################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AluI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 133 GAATTT [GA]^AATT[CT] AfaI 147 GTAC GT^AC AflIII 35 ACATGT A^C[GA][CT]GT AlwNI 79 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 133 GAATTT [GA]^AATT[CT] BalI 242 TGGCCA TGG^CCA BclI 20 TGATCA T^GATCA BpmI 85 CTGGAG CTGGAG(16/14) BsaJI 231 CCTGGG C^C[ACGT][ACGT]GG BsiEI 6 CGACCG CG[GA][CT]^CG BspLU11I 35 ACATGT A^CATGT Cfr13I 234 GGGCC G^G[ACGT]CC DdeI 77 CTCAG C^T[ACGT]AG DpnII 21 GATC ^GATC EaeI 242 TGGCCA [CT]^GGCC[GA] EarI 43 GAAGAG CTCTTC(1/4) FbaI 20 TGATCA T^GATCA Fnu4HI 115 GCGGC GC^[ACGT]GC HaeII 81 GGCGCT [GA]GCGC^[CT] HinfI 58 GACTC G^A[ACGT]TC ItaI 115 GCGGC GC^[ACGT]GC Ksp632I 43 GAAGAG CTCTTC(1/4) MaeIII 196 GTGAC ^GT[ACGT]AC MboI 21 GATC ^GATC MluNI 242 TGGCCA TGG^CCA MnlI 177 GAGG CCTC(7/6) MscI 242 TGGCCA TGG^CCA MwoI 73 GCTACTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 21 GATC ^GATC NspI 35 ACATGT [GA]CATG^[CT] PleI 58 GACTC GAGTC(4/5) RsaI 147 GTAC GT^AC Sau3AI 21 GATC ^GATC Sau96I 234 GGGCC G^G[ACGT]CC Tsp45I 196 GTGAC ^GT[GC]AC Tsp509I 134 AATT ^AATT ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AciI 31 GCGG CCGC(-3/-1) 115 GCGG BsrDI 111 CATTGC GCAATG(2/0) 163 CATTGC BssKI 158 CCTGG ^CC[ACGT]GG 231 CCTGG BstNI 158 CCTGG CC^[AT]GG 231 CCTGG CfoI 71 GCGC GCG^C 82 GCGC EcoRII 158 CCTGG ^CC[AT]GG 231 CCTGG HhaI 71 GCGC GCG^C 82 GCGC HinP1I 71 GCGC G^CGC 82 GCGC MboII 43 GAAGA GAAGA(8/7) 249 TCTTC MvaI 158 CCTGG CC^[AT]GG 231 CCTGG NlaIII 36 CATG CATG^ 202 CATG ScrFI 158 CCTGG CC^[ACGT]GG 231 CCTGG SfaNI 48 GATGC GCATC(5/9) 167 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### HaeIII