(Linear) (Six-base) MAPSORT of: KIEE5630.seq Check: 2023 from: 1 to: 1542 KIEE5630.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1217 1542 Size: 1217 325 Acc65I G'GTAC_C Cuts at: 0 647 1542 Size: 647 895 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 555 637 764 1396 1542 Size: 205 350 82 127 632 146 Fragments arranged by size: 632 350 205 146 127 82 AleI CACnn'nnGTG Cuts at: 0 457 784 1542 Size: 457 327 758 Fragments arranged by size: 758 457 327 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1012 1044 1542 Size: 1012 32 498 Fragments arranged by size: 1012 498 32 AlwNI CAG_nnn'CTG Cuts at: 0 18 66 351 987 1182 1425 1542 Size: 18 48 285 636 195 243 117 Fragments arranged by size: 636 285 243 195 117 48 18 ApoI r'AATT_y Cuts at: 0 555 588 756 1429 1542 Size: 555 33 168 673 113 Fragments arranged by size: 673 555 168 113 33 AvaI C'yCGr_G Cuts at: 0 361 367 1542 Size: 361 6 1175 Fragments arranged by size: 1175 361 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 631 664 1542 Size: 631 33 878 Fragments arranged by size: 878 631 33 BamHI G'GATC_C Cuts at: 0 912 1542 Size: 912 630 BanI G'GyrC_C Cuts at: 0 647 1542 Size: 647 895 BanII G_rGCy'C Cuts at: 0 205 368 443 477 1124 1542 Size: 205 163 75 34 647 418 Fragments arranged by size: 647 418 205 163 75 34 BbsI GAAGACnn'nnnn_ Cuts at: 0 402 755 1299 1397 1542 Size: 402 353 544 98 145 Fragments arranged by size: 544 402 353 145 98 BclI T'GATC_A Cuts at: 0 530 1542 Size: 530 1012 Bme1580I G_kGCm'C Cuts at: 0 1053 1542 Size: 1053 489 BmrI ACTGGGnnnn_n' Cuts at: 0 975 1542 Size: 975 567 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 823 1014 1300 1542 Size: 21 802 191 286 242 Fragments arranged by size: 802 286 242 191 21 Bpu10I CC'TnA_GC Cuts at: 0 1236 1542 Size: 1236 306 BsaI GGTCTCn'nnnn_ Cuts at: 0 1276 1542 Size: 1276 266 BsaWI w'CCGG_w Cuts at: 0 1148 1542 Size: 1148 394 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1012 1042 1542 Size: 1012 30 500 Fragments arranged by size: 1012 500 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 1542 Size: 136 1406 BseYI C'CCAG_C Cuts at: 0 500 1542 Size: 500 1042 BsiEI CG_ry'CG Cuts at: 0 920 1542 Size: 920 622 BsiHKAI G_wGCw'C Cuts at: 0 235 1234 1542 Size: 235 999 308 Fragments arranged by size: 999 308 235 Bsp1286I G_dGCh'C Cuts at: 0 205 235 368 443 477 1053 1124 1234 Size: 205 30 133 75 34 576 71 110 Cuts at: 1234 1542 Size: 308 Fragments arranged by size: 576 308 205 133 110 75 71 34 30 BspEI T'CCGG_A Cuts at: 0 1148 1542 Size: 1148 394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 359 803 1217 1542 Size: 359 444 414 325 Fragments arranged by size: 444 414 359 325 BssSI C'ACGA_G Cuts at: 0 1052 1542 Size: 1052 490 BstAPI GCAn_nnn'nTGC Cuts at: 0 1229 1542 Size: 1229 313 BstEII G'GTnAC_C Cuts at: 0 450 1084 1542 Size: 450 634 458 Fragments arranged by size: 634 458 450 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 789 909 1198 1542 Size: 243 546 120 289 344 Fragments arranged by size: 546 344 289 243 120 BstYI r'GATC_y Cuts at: 0 912 1250 1298 1469 1542 Size: 912 338 48 171 73 Fragments arranged by size: 912 338 171 73 48 Bsu36I CC'TnA_GG Cuts at: 0 376 512 1542 Size: 376 136 1030 Fragments arranged by size: 1030 376 136 BtgI C'CryG_G Cuts at: 0 116 1542 Size: 116 1426 BtsI GCAGTG_nn' Cuts at: 0 1209 1542 Size: 1209 333 EaeI y'GGCC_r Cuts at: 0 604 917 1542 Size: 604 313 625 Fragments arranged by size: 625 604 313 EagI C'GGCC_G Cuts at: 0 917 1542 Size: 917 625 EarI CTCTTCn'nnn_ Cuts at: 0 86 494 613 1039 1542 Size: 86 408 119 426 503 Fragments arranged by size: 503 426 408 119 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 205 555 637 764 823 1014 1300 Size: 21 184 350 82 127 59 191 286 Cuts at: 1300 1396 1542 Size: 96 146 Fragments arranged by size: 350 286 191 184 146 127 96 82 59 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 532 991 1542 Size: 532 459 551 Fragments arranged by size: 551 532 459 EcoO109I rG'GnC_Cy Cuts at: 0 348 372 481 671 974 1542 Size: 348 24 109 190 303 568 Fragments arranged by size: 568 348 303 190 109 24 EcoRI G'AATT_C Cuts at: 0 1429 1542 Size: 1429 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 944 976 1542 Size: 944 32 566 Fragments arranged by size: 944 566 32 KpnI G_GTAC'C Cuts at: 0 651 1542 Size: 651 891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 746 1542 Size: 746 796 MslI CAynn'nnrTG Cuts at: 0 305 457 784 787 1542 Size: 305 152 327 3 755 Fragments arranged by size: 755 327 305 152 3 MspA1I CmG'CkG Cuts at: 0 1034 1425 1542 Size: 1034 391 117 Fragments arranged by size: 1034 391 117 PasI CC'CwG_GG Cuts at: 0 477 1542 Size: 477 1065 PfoI T'CCnGG_A Cuts at: 0 281 549 800 908 1542 Size: 281 268 251 108 634 Fragments arranged by size: 634 281 268 251 108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1012 1044 1542 Size: 1012 32 498 Fragments arranged by size: 1012 498 32 PpuMI rG'GwC_Cy Cuts at: 0 348 372 671 1542 Size: 348 24 299 871 Fragments arranged by size: 871 348 299 24 PspXI vC'TCGA_Gb Cuts at: 0 367 1542 Size: 367 1175 PstI C_TGCA'G Cuts at: 0 71 584 1178 1451 1542 Size: 71 513 594 273 91 Fragments arranged by size: 594 513 273 91 71 PvuII CAG'CTG Cuts at: 0 1034 1425 1542 Size: 1034 391 117 Fragments arranged by size: 1034 391 117 RsrII CG'GwC_CG Cuts at: 0 113 1542 Size: 113 1429 SapI GCTCTTCn'nnn_ Cuts at: 0 613 1542 Size: 613 929 SbfI CC_TGCA'GG Cuts at: 0 1451 1542 Size: 1451 91 ScaI AGT'ACT Cuts at: 0 838 1542 Size: 838 704 SfcI C'TryA_G Cuts at: 0 67 468 580 1174 1447 1542 Size: 67 401 112 594 273 95 Fragments arranged by size: 594 401 273 112 95 67 SmlI C'TyrA_G Cuts at: 0 367 1542 Size: 367 1175 StuI AGG'CCT Cuts at: 0 538 1542 Size: 538 1004 StyI C'CwwG_G Cuts at: 0 1434 1542 Size: 1434 108 TatI w'GTAC_w Cuts at: 0 836 1542 Size: 836 706 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1043 1075 1542 Size: 1043 32 467 Fragments arranged by size: 1043 467 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 154 933 1542 Size: 154 779 609 Fragments arranged by size: 779 609 154 XhoI C'TCGA_G Cuts at: 0 367 1542 Size: 367 1175 Enzymes that do cut: AarI Acc65I AcuI AleI AloI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbsI BclI Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MslI MspA1I PasI PfoI PpiI PpuMI PspXI PstI PvuII RsrII SapI SbfI ScaI SfcI SmlI StuI StyI TatI TstI XcmI XhoI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsgI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI SacI SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XmaI XmnI ZraI