################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccII AclI AflII AflIII AgeI AlwNI AocI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BamHI BbrPI BbsI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoRV FbaI FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MscI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 610 GACGTC GACGT^C AccI 793 GTATAC GT^[AC][GT]AC AcsI 900 GAATTC [GA]^AATT[CT] AhdI 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 736 GTGCAC G^TGCAC Aor51HI 143 AGCGCT AGC^GCT ApaI 862 GGGCCC GGGCC^C ApaLI 736 GTGCAC G^TGCAC ApoI 900 GAATTC [GA]^AATT[CT] AspEI 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 530 CCCGGG C^[CT]CG[GA]G BanI 464 GGCGCC G^G[CT][GA]CC BbeI 464 GGCGCC GGCGC^C BbvCI 580 GCTGAGG CCTCAGC(-5/-2) BciVI 209 GGATAC GTATCC(6/5) BpmI 573 CTGGAG CTGGAG(16/14) BsgI 368 CTGCAC GTGCAG(16/14) BsiEI 871 CGGTCG CG[GA][CT]^CG BsmAI 165 GAGAC GTCTC(1/5) BsoBI 530 CCCGGG C^[CT]CG[GA]G Bst1107I 793 GTATAC GTA^TAC BstEII 420 GGTGACC G^GT[ACGT]ACC BstPI 420 GGTGACC G^GT[ACGT]ACC BstZ17I 793 GTATAC GTA^TAC DrdI 21 GACTTTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 217 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 143 AGCGCT AGC^GCT EcoO65I 420 GGTGACC G^GT[ACGT]ACC EcoRI 900 GAATTC G^AATTC EcoT22I 486 ATGCAT ATGCA^T HincII 18 GTTGAC GT[CT]^[GA]AC HindII 18 GTTGAC GT[CT]^[GA]AC KasI 464 GGCGCC G^GCGCC MaeII 611 ACGT A^CGT MslI 739 CACAAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 307 CAGCGG C[AC]G^C[GT]G NarI 464 GGCGCC GG^CGCC NcoI 588 CCATGG C^CATGG NsiI 486 ATGCAT ATGCA^T PflFI 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 475 GAGCTC GAGCT^C SfcI 219 CTACAG C^T[GA][CT]AG SfoI 464 GGCGCC GGC^GCC SmaI 530 CCCGGG CCC^GGG TaqI 73 TCGA T^CGA Tth111I 493 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 73 TCGA T^CGA XmaCI 530 CCCGGG C^CCGGG XmaI 530 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccIII 287 TCCGGA T^CCGGA 635 TCCGGA AcyI 464 GGCGCC G[GA]^CG[CT]C 610 GACGTC AfaI 312 GTAC GT^AC 411 GTAC AlwI 47 GGATC GGATC(4/5) 778 GGATC BanII 475 GAGCTC G[GA]GC[CT]^C 862 GGGCCC BbvI 540 GCTGC GCAGC(8/12) 921 GCTGC BcnI 530 CCCGG CC^[GC]GG 531 CCGGG BsaHI 464 GGCGCC G[GA]^CG[CT]C 610 GACGTC BsaWI 287 TCCGGA [AT]^CCGG[AT] 635 TCCGGA BseAI 287 TCCGGA T^CCGGA 635 TCCGGA BsmFI 704 GGGAC GGGAC(10/14) 649 GTCCC BspEI 287 TCCGGA T^CCGGA 635 TCCGGA BspMI 655 ACCTGC ACCTGC(4/8) 789 GCAGGT CfoI 144 GCGC GCG^C 465 GCGC DpnII 48 GATC ^GATC 779 GATC DraII 497 GGGTCCT [GA]G^G[ACGT]CC[CT] 647 AGGTCCC DsaI 480 CCACGG C^C[GA][CT]GG 588 CCATGG EcoO109I 497 GGGTCCT [GA]G^G[ACGT]CC[CT] 647 AGGTCCC EcoT14I 449 CCAAGG C^C[AT][AT]GG 588 CCATGG FauI 248 CCCGC CCCGC(4/6) 454 GCGGG Fnu4HI 540 GCTGC GC^[ACGT]GC 921 GCTGC HaeII 143 AGCGCT [GA]GCGC^[CT] 464 GGCGCC HhaI 144 GCGC GCG^C 465 GCGC Hin1I 464 GGCGCC G[GA]^CG[CT]C 610 GACGTC HinP1I 144 GCGC G^CGC 465 GCGC ItaI 540 GCTGC GC^[ACGT]GC 921 GCTGC MboI 48 GATC ^GATC 779 GATC MroI 287 TCCGGA T^CCGGA 635 TCCGGA NciI 530 CCCGG CC^[GC]GG 531 CCGGG NdeII 48 GATC ^GATC 779 GATC PflMI 426 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCATGGCCTGG PpuMI 497 GGGTCCT [GA]G^G[AT]CC[CT] 647 AGGTCCC RsaI 312 GTAC GT^AC 411 GTAC Sau3AI 48 GATC ^GATC 779 GATC SfaNI 436 GATGC GCATC(5/9) 485 GATGC StyI 449 CCAAGG C^C[AT][AT]GG 588 CCATGG TfiI 191 GAATC G^A[AT]TC 202 GAATC TseI 540 GCTGC G^C[AT]GC 921 GCTGC Tsp45I 421 GTGAC ^GT[GC]AC 608 GTGAC Tsp509I 344 AATT ^AATT 901 AATT Van91I 426 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCATGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AspHI AvaII BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FokI HaeIII HapII HinfI HpaII HphI MaeIII MboII MnlI MspI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI TspRI