(Circular) (Six-base) MAPSORT of: pFN21AE9613.seq Check: 4865 from: 1 to: 5647 pFN21AE9613 5647 bp With 182 enzymes: SgfI * March 28, 2012 12:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3457 278 Size: 53 83 186 936 1083 838 2468 Fragments arranged by size: 2468 1083 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 2799 2990 1937 Size: 862 191 4594 Fragments arranged by size: 4594 862 191 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3710 5094 5362 1632 Size: 2078 1384 268 1917 Fragments arranged by size: 2078 1917 1384 268 AfeI AGC'GCT Cuts at: 2150 2150 Size: 5647 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 2227 4376 2227 Size: 2149 3498 AleI CACnn'nnGTG Cuts at: 1166 2748 1166 Size: 1582 4065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaI G_GGCC'C Cuts at: 2871 2871 Size: 5647 ApaLI G'TGCA_C Cuts at: 2741 3704 4860 2741 Size: 963 1156 3528 Fragments arranged by size: 3528 1156 963 ApoI r'AATT_y Cuts at: 1493 2905 2956 3182 3334 5443 1493 Size: 1412 51 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2535 2972 1870 Size: 73 592 437 4545 Fragments arranged by size: 4545 592 437 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2968 4423 618 Size: 325 217 627 682 499 1455 1842 Fragments arranged by size: 1842 1455 682 627 499 325 217 BanII G_rGCy'C Cuts at: 1391 2484 2871 2966 1391 Size: 1093 387 95 4072 Fragments arranged by size: 4072 1093 387 95 BbeI G_GCGC'C Cuts at: 2473 2473 Size: 5647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BbvCI CC'TCA_GC Cuts at: 2586 2586 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2208 3539 4749 2208 Size: 1331 1210 3106 Fragments arranged by size: 3106 1331 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BfrBI ATG'CAT Cuts at: 2493 2493 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2302 2745 2871 3708 4864 1790 Size: 512 443 126 837 1156 2573 Fragments arranged by size: 2573 1156 837 512 443 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 2329 2389 Size: 416 53 83 399 24 779 133 60 Cuts at: 2389 3378 4336 442 Size: 989 958 1753 Fragments arranged by size: 1753 989 958 779 416 399 133 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2599 4307 1239 Size: 1360 1708 2579 Fragments arranged by size: 2579 1708 1360 Bpu10I CC'TnA_GC Cuts at: 2586 2586 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2470 2616 3454 3836 Size: 53 83 186 936 937 146 838 382 Cuts at: 3836 4471 275 Size: 635 1451 Fragments arranged by size: 1451 937 936 838 635 382 186 146 83 53 BsaWI w'CCGG_w Cuts at: 2292 2640 4080 4478 4752 4899 5310 2292 Size: 348 1440 398 274 147 411 2629 Fragments arranged by size: 2629 1440 411 398 348 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2106 2344 2645 1186 Size: 920 238 301 4188 Fragments arranged by size: 4188 920 301 238 BseYI C'CCAG_C Cuts at: 2063 4850 5346 2063 Size: 2787 496 2364 Fragments arranged by size: 2787 2364 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 1163 Size: 573 622 4452 Fragments arranged by size: 4452 622 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2879 3097 3858 4886 1540 Size: 275 186 878 218 761 1028 2301 Fragments arranged by size: 2301 1028 878 761 275 218 186 BsiHKAI G_wGCw'C Cuts at: 2078 2393 2484 2745 3708 3793 4864 2078 Size: 315 91 261 963 85 1071 2861 Fragments arranged by size: 2861 1071 963 315 261 91 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2302 2393 2484 2745 2871 2966 Size: 399 288 224 91 91 261 126 95 Cuts at: 2966 3708 3793 4864 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 288 261 224 126 95 91 91 85 BspEI T'CCGG_A Cuts at: 2292 2640 5310 2292 Size: 348 2670 2629 Fragments arranged by size: 2670 2629 348 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 2785 3002 844 Size: 1825 116 217 3489 Fragments arranged by size: 3489 1825 217 116 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2425 1184 Size: 1241 4406 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2800 2800 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 2485 2593 513 Size: 552 1420 108 3567 Fragments arranged by size: 3567 1420 552 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2823 3163 3957 3977 2823 Size: 340 794 20 4493 Fragments arranged by size: 4493 794 340 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2032 4654 809 Size: 507 716 2622 1802 Fragments arranged by size: 2622 1802 716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612 Size: 54 1425 50 275 18 1264 889 1629 Cuts at: 5612 8 Size: 43 Fragments arranged by size: 1629 1425 1264 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3577 1353 Size: 2224 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2297 4234 4608 4754 387 Size: 1064 138 708 1937 374 146 1280 Fragments arranged by size: 1937 1280 1064 708 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 2482 Size: 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2599 3710 4307 5094 5362 1239 Size: 393 967 1111 597 787 268 1524 Fragments arranged by size: 1524 1111 967 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2503 2653 2503 Size: 150 5497 EcoRI G'AATT_C Cuts at: 2905 2956 3334 2905 Size: 51 378 5218 Fragments arranged by size: 5218 378 51 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2261 2032 Size: 229 5418 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2152 2473 4794 1678 Size: 474 321 2321 2531 Fragments arranged by size: 2531 2321 474 321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 4288 4320 4362 Size: 32 135 32 641 32 2043 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 641 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 2991 3237 669 Size: 988 281 87 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KasI G'GCGC_C Cuts at: 2469 2469 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2030 2859 4761 4945 1305 Size: 725 829 1902 184 2007 Fragments arranged by size: 2007 1902 829 725 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2748 3605 3964 4123 518 Size: 648 843 739 857 359 159 2042 Fragments arranged by size: 2042 857 843 739 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2314 3063 3740 4888 5133 1606 Size: 708 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 708 677 245 NarI GG'CG_CC Cuts at: 2470 2470 Size: 5647 NcoI C'CATG_G Cuts at: 513 1065 2593 513 Size: 552 1528 3567 Fragments arranged by size: 3567 1528 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NsiI A_TGCA'T Cuts at: 2495 2495 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 2599 1108 Size: 1329 162 4156 Fragments arranged by size: 4156 1329 162 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2653 2503 Size: 150 5497 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2867 2867 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SacI G_AGCT'C Cuts at: 2484 2484 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2224 2995 4130 4811 5002 826 Size: 219 1179 771 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 771 681 219 191 SfoI GGC'GCC Cuts at: 2471 2471 Size: 5647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2537 2974 1872 Size: 665 437 4545 Fragments arranged by size: 4545 665 437 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2454 2593 3086 513 Size: 552 1389 139 493 3074 Fragments arranged by size: 3074 1389 552 493 139 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3674 3691 3844 4029 4474 325 Size: 1255 2094 17 153 185 445 1498 Fragments arranged by size: 2094 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 3893 5605 Size: 274 80 33 51 1443 438 1478 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1478 1443 438 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5607 5639 5607 Size: 32 5615 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 4462 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2535 2972 1870 Size: 665 437 4545 Fragments arranged by size: 4545 665 437 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3455 276 Size: 53 83 186 936 1083 838 2468 Fragments arranged by size: 2468 1083 936 838 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI