Restriction map for
pFN21AE2465
##################################
# NO CUT ENZYMES ( 24 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AvrII BlnI BssHII CpoI DrdI
HpaI KpnI NheI NotI PacI
PinAI PmeI Psp1406I PvuI RsrII
SalI SexAI SgrAI SpeI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 12332 TCCGGA T^CCGGA
AflII 10971 CTTAAG C^TTAAG
AseI 985 ATTAAT AT^TAAT
AsnI 985 ATTAAT AT^TAAT
BbrPI 4255 CACGTG CAC^GTG
BcgI 4057 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 8067 GTATCC GTATCC(6/5)
BfrI 10971 CTTAAG C^TTAAG
BseAI 12332 TCCGGA T^CCGGA
BsiWI 4491 CGTACG C^GTACG
BspEI 12332 TCCGGA T^CCGGA
BstBI 7859 TTCGAA TT^CGAA
FseI 12387 GGCCGGCC GGCCGG^CC
HindIII 12769 AAGCTT A^AGCTT
MluI 8795 ACGCGT A^CGCGT
MroI 12332 TCCGGA T^CCGGA
NdeI 11759 CATATG CA^TATG
NruI 6697 TCGCGA TCG^CGA
NspV 7859 TTCGAA TT^CGAA
PmaCI 4255 CACGTG CAC^GTG
PmlI 4255 CACGTG CAC^GTG
PshBI 985 ATTAAT AT^TAAT
ScaI 12779 AGTACT AGT^ACT
SfuI 7859 TTCGAA TT^CGAA
SnaBI 9351 TACGTA TAC^GTA
SspI 1279 AATATT AAT^ATT
SwaI 1749 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 7183 GACGTC GACGT^C
12004 GACGTC
AhdI 869 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACTTGGAGTC
Aor51HI 6772 AGCGCT AGC^GCT
7333 AGCGCT
ApaI 9768 GGGCCC GGGCC^C
12557 GGGCCC
AspEI 869 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACTTGGAGTC
BsaAI 4255 CACGTG [CT]AC^GT[GA]
9351 TACGTA
BspDI 1423 ATCGAT AT^CGAT
11203 ATCGAT
BspHI 3488 TCATGA T^CATGA
12761 TCATGA
BssSI 4074 CACGAG CACGAG(-5/-1)
3430 CTCGTG
Bst1107I 528 GTATAC GTA^TAC
11490 GTATAC
BstZ17I 528 GTATAC GTA^TAC
11490 GTATAC
ClaI 1423 ATCGAT AT^CGAT
11203 ATCGAT
Eam1105I 869 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACTTGGAGTC
Eco47III 6772 AGCGCT AGC^GCT
7333 AGCGCT
EcoT22I 2515 ATGCAT ATGCA^T
11199 ATGCAT
HincII 8605 GTCAAC GT[CT]^[GA]AC
10482 GTCAAC
HindII 8605 GTCAAC GT[CT]^[GA]AC
10482 GTCAAC
KspI 8788 CCGCGG CCGC^GG
11345 CCGCGG
NsiI 2515 ATGCAT ATGCA^T
11199 ATGCAT
PaeR7I 10144 CTCGAG C^TCGAG
12481 CTCGAG
PshAI 4530 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
9036 GACATCCGTC
RcaI 3488 TCATGA T^CATGA
12761 TCATGA
SacII 8788 CCGCGG CCGC^GG
11345 CCGCGG
SbfI 9048 CCTGCAGG CCTGCA^GG
12081 CCTGCAGG
SfiI 4821 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
12378 GGCCGTGCGGGCC
SmaI 6791 CCCGGG CCC^GGG
8626 CCCGGG
SphI 6454 GCATGC GCATG^C
11197 GCATGC
Sse8387I 9048 CCTGCAGG CCTGCA^GG
12081 CCTGCAGG
XbaI 5941 TCTAGA T^CTAGA
6256 TCTAGA
XhoI 10144 CTCGAG C^TCGAG
12481 CTCGAG
XmaCI 6791 CCCGGG C^CCGGG
8626 CCCGGG
XmaI 6791 CCCGGG C^CCGGG
8626 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 175 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI Asp700I
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI