGC(5/10)
1179 GCGTC
MaeII 935 ACGT A^CGT
1040 ACGT
MspA1I 1032 CAGCTG C[AC]G^C[GT]G
1423 CAGCTG
NlaIII 333 CATG CATG^
762 CATG
PleI 46 GACTC GAGTC(4/5)
392 GAGTC
PvuII 1032 CAGCTG CAG^CTG
1423 CAGCTG
TaqI 368 TCGA T^CGA
1507 TCGA
Tsp45I 451 GTCAC ^GT[GC]AC
1490 GTGAC
TthHB8I 368 TCGA T^CGA
1507 TCGA
XcmI 147 CCAGGGGAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
926 CCACCCTCAACGTGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NdeII NlaIV PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp509I TspRI
XhoII